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Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.

Abstract：

:A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as "multicolor" electron microscopy (EM) via the photooxidation of 3-3'-diaminobenzidine and its derivatives.

journal_name

Cell Chem Biol

journal_title

Cell chemical biology

authors

Boassa D,Lemieux SP,Lev-Ram V,Hu J,Xiong Q,Phan S,Mackey M,Ramachandra R,Peace RE,Adams SR,Ellisman MH,Ngo JT

doi

10.1016/j.chembiol.2019.07.007

subject

Has Abstract

pub_date

2019-10-17 00:00:00

pages

1407-1416.e5

issue

10

eissn

2451-9456

issn

2451-9448

pii

S2451-9456(19)30238-7

journal_volume

26

pub_type

杂志文章

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