Abstract：
:G-quadruplexes are specialized secondary structures in nucleic acids that possess significant conformational polymorphisms. The precise G-quadruplex conformations in vivo and their relevance to biological functions remain controversial and unclear, especially for telomeric G-quadruplexes. Here, we report a novel single-chain variable fragment (scFv) antibody, D1, with high binding selectivity for parallel G-quadruplexes in vitro and in vivo. Genome-wide chromatin immunoprecipitation using D1 and deep-sequencing revealed the consensus sequence for parallel G-quadruplex formation, which is characterized by G-rich sequence with a short loop size (<3 nt). By using D1, telomeric parallel G-quadruplex was identified and its formation was regulated by small molecular ligands targeting and telomere replication. Together, parallel G-quadruplex specific antibody D1 was found to be a valuable tool for determination of G-quadruplex and its conformation, which will prompt further studies on the structure of G-quadruplex and its biological implication in vivo.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
Liu HY,Zhao Q,Zhang TP,Wu Y,Xiong YX,Wang SK,Ge YL,He JH,Lv P,Ou TM,Tan JH,Li D,Gu LQ,Ren J,Zhao Y,Huang ZSdoi
10.1016/j.chembiol.2016.08.013subject
Has Abstractpub_date
2016-10-20 00:00:00pages
1261-1270issue
10eissn
2451-9456issn
2451-9448pii
S2451-9456(16)30303-8journal_volume
23pub_type
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