Expression of the functional D-glucose transport system in Xenopus oocytes injected with mRNA of rat small intestine.

Abstract:

:mRNA prepared from rat small intestine was injected into Xenopus oocytes. The injected oocytes showed a clear electrical response to D-glucose (Glu) in the form of membrane depolarization and conductance increase, while none was shown to D-fructose. The membrane electrical response of the injected oocytes evoked by Glu followed the Michaelis-Menten type kinetics and was dependent on the membrane potential of the oocyte. Replacing the Na+ of the bathing buffer with choline+ resulted in no response to Glu. Thus, a Glu transport system coupled to a Na+ gradient was expressed in Xenopus oocytes by injecting mRNA from rat small intestine.

journal_name

Brain Res

journal_title

Brain research

authors

Aoshima H,Ishii H,Anan M

doi

10.1016/0169-328x(87)90033-7

subject

Has Abstract

pub_date

1987-09-01 00:00:00

pages

263-7

issue

3

eissn

0006-8993

issn

1872-6240

journal_volume

388

pub_type

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