Abstract：
:The glycan ligands recognized by Siglecs, influenza viruses, and galectins, as well as many plant lectins, are not well defined. To explore their binding to asparagine (Asn)-linked N-glycans, we synthesized a library of isomeric multiantennary N-glycans that vary in terminal non-reducing sialic acid, galactose, and N-acetylglucosamine residues, as well as core fucose. We identified specific recognition of N-glycans by several plant lectins, human galectins, influenza viruses, and Siglecs, and explored the influence of sialic acid linkages and branching of the N-glycans. These results show the unique recognition of complex-type N-glycans by a wide variety of glycan-binding proteins and their abilities to distinguish isomeric structures, which provides new insights into the biological roles of these proteins and the uses of lectins in biological applications to identify glycans.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
Gao C,Hanes MS,Byrd-Leotis LA,Wei M,Jia N,Kardish RJ,McKitrick TR,Steinhauer DA,Cummings RDdoi
10.1016/j.chembiol.2019.01.002subject
Has Abstractpub_date
2019-04-18 00:00:00pages
535-547.e4issue
4eissn
2451-9456issn
2451-9448pii
S2451-9456(19)30002-9journal_volume
26pub_type
杂志文章abstract：:Reactivation of mutant p53 has emerged as a promising approach for cancer therapy. Recent studies have identified several mutant p53-reactivating compounds that target thiol groups in mutant p53. Here we have investigated the relationship between thiol reactivity, p53 thermostabilization, mutant p53 refolding, mutant ...
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