当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > RNA interference influenced the proliferation and invasion of XWLC-05 lung cancer cells through inhibiting aquaporin 3.

RNA interference influenced the proliferation and invasion of XWLC-05 lung cancer cells through inhibiting aquaporin 3.

Abstract：

OBJECTIVE:The objective of this study was to construct a recombinant vector expressing siRNA targetedly inhibiting aquaporin 3 (AQP3), and to evaluate the effects of AQP3 inhibition on the proliferation and invasion of XWLC-05 human lung cancer cells. METHODS:We obtained human AQP3 sequence from the Genbank and established the recombinant vector expressing siRNA targeting AQP3. After the transfection of the recombinant vectors, the expression of AQP3 was determined by RT-PCR and western blot. The MTS assay, flow cytometry and Transwell assay were conducted to detect the proliferation, cell cycle process, apoptosis and invasion of XWLC-05 cells. Then the activity of metal matrix proteinase (MMP) 2 was determined by gelatin zymography. Tumor formation in vivo experiments were also conducted in nude mice. RESULTS:RNA interference (RNAi) of AQP3 substantially suppressed the XWLC-05 cell proliferation and invasion, blocked the cell cycle progressing and promoted cell apoptosis. In addition, the activity of MMP2 was remarkably attenuated in RNAi group. AQP3 RNAi did not affect the tumor formation rate in nude mice but reduced the tumor growth. CONCLUSION:The inhibition of AQP3 retarded the growth and invasiveness of XWLC-05 lung cancer cells and decreased the activity of MMP2.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Xiong G,Chen X,Zhang Q,Fang Y,Chen W,Li C,Zhang J

doi

10.1016/j.bbrc.2017.02.013

subject

Has Abstract

pub_date

2017-04-08 00:00:00

pages

627-634

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)30274-7

journal_volume

485

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • High-affinity interaction between interleukin-11 and S100P protein.

    abstract：:Interleukin-11 (IL-11) and S100P are oncoproteins co-expressed in numerous cancers, which might favor their interaction during oncogenesis. We have explored the possibility of this interaction by surface plasmon resonance spectroscopy, intrinsic fluorescence, and chemical crosslinking. Recombinant forms of IL-11 and S...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.11.024

    authors： Kazakov AS,Sokolov AS,Rastrygina VA,Solovyev VV,Ismailov RG,Mikhailov RV,Ulitin AB,Yakovenko AR,Mirzabekov TA,Permyakov EA,Permyakov SE

    更新日期：2015-12-25 00:00:00

  • Production and secretion of interleukin-1alpha proteins by rat testis.

    abstract：:The present study characterizes constitutively expressed rat testicular interleukin-1alpha (IL-1alpha) proteins. IL-1 bioactivity of crude testis protein was completely neutralized by IL-1alpha antiserum, IL-1 receptor antagonist, and soluble type I IL-1 receptor. Upon non-denaturating gel permeation chromatography, b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02239-8

    authors： Gustafsson K,Sultana T,Zetterström CK,Setchell BP,Siddiqui A,Weber G,Söder O

    更新日期：2002-09-27 00:00:00

  • Kinetic analyses of the effects of hyperoxia and hypoxia on vascular cyclooxygenase activity in vitro.

    abstract：:Kinetic analyses were performed to understand the mechanism of hyperoxic induced inhibition of prostacyclin synthesis by human umbilical arteries. Brief exposure of arterial segments to oxygen resulted in over 30% decrease in Vmax of cyclooxygenase in treated vessels. In contrast, cyclooxygenase from hypoxic arterial ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80350-2

    authors： Yamaja Setty BN,Walenga RW,Stuart MJ

    更新日期：1984-11-30 00:00:00

  • Evidence that post-transcriptional changes in the expression of mitogen regulated protein accompany immortalization of mouse cells.

    abstract：:Primary mouse embryo fibroblasts passed in culture go through a period of declining growth rate and then after a period of minimal proliferation begin to grow again, generating, under appropriate conditions, an immortal 3T3 line. The 3T3 cells, but not the primary mouse embryo fibroblasts, are able to synthesize and t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80144-4

    authors： Edwards DR,Parfett CL,Smith JH,Denhardt DT

    更新日期：1987-08-31 00:00:00

  • Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet.

    abstract：:It has been shown that adipose tissue hyperplasia (increased adipocyte number or adipogenesis) has beneficial effects on metabolic health. The aim of the present study was to determine whether phytol could modulate hyperplasia/adipogenesis and glucose homeostasis, and to explore the underlying mechanisms in mice fed h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.05.160

    authors： Wang J,Hu X,Ai W,Zhang F,Yang K,Wang L,Zhu X,Gao P,Shu G,Jiang Q,Wang S

    更新日期：2017-08-05 00:00:00

  • Ceruloplasmin carries the anionic glycan oligo/poly alpha2,8 deaminoneuraminic acid.

    abstract：:Oligo/poly alpha2,8 deaminoneuraminic acid (KDN), a unique posttranslational protein modification, was found on megalin and a not yet characterized 150 kDa glycoprotein. We purified this glycoprotein from rat testis and identified it as ceruloplasmin. Furthermore, immunoprecipitated ceruloplasmin from rat thymus, ovar...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)00718-0

    authors： Ziak M,Meier M,Novak-Hofer I,Roth J

    更新日期：2002-07-19 00:00:00

  • Changes in autophagy proteins in a rat model of controlled cortical impact induced brain injury.

    abstract：:Autophagy has been implicated in several neurodegenerative diseases and recently its role in acute brain injury has received increased interest. In our study, we investigated the profiles of autophagy-linked proteins (MAP-LC3 (Atg8), beclin-1 (Atg6) and the beclin-1-binding protein, bcl-2, following controlled cortica...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.05.031

    authors： Sadasivan S,Dunn WA Jr,Hayes RL,Wang KK

    更新日期：2008-09-05 00:00:00

  • The ovine CCAAT-enhancer binding protein delta gene: cloning, characterization, and species-specific autoregulation.

    abstract：:Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the regulation of gene expression during growth, differentiation, apoptosis, and inflammation. Autoregulation is relatively common in the modulation of C/EBP gene expression and, for the human and murine C/EBPa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2630

    authors： Davies GE,Sabatakos G,Cryer A,Ramji DP

    更新日期：2000-05-10 00:00:00

  • The follicle promotes the evolution of variants of avian leukosis virus subgroup J in vertical transmission.

    abstract：:Avian leukosis virus (ALV) is one of the main causative agent of tumor development, which brings enormous economic losses to the poultry industry worldwide. ALV can be transmitted horizontally and vertically, and the latter often give rise to more adverse pathogenicity. However, the propagation and evolution of ALV un...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.042

    authors： Gao X,Li Q,Fang L,Song H,Cui Z,Meng F,Zhang Z

    更新日期：2020-01-22 00:00:00

  • Two phosphorylation-independent sites on the p85 SH2 domains bind A-Raf kinase.

    abstract：:Src homology 2 (SH2) domains mediate phosphotyrosine (pY)-dependent protein:protein interactions involved in signal transduction pathways. We have found that the SH2 domains of the 85-kDa alpha subunit (p85) of phosphatidylinositol 3-kinase (PI3 kinase) bind directly to the serine/threonine kinase A-Raf. In this repor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6347

    authors： Fang Y,Johnson LM,Mahon ES,Anderson DH

    更新日期：2002-02-01 00:00:00

  • Identification of FOXE3 transcription factor as a potent oncogenic factor in triple-negative breast cancer.

    abstract：:Triple-negative breast cancer (TNBC) represents a unique subgroup of breast cancers (BCa) with potential to be highly proliferative and invasive. Patient with TNBC are prone to developing resistance to chemotherapy. Therefore, TNBC usually has a poor clinical outcome. The key factors driving these malignant features r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.12.034

    authors： Wang H,Yang T,Yuan Y,Sun X

    更新日期：2020-02-26 00:00:00

  • Unique CCT repeats mediate transcription of the TWIST1 gene in mesenchymal cell lines.

    abstract：:TWIST1, a basic helix-loop-helix transcription factor, plays critical roles in embryo development, cancer metastasis and mesenchymal progenitor differentiation. Little is known about transcriptional regulation of TWIST1 expression. Here we identified DNA sequences responsible for TWIST1 expression in mesenchymal linea...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.114

    authors： Ohkuma M,Funato N,Higashihori N,Murakami M,Ohyama K,Nakamura M

    更新日期：2007-01-26 00:00:00

  • Platelet activation during tumor development, the potential role of BDNF-TrkB autocrine loop.

    abstract：:Platelets are an important place for the storage of angiogenic factors, such as vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor (BDNF). The present study aims to investigate the interaction between BDNF-TrkB pathway and platelet activation during tumor development. In an orthotopic hepa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.06.007

    authors： Yang ZF,Ho DW,Lau CK,Tam KH,Lam CT,Poon RT,Fan ST

    更新日期：2006-08-04 00:00:00

  • Caffeic acid phenethyl ester inhibits 3-MC-induced CYP1A1 expression through induction of hypoxia-inducible factor-1α.

    abstract：:Caffeic acid phenethyl ester (CAPE), a natural component of propolis, is reported to have anticarcinogenic properties, although its precise chemopreventive mechanism remains unclear. In this study, we examined the effects of CAPE on 3-methylcholanthrene (3-MC)-induced CYP1A1 expression and activities. CAPE reduced the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.08.060

    authors： Kim HG,Han EH,Im JH,Lee EJ,Jin SW,Jeong HG

    更新日期：2015-09-25 00:00:00

  • The requirement of yeast Ssl2 (Rad25) for the repair of cisplatin-damaged DNA.

    abstract：:Cisplatin is one of the most widely used anticancer agents. Cisplatin-induced cytotoxicity results from its ability to form cisplatin-DNA adducts within the cellular genome which can inhibit the transcription of genes and the replication of DNA. Cisplatin-adducts are primarily removed by the nucleotide excision repair...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9366

    authors： Yang WL,Cvijic ME,Ishii K,Chin KV

    更新日期：1998-09-29 00:00:00

  • Overexpression of Arabidopsis NADPH-dependent thioredoxin reductase C (AtNTRC) confers freezing and cold shock tolerance to plants.

    abstract：:Overexpression of AtNTRC (AtNTRC(OE)) in Arabidopsis thaliana led to a freezing and cold stress tolerance, whereas a knockout mutant (atntrc) showed a stress-sensitive phenotype. Biochemical analyses showed that the recombinant AtNTRC proteins exhibited a cryoprotective activity for malate dehydrogenase and lactic deh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.06.089

    authors： Moon JC,Lee S,Shin SY,Chae HB,Jung YJ,Jung HS,Lee KO,Lee JR,Lee SY

    更新日期：2015-08-07 00:00:00

  • The DCCD-reactive aspartyl-residue of subunit C from the Escherichia coli ATP-synthase is important for the conformation of F0.

    abstract：:The effect of various point mutations in subunits a and and c of the E. coli ATP-synthase was characterized. In each of the mutants there was no F0-dependent H+-conduction, but still an ATPase-activity comparable to wildtype activities. In addition, the subunit b could be extracted from the mutant's F0 but not from th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91286-5

    authors： Friedl P,Hoppe J,Schairer HU

    更新日期：1984-04-30 00:00:00

  • Sirt3 binds to and deacetylates mitochondrial pyruvate carrier 1 to enhance its activity.

    abstract：:Mitochondrial pyruvate carrier (MPC), composed of MPC1 and MPC2, can modulate pyruvate oxidation in mitochondrial and MPC1 expression correlates with poor prognosis of multiple cancers. Here, we reported that MPC1 is acetylated and its main acetylation sites are: K45 and K46. Sirt3 binds to and deacetylates MPC1. High...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.11.036

    authors： Liang L,Li Q,Huang L,Li D,Li X

    更新日期：2015-12-25 00:00:00

  • Thermodynamic and NMR analyses of NADPH binding to lipocalin-type prostaglandin D synthase.

    abstract：:Lipocalin-type prostaglandin D synthase (L-PGDS) is one of the most abundant proteins in human cerebrospinal fluid (CSF) with dual functions as a prostaglandin D2 (PGD2) synthase and a transporter of lipophilic ligands. Recent studies revealed that L-PGDS plays important roles in protecting against various neuronal di...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.124

    authors： Qin S,Shimamoto S,Maruno T,Kobayashi Y,Kawahara K,Yoshida T,Ohkubo T

    更新日期：2015-12-04 00:00:00

  • Identification of small molecule compounds active against Staphylococcus aureus and Proteus mirabilis.

    abstract：:Staphylococcus aureus is a human pathogen rapidly becoming a serious health problem due to ease of acquiring antibiotic resistance. To help identify potential new drug candidates effective against the pathogen, a small focused library was screened for inhibition of bacterial growth against several pathogens, including...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.10.189

    authors： Swietnicki W,Czarny A,Urbanska N,Drab M

    更新日期：2018-12-02 00:00:00

  • A possible role of cAMP dependent phosphorylation of hepatic microsomal cytochrome P450: a mechanism to increase lipid peroxidation in response to hormone.

    abstract：:Enzymatic lipid peroxidation in hepatocytes is believed to involve cytochrome P450. cAMP dependent phosphorylation of cytochrome P450 was found to increase the NADPH dependent production of malondialdehyde (lipid peroxidation) by about 30%. The cytochrome P450 inhibitor cyanide abolished this activity. The presence of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90878-q

    authors： Mkrtchian SL,Andersson KK

    更新日期：1990-01-30 00:00:00

  • Cell surface trafficking of Fas in NIT-1 cells and dissection of surface and total Fas expression.

    abstract：:The appearance of Fas receptor at the surface of pancreatic beta-cells affected by progressive insulitis strongly suggests that Fas-mediated beta-cell apoptosis plays an important role in the pathogenesis of type 1 diabetes. In support of this concept, the present study has shown that islet cells from NOD mice and the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6215

    authors： Augstein P,Dunger A,Salzsieder C,Heinke P,Kubernath R,Bahr J,Fischer U,Rettig R,Salzsieder E

    更新日期：2002-01-11 00:00:00

  • Ritonavir does not inhibit calpain in vitro.

    abstract：:Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and mu-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.11.161

    authors： Cuerrier D,Nie Z,Badley AD,Davies PL

    更新日期：2005-02-04 00:00:00

  • Mitochondrial calcium dysfunction contributes to autophagic cell death induced by MPP+ via AMPK pathway.

    abstract：:Parkinson's disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Prevailing evidence suggests that abnormal autophagy and mitochondrial dysfunction participate in the process of PD. However, many damages of neuronal functions are regulated b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.148

    authors： Zhao M,Chen J,Mao K,She H,Ren Y,Gui C,Wu X,Zou F,Li W

    更新日期：2019-02-05 00:00:00

  • Spinal miR-34a regulates inflammatory pain by targeting SIRT1 in complete Freund's adjuvant mice.

    abstract：:Sirtuin1 (SIRT1), which is regulated by microRNA-34a (miR-34a), can modulate pathophysiology processes, including nonalcoholic fatty liver disease and intestinal ischemia/reperfusion injury. We previously reported that SIRT1, an NAD+-dependent deacetylase, plays a vital role in the development of neuropathic pain. How...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.07.002

    authors： Chen S,Gu Y,Dai Q,He Y,Wang J

    更新日期：2019-09-03 00:00:00

  • Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant.

    abstract：:A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library. This clone contains a 1521 bp open reading frame coding for a 506 amino acid residue protein which has a 30 amino acid extension peptide which is 6 resi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1792

    authors： Zhou M,Gomez-Sanchez CE

    更新日期：1993-07-15 00:00:00

  • Regulation of ferritin mRNA translation in primary erythroblasts: exogenous c-Kit plus EpoR signaling mimics v-ErbA oncoprotein activity.

    abstract：:In general, translation efficiency of ferritin mRNAs is modulated by variations in iron supply. In primary avian erythroblasts undergoing short-term proliferation, however, ferritin heavy chain (ferH) mRNA is repressed at all iron levels. Yet, expression of v-ErbA oncoprotein is sufficient to reinduce ferH mRNA utiliz...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3300

    authors： Mikulits W,Schranzhofer M,Deiner EM,Beug H,Müllner EW

    更新日期：2000-08-28 00:00:00

  • Increase of estrogen receptor level by thyroxine in estrogen dependent pituitary tumor (MtT/F84) in rats.

    abstract：:A rat transplantable pituitary tumor, MtT/F84, grows much faster in E2 treated rats than in normal females, but is much retarded in thyroidectomized rats. Triiodothyronine (T3) administration in a drinking water increased the tumor growth by the dose dependent manner. The tumor contained both estrogen receptor (ER) an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80677-6

    authors： Fujimoto N,Roy B,Watanabe H,Ito A

    更新日期：1988-04-15 00:00:00

  • In vitro synthesis of a plant phospholipid transfer protein: a study by high performance liquid chromatography.

    abstract：:In order to study the biosynthesis of a plant phospholipid transfer protein (PLTP), poly (A)+RNAs have been prepared from maize seedlings and translated in vitro with a rabbit reticulocyte lysate. The newly synthesized proteins were then separated by fast protein liquid chromatography (FPLC) followed by SDS-PAGE or by...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91843-1

    authors： Tchang F,Laroche-Raynal M,Vergnolle C,Demandre C,Douady D,Grosbois M,Guerbette F,Delseny M,Kader JC

    更新日期：1985-11-27 00:00:00

  • The specificity of the myelin basic protein gene promoter studied in transgenic mice.

    abstract：:The myelin basic proteins (MBPs) are a family of polypeptides that are predominantly expressed in the nervous system where they play a major role in myelination. We have generated four lines of transgenic mice carrying a transgene in which 1.34 kb of the 5'-flanking sequence of the mouse MBP gene was fused upstream of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5837

    authors： Asipu A,Mellor AL,Blair GE

    更新日期：2001-11-09 00:00:00