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Discovery of an Unnatural DNA Modification Derived from a Natural Secondary Metabolite.

Abstract：

:Despite widespread interest for understanding how modified bases have evolved their contemporary functions, limited experimental evidence exists for measuring how close an organism is to accidentally creating a new, modified base within the framework of its existing genome. Here, we describe the biochemical and structural basis for how a single-point mutation in E. coli's naturally occurring cytosine methyltransferase can surprisingly endow a neomorphic ability to create the unnatural DNA base, 5-carboxymethylcytosine (5cxmC), in vivo. Mass spectrometry, bacterial genetics, and structure-guided biochemistry reveal this base to be exclusively derived from the natural but sparse secondary metabolite carboxy-S-adenosyl-L-methionine (CxSAM). Our discovery of a new, unnatural DNA modification reveals insights into the substrate selectivity of DNA methyltransferase enzymes, offers a promising new biotechnological tool for the characterization of the mammalian epigenome, and provides an unexpected model for how neomorphic bases could arise in nature from repurposed host metabolites.

journal_name

Cell Chem Biol

journal_title

Cell chemical biology

authors

Wang T,Kohli RM

doi

10.1016/j.chembiol.2020.09.006

subject

Has Abstract

pub_date

2021-01-21 00:00:00

pages

97-104.e4

issue

1

eissn

2451-9456

issn

2451-9448

pii

S2451-9456(20)30378-0

journal_volume

28

pub_type

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