Abstract：
:Numerous near-infrared (NIR) fluorescent proteins (FPs) were recently engineered from bacterial photoreceptors but lack of their systematic comparison makes researcher's choice rather difficult. Here we evaluated side-by-side several modern NIR FPs, such as blue-shifted smURFP and miRFP670, and red-shifted mIFP and miRFP703. We found that among all NIR FPs, miRFP670 had the highest fluorescence intensity in various mammalian cells. For instance, in common HeLa cells miRFP703, mIFP, and smURFP were 2-, 9-, and 53-fold dimmer than miRFP670. Either co-expression of heme oxygenase or incubation of cells with heme precursor weakly affected NIR fluorescence, however, in the latter case elevated cellular autofluorescence. Exogenously added chromophore substantially increased smURFP brightness but only slightly enhanced brightness of other NIR FPs. mIFP showed intermediate, while monomeric miRFP670 and miRFP703 exhibited high binding efficiency of endogenous biliverdin chromophore. This feature makes them easy to use as GFP-like proteins for spectral multiplexing with FPs of visible range.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
Shemetov AA,Oliinyk OS,Verkhusha VVdoi
10.1016/j.chembiol.2017.05.018subject
Has Abstractpub_date
2017-06-22 00:00:00pages
758-766.e3issue
6eissn
2451-9456issn
2451-9448pii
S2451-9456(17)30181-2journal_volume
24pub_type
杂志文章abstract：:The cysteine prodrug N-acetyl cysteine (NAC) is widely used as a pharmacological antioxidant and cytoprotectant. It has been reported to lower endogenous oxidant levels and to protect cells against a wide range of pro-oxidative insults. As NAC itself is a poor scavenger of oxidants, the molecular mechanisms behind the...
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
pub_type： 评论,杂志文章
doi：10.1016/j.chembiol.2019.05.012
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2016.10.006
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2019.04.009
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journal_title：Cell chemical biology
pub_type： 杂志文章,评审
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journal_title：Cell chemical biology
pub_type： 评论,杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章,评审
doi：10.1016/j.chembiol.2017.10.011
更新日期：2018-01-18 00:00:00
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journal_title：Cell chemical biology
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doi：10.1016/j.chembiol.2020.05.008
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journal_title：Cell chemical biology
pub_type： 评论,杂志文章
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
pub_type： 评论,杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2017.08.009
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journal_title：Cell chemical biology
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doi：10.1016/j.chembiol.2018.01.009
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journal_title：Cell chemical biology
pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2016.05.017
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abstract：:In this issue of Cell Chemical Biology, Harvey et al. (2020) identify 4E14, a sulfhydryl-containing N-acetyltryptophan analog that selectively disrupts binding to the previously undruggable anti-apoptotic BCL2 paralog BFL1, and elucidate a BFL1 conformational change that facilitates 4E14 interaction. These results pro...
journal_title：Cell chemical biology
pub_type： 杂志文章
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