Abstract：
:Non-ribosomal peptides (NRPs) are biosynthesized on non-ribosomal peptides synthetase (NRPS) complexes, of which a C-terminal releasing domain commonly offloads the products. Interestingly, a dedicated releasing domain is absent in surugamides (SGM) NRPS, which directs the biosynthesis of cyclic octapeptides, SGM-A to -E, and the linear decapeptide, SGM-F. Here, we confirmed that surE is essential for the production of SGMs via genetic experiments. Biochemical characterization demonstrated that the recombinant enzyme, SurE, can generate the main products SGM-A and -F from the corresponding SNAC substrates, indicating that SurE is a standalone thioesterase-like enzyme. SurE also displays considerable substrate plasticity with expanded ring or different amino acid compositions to produce different cyclopeptides, highlighting the potential of chemoenzymatic applications. Site-directed mutagenesis allowed identification of the key residues of SurE. Finally, bioinformatics analysis suggested that SurE homologs are widely distributed in bacteria, suggesting a general mechanism of NRP release in Nature.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
Zhou Y,Lin X,Xu C,Shen Y,Wang SP,Liao H,Li L,Deng H,Lin HWdoi
10.1016/j.chembiol.2019.02.010subject
Has Abstractpub_date
2019-05-16 00:00:00pages
737-744.e4issue
5eissn
2451-9456issn
2451-9448pii
S2451-9456(19)30045-5journal_volume
26pub_type
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journal_title：Cell chemical biology
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