Abstract：
:Interferon-induced transmembrane protein 3 (IFITM3) is a key interferon effector that broadly prevents infection by diverse viruses. However, the cellular factors that control IFITM3 homeostasis and antiviral activity have not been fully elucidated. Using site-specific photo-crosslinking and quantitative proteomic analysis, here we present the identification and functional characterization of VCP/p97 AAA-ATPase as a primary interaction partner of IFITM3. We show that IFITM3 ubiquitination at lysine 24 is crucial for VCP binding, trafficking, turnover, and engagement with incoming virus particles. Consistently, pharmacological inhibition of VCP/p97 ATPase activity leads to defective IFITM3 lysosomal sorting, turnover, and co-trafficking with virus particles. Our results showcase the utility of site-specific protein photo-crosslinking in mammalian cells and reveal VCP/p97 as a key cellular factor involved in IFITM3 trafficking and homeostasis.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
Wu X,Spence JS,Das T,Yuan X,Chen C,Zhang Y,Li Y,Sun Y,Chandran K,Hang HC,Peng Tdoi
10.1016/j.chembiol.2020.03.004subject
Has Abstractpub_date
2020-05-21 00:00:00pages
571-585.e6issue
5eissn
2451-9456issn
2451-9448pii
S2451-9456(20)30078-7journal_volume
27pub_type
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