当前位置： SCI文献检索 > Cell Chemical Biology期刊下所有文献 > GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging of Ca(2+) Dynamics in High-Ca(2+) Organelles.

GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging of Ca(2+) Dynamics in High-Ca(2+) Organelles.

Abstract：

:Proper functioning of organelles such as the ER or the Golgi apparatus requires luminal accumulation of Ca(2+) at high concentrations. Here we describe a ratiometric low-affinity Ca(2+) sensor of the GFP-aequorin protein (GAP) family optimized for measurements in high-Ca(2+) concentration environments. Transgenic animals expressing the ER-targeted sensor allowed monitoring of Ca(2+) signals inside the organelle. The use of the sensor was demonstrated under three experimental paradigms: (1) ER Ca(2+) oscillations in cultured astrocytes, (2) ex vivo functional mapping of cholinergic receptors triggering ER Ca(2+) release in acute hippocampal slices from transgenic mice, and (3) in vivo sarcoplasmic reticulum Ca(2+) dynamics in the muscle of transgenic flies. Our results provide proof of the suitability of the new biosensors to monitor Ca(2+) dynamics inside intracellular organelles under physiological conditions and open an avenue to explore complex Ca(2+) signaling in animal models of health and disease.

journal_name

Cell Chem Biol

journal_title

Cell chemical biology

authors

Navas-Navarro P,Rojo-Ruiz J,Rodriguez-Prados M,Ganfornina MD,Looger LL,Alonso MT,García-Sancho J

doi

10.1016/j.chembiol.2016.05.010

subject

Has Abstract

pub_date

2016-06-23 00:00:00

pages

738-45

issue

6

eissn

2451-9456

issn

2451-9448

pii

S2451-9456(16)30163-5

journal_volume

23

pub_type

杂志文章

相关文献

Cell Chemical Biology文献大全
  • Functional mimicry revealed by the crystal structure of an eIF4A:RNA complex bound to the interfacial inhibitor, desmethyl pateamine A.

    abstract：:Interfacial inhibitors exert their biological effects through co-association with two macromolecules. The pateamine A (PatA) class of molecules function by stabilizing eukaryotic initiation factor (eIF) 4A RNA helicase onto RNA, resulting in translation initiation inhibition. Here, we present the crystal structure of ...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2020.12.006

    authors： Naineni SK,Liang J,Hull K,Cencic R,Zhu M,Northcote P,Teesdale-Spittle P,Romo D,Nagar B,Pelletier J

    更新日期：2021-01-05 00:00:00

  • Lipase Processing of Complex Lipid Antigens.

    abstract：:Mycobacterium tuberculosis synthesizes a wide variety of complex lipids that can serve as antigens in immune recognition of the bacterium. In this issue of Cell Chemical Biology, Gilleron et al. (2016) identify key enzymes essential for lipid antigen processing, which is required for CD1b-restricted T cell activation....

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2016.09.005

    authors： Sander P,Becker K,Molin MD

    更新日期：2016-09-22 00:00:00

  • No Bones About It: Small Molecules for Bone Regeneration.

    abstract：:In this issue of Cell Chemical Biology, Cook et al. (2019) report a new small-molecule activator that enhances osteogenesis and skeletal regeneration in developmental and adult animal models, respectively. This discovery has therapeutic potential for healing following traumatic bone injury, as well as bone remodeling ...

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2019.06.007

    authors： Chang JW,Moellering RE

    更新日期：2019-07-18 00:00:00

  • Inhibition of K-RAS4B by a Unique Mechanism of Action: Stabilizing Membrane-Dependent Occlusion of the Effector-Binding Site.

    abstract：:KRAS is frequently mutated in several of the most lethal types of cancer; however, the KRAS protein has proven a challenging drug target. K-RAS4B must be localized to the plasma membrane by prenylation to activate oncogenic signaling, thus we endeavored to target the protein-membrane interface with small-molecule comp...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.07.009

    authors： Fang Z,Marshall CB,Nishikawa T,Gossert AD,Jansen JM,Jahnke W,Ikura M

    更新日期：2018-11-15 00:00:00

  • More than One Way to Skin a Catalyst.

    abstract：:In this issue of Cell Chemical Biology, Diaz et al. (2017) report a strategy to achieve temporal, spatial, and stoichiometric control over the protein kinase cAbl in living cells. They achieve this by splitting cAbl into two inactive fragments that form an active kinase upon small molecule addition, potentially provid...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2017.10.004

    authors： Michnick SW

    更新日期：2017-10-19 00:00:00

  • Catching Sirtuin-2 Intermediates One Structure at the Time.

    abstract：:Sirtuins are a large enzyme family involved in installing and removing post-translational modifications involving lysine side chains. These enzymes have been of intense research interest and we now understand many details of their mechanism, although later steps of the deacetylase activity have remained a mystery. In ...

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2017.03.004

    authors： Lee S,Chen Z,Zhang G

    更新日期：2017-03-16 00:00:00

  • Principles of Chemical Biology: Iron and CSCs, Histone Acylation sans Enzymes, Eliciting Silent Gene Clusters, and 2'-deoxy-ADPR as a Second Messenger.

    abstract：:This month: Lysosomal iron linked to cell death in cancer stem cells, non-enzymatic catalyst SynCAc for histone acylation, cytotoxins ivermectin and etoposide bring new anti-fungals out of the crypt, and 2'-deoxy-ADPR as second messenger activating TRPM2. ...

    journal_title：Cell chemical biology

    pub_type：

    doi：10.1016/j.chembiol.2017.07.016

    authors：

    更新日期：2017-08-17 00:00:00

  • Small-Molecule-Targeting Hairpin Loop of hTERT Promoter G-Quadruplex Induces Cancer Cell Death.

    abstract：:Increased telomerase activity is associated with malignancy and poor prognosis in human cancer, but the development of targeted agents has not yet provided clinical benefit. Here we report that, instead of targeting the telomerase enzyme directly, small molecules that bind to the G-hairpin of the hTERT G-quadruplex-fo...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2019.04.009

    authors： Song JH,Kang HJ,Luevano LA,Gokhale V,Wu K,Pandey R,Sherry Chow HH,Hurley LH,Kraft AS

    更新日期：2019-08-15 00:00:00

  • Small-Molecule Kinase Downregulators.

    abstract：:New opportunities to advance small-molecule kinase ligands that downregulate their cognate target binding proteins are discussed. Rationally designed heterobifunctional kinase degraders are compared with ATP site ligands that were serendipitously found to cause kinase downregulation. These approaches could be particul...

    journal_title：Cell chemical biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.chembiol.2017.10.011

    authors： Jones LH

    更新日期：2018-01-18 00:00:00

  • Conformation Selective Antibody Enables Genome Profiling and Leads to Discovery of Parallel G-Quadruplex in Human Telomeres.

    abstract：:G-quadruplexes are specialized secondary structures in nucleic acids that possess significant conformational polymorphisms. The precise G-quadruplex conformations in vivo and their relevance to biological functions remain controversial and unclear, especially for telomeric G-quadruplexes. Here, we report a novel singl...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2016.08.013

    authors： Liu HY,Zhao Q,Zhang TP,Wu Y,Xiong YX,Wang SK,Ge YL,He JH,Lv P,Ou TM,Tan JH,Li D,Gu LQ,Ren J,Zhao Y,Huang ZS

    更新日期：2016-10-20 00:00:00

  • Concise Chemoenzymatic Total Synthesis and Identification of Cellular Targets of Cepafungin I.

    abstract：:The natural product cepafungin I was recently reported to be one of the most potent covalent inhibitors of the 20S proteasome core particle through a series of in vitro activity assays. Here, we report a short chemoenzymatic total synthesis of cepafungin I featuring the use of a regioselective enzymatic oxidation to p...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2020.07.012

    authors： Amatuni A,Shuster A,Adibekian A,Renata H

    更新日期：2020-10-15 00:00:00

  • Prevention of Serpin Misfolding by RNA Aptamers.

    abstract：:Owing to their structural flexibility, most serpins inhibit the cognate proteases in a fast and specific manner and also are susceptible to pathogenic misfolding. In this issue of Cell Chemical Biology, Madsen et al. (2016) report on the selection and characterization of an RNA aptamer that stabilizes α1-antichymotryp...

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2016.06.002

    authors： Zhou X,Declerck PJ

    更新日期：2016-06-23 00:00:00

  • A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR.

    abstract：:The rapid emergence of extensively drug-resistant A. baumannii has posed a major threat to global public health, emphasizing the desperate need for novel therapeutic strategies. We report the development of a highly efficient genome-engineering platform in A. baumannii by coupling a Cas9 nuclease-mediated genome cleav...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2019.09.003

    authors： Wang Y,Wang Z,Chen Y,Hua X,Yu Y,Ji Q

    更新日期：2019-12-19 00:00:00

  • Combined Proteomic and In Silico Target Identification Reveal a Role for 5-Lipoxygenase in Developmental Signaling Pathways.

    abstract：:Identification and validation of the targets of bioactive small molecules identified in cell-based screening is challenging and often meets with failure, calling for the development of new methodology. We demonstrate that a combination of chemical proteomics with in silico target prediction employing the SPiDER method...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.05.016

    authors： Brand S,Roy S,Schröder P,Rathmer B,Roos J,Kapoor S,Patil S,Pommerenke C,Maier T,Janning P,Eberth S,Steinhilber D,Schade D,Schneider G,Kumar K,Ziegler S,Waldmann H

    更新日期：2018-09-20 00:00:00

  • Hypomorph Mutation-Directed Small-Molecule Protein-Protein Interaction Inducers to Restore Mutant SMAD4-Suppressed TGF-β Signaling.

    abstract：:Tumor suppressor genes represent a major class of oncogenic drivers. However, direct targeting of loss-of-function tumor suppressors remains challenging. To address this gap, we explored a variant-directed chemical biology approach to reverse the lost function of tumor suppressors using SMAD4 as an example. SMAD4, a c...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2020.11.010

    authors： Tang C,Mo X,Niu Q,Wahafu A,Yang X,Qui M,Ivanov AA,Du Y,Fu H

    更新日期：2020-12-04 00:00:00

  • Targeted Degradation of a Hypoxia-Associated Non-coding RNA Enhances the Selectivity of a Small Molecule Interacting with RNA.

    abstract：:Small-molecule targeted recruitment of nucleases to RNA is a powerful method to affect RNA biology. Inforna, a sequence-based design approach to target RNA, enables the design of small molecules that bind to and cleave RNA in a selective and substoichiometric manner. Here, we investigate the ability of RNA-targeted de...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2019.04.008

    authors： Costales MG,Suresh B,Vishnu K,Disney MD

    更新日期：2019-08-15 00:00:00

  • The Convergence of Stem Cell Technologies and Phenotypic Drug Discovery.

    abstract：:Recent advances in induced pluripotent stem cell technologies and phenotypic screening shape the future of bioactive small-molecule discovery. In this review we analyze the impact of small-molecule phenotypic screens on drug discovery as well as on the investigation of human development and disease biology. We further...

    journal_title：Cell chemical biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.chembiol.2019.05.007

    authors： Friese A,Ursu A,Hochheimer A,Schöler HR,Waldmann H,Bruder JM

    更新日期：2019-08-15 00:00:00

  • A Glycoengineered Enzyme with Multiple Mannose-6-Phosphates Is Internalized into Diseased Cells to Restore Its Activity in Lysosomes.

    abstract：:In this study we developed an efficient method to prepare glycoengineered β-N-acetylhexosaminidase containing multiple mannose-6-phosphates (M6Ps) by combining genetic code expansion with bioorthogonal ligation techniques. We found that multiple M6P-conjugated enzymes were produced with a high efficiency by using comb...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.07.011

    authors： Hyun JY,Kim S,Lee HS,Shin I

    更新日期：2018-10-18 00:00:00

  • Small-Molecule TLR8 Antagonists via Structure-Based Rational Design.

    abstract：:Rational design of drug-like small-molecule ligands based on structural information of proteins remains a significant challenge in chemical biology. In particular, designs targeting protein-protein interfaces have met little success given the dynamic nature of the protein surfaces. Herein, we utilized the structure of...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.07.004

    authors： Hu Z,Tanji H,Jiang S,Zhang S,Koo K,Chan J,Sakaniwa K,Ohto U,Candia A,Shimizu T,Yin H

    更新日期：2018-10-18 00:00:00

  • "Expand and Click": A New Method for Labeling HIV-1 Envelope Glycoproteins.

    abstract：:In this issue of Cell Chemical Biology, Sakin et al. (2017) investigate the nanoscale behavior of the HIV-1 envelope (Env) glycoprotein complex by using genetic code expansion, bioorthogonal amino acids, synthetic dyes, and click chemistry. This minimally invasive approach allows the measurement of native Env cellular...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2017.05.006

    authors： Fernandez MV,Freed EO

    更新日期：2017-05-18 00:00:00

  • Epiblastin A Induces Reprogramming of Epiblast Stem Cells Into Embryonic Stem Cells by Inhibition of Casein Kinase 1.

    abstract：:The discovery of novel small molecules that induce stem cell reprogramming and give efficient access to pluripotent stem cells is of major importance for potential therapeutic applications and may reveal novel insights into the factors controlling pluripotency. Chemical reprogramming of mouse epiblast stem cells (EpiS...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2016.02.015

    authors： Ursu A,Illich DJ,Takemoto Y,Porfetye AT,Zhang M,Brockmeyer A,Janning P,Watanabe N,Osada H,Vetter IR,Ziegler S,Schöler HR,Waldmann H

    更新日期：2016-04-21 00:00:00

  • A Split-Luciferase-Based Trimer Formation Assay as a High-throughput Screening Platform for Therapeutics in Alport Syndrome.

    abstract：:Alport syndrome is a hereditary glomerular disease caused by mutation in type IV collagen α3-α5 chains (α3-α5(IV)), which disrupts trimerization, leading to glomerular basement membrane degeneration. Correcting the trimerization of α3/α4/α5 chain is a feasible therapeutic approach, but is hindered by lack of informati...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.02.003

    authors： Omachi K,Kamura M,Teramoto K,Kojima H,Yokota T,Kaseda S,Kuwazuru J,Fukuda R,Koyama K,Matsuyama S,Motomura K,Shuto T,Suico MA,Kai H

    更新日期：2018-05-17 00:00:00

  • The High Genetic Barrier of EFdA/MK-8591 Stems from Strong Interactions with the Active Site of Drug-Resistant HIV-1 Reverse Transcriptase.

    abstract：:4'-Ethynyl-2-fluoro-2'-deoxyadenosine (EFdA/MK-8591), a nucleoside reverse transcriptase inhibitor (NRTI) under clinical trials, is a potent and promising long-acting anti-HIV type 1 (HIV-1) agent. EFdA and its derivatives possess a modified 4'-moiety and potently inhibit the replication of a wide spectrum of HIV-1 st...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.07.014

    authors： Takamatsu Y,Das D,Kohgo S,Hayashi H,Delino NS,Sarafianos SG,Mitsuya H,Maeda K

    更新日期：2018-10-18 00:00:00

  • Decoding Transcriptome Dynamics of Genome-Encoded Polyadenylation and Autoregulation with Small-Molecule Modulators of Alternative Polyadenylation.

    abstract：:Alternative polyadenylation (APA) plays a critical role in regulating gene expression. However, the balance between genome-encoded APA processing and autoregulation by APA modulating RNA binding protein (RBP) factors is not well understood. We discovered two potent small-molecule modulators of APA (T4 and T5) that pro...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.09.006

    authors： Araki S,Nakayama Y,Sano O,Nakao S,Shimizu-Ogasawara M,Toyoshiba H,Nakanishi A,Aparicio S

    更新日期：2018-12-20 00:00:00

  • Dawn of a New Era of Targeted Antioxidant Therapies.

    abstract：:In this issue of Cell Chemical Biology, Shah et al. (2019) report an in vitro, high-throughput assay that predicts the ability of compounds to suppress peroxidation of phospholipids. This approach provides a way to design and optimize targeted antioxidants that suppress specific oxidative event in cells, potentially o...

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2019.11.003

    authors： Stockwell BR

    更新日期：2019-11-21 00:00:00

  • Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.

    abstract：:A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, t...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2019.07.007

    authors： Boassa D,Lemieux SP,Lev-Ram V,Hu J,Xiong Q,Phan S,Mackey M,Ramachandra R,Peace RE,Adams SR,Ellisman MH,Ngo JT

    更新日期：2019-10-17 00:00:00

  • Proteome-wide Profiling of Clinical PARP Inhibitors Reveals Compound-Specific Secondary Targets.

    abstract：:Poly(ADP-ribose) polymerase (PARP) inhibitors (PARPi) are a promising class of targeted cancer drugs, but their individual target profiles beyond the PARP family, which could result in differential clinical use or toxicity, are unknown. Using an unbiased, mass spectrometry-based chemical proteomics approach, we genera...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2016.10.011

    authors： Knezevic CE,Wright G,Rix LLR,Kim W,Kuenzi BM,Luo Y,Watters JM,Koomen JM,Haura EB,Monteiro AN,Radu C,Lawrence HR,Rix U

    更新日期：2016-12-22 00:00:00

  • Membrane-Active Rhamnolipids Overcome Aminoglycoside Resistance.

    abstract：:In this issue of Cell Chemical Biology, Radlinski et al. (2019) identify Pseudomonas-derived rhamnolipids that potentiate aminoglycoside antibiotics in the eradication of antibiotic-tolerant bacterial phenotypes. Microbial physiological and mechanistic studies indicate that rhamnolipids permeabilize S. aureus membrane...

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2019.09.015

    authors： Yarlagadda V,Wright GD

    更新日期：2019-10-17 00:00:00

  • N-Acetyl Cysteine Functions as a Fast-Acting Antioxidant by Triggering Intracellular H2S and Sulfane Sulfur Production.

    abstract：:The cysteine prodrug N-acetyl cysteine (NAC) is widely used as a pharmacological antioxidant and cytoprotectant. It has been reported to lower endogenous oxidant levels and to protect cells against a wide range of pro-oxidative insults. As NAC itself is a poor scavenger of oxidants, the molecular mechanisms behind the...

    journal_title：Cell chemical biology

    pub_type： 杂志文章

    doi：10.1016/j.chembiol.2018.01.011

    authors： Ezeriņa D,Takano Y,Hanaoka K,Urano Y,Dick TP

    更新日期：2018-04-19 00:00:00

  • Ubiquinone Biosynthetic Complexes in Prokaryotes and Eukaryotes.

    abstract：:Ubiquinone (UQ) is a conserved polyprenylated lipid essential to cellular respiration. Two papers, one in this issue of Cell Chemical Biology (Hajj Chehade et al., 2019) and another in Molecular Cell (Lohman et al., 2019), identify lipid-binding proteins that play crucial roles in chaperoning UQ-intermediates. ...

    journal_title：Cell chemical biology

    pub_type： 评论,杂志文章

    doi：10.1016/j.chembiol.2019.04.005

    authors： Tsui HS,Clarke CF

    更新日期：2019-04-18 00:00:00