Podocalyxin as a major pluripotent marker and novel keratan sulfate proteoglycan in human embryonic and induced pluripotent stem cells.

abstract：:Human skin fibroblasts were incubated with a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU), in the presence or absence of tunicamycin. The xyloside-initiated glycosaminoglycans (GAG-MUs) were isolated from the culture medium, and their structures characterized. When the cells were incubated with ...

journal_title：Glycoconjugate journal

authors： Takagaki K,Tazawa T,Munakata H,Nakamura T,Endo M

更新日期：1998-05-01 00:00:00

abstract：:Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-alpha-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1 and RGXT2 in Pi...

journal_title：Glycoconjugate journal

authors： Petersen BL,Egelund J,Damager I,Faber K,Jensen JK,Yang Z,Bennett EP,Scheller HV,Ulvskov P

更新日期：2009-12-01 00:00:00

abstract：:Cultured chondrocytes derived from the caudal and cephalic ends of embryonic chick sterna have been compared with each other and with whole sternum, by using a panel of 21 lectins to probe the distribution of oligosaccharides in glycoconjugates of cells and matrix at various times of culture or development. On culture...

journal_title：Glycoconjugate journal

authors： McClure SF,Stoddart RW,McClure J

更新日期：1997-04-01 00:00:00

abstract：:Over the past two decades, sugar chain structures of human chorionic gonadotropin (hCG) produced in healthy people, three types of trophoblastic disease, and some types of cell lines have been analyzed. The abnormal biantennary structure of hCG is a good marker for the diagnosis of malignant choriocarcinoma. In spite ...

journal_title：Glycoconjugate journal

authors： Takamatsu S,Katsumata T,Inoue N,Watanabe T,Fujibayashi Y,Takeuchi M

更新日期：2004-01-01 00:00:00

abstract：:Influenza virus neuraminidase inhibitors (NAIs), currently used as anti-influenza drugs, can lead to the appearance of drug-resistant variants. Resistance to NAIs appears due to mutations in the active site of the neuraminidase (NA) molecule that decrease the NA enzymatic activity and sometimes in the hemagglutinin (H...

journal_title：Glycoconjugate journal

authors： Mochalova L,Bright R,Xu X,Korchagina E,Chinarev A,Bovin N,Klimov A

更新日期：2010-04-01 00:00:00

abstract：:Twenty different human and animal tissues were investigated for the presence of polyglycosylceramides. The glycolipids were isolated by peracetylation of dry tissue residues left after conventional lipid extraction, followed by extraction with chloroform and subsequent Sephadex LH-20, Sephadex LH-60 and silica gel chr...

journal_title：Glycoconjugate journal

authors： Miller-Podraza H,Stenhagen G,Larsson T,Andersson C,Karlsson KA

更新日期：1997-02-01 00:00:00

abstract：:Synthesis and antigenic reactivity of 6-O-sulfo sialylparagloboside (SPG) and sialyl Lewis X (sLeX) neo-glycolipids containing lactamized neuraminic acid are described. The suitably protected GlcNAc-beta (1-->3)-Gal-beta (1-->4)-GlcOSE derivative was glycosylated with NeuTFAc-alpha (2-->3)-Gal imidate to give NeuTFAc-...

journal_title：Glycoconjugate journal

authors： Yamaguchi M,Ishida H,Kanamori A,Kannagi R,Kiso M

更新日期：2005-03-01 00:00:00

abstract：:To reveal the function of the carbohydrate portion of glycopeptides and glycoproteins, we chemo-enzymatically synthesized artificially N-glycosylated derivatives of eel calcitonin and studied their three-dimensional structure and biological activity. The CD and NMR spectra in trifluoroethanol-H(2)O solution showed tha...

journal_title：Glycoconjugate journal

authors： Tagashira M,Tanaka A,Hisatani K,Isogai Y,Hori M,Takamatsu S,Fujibayashi Y,Yamamoto K,Haneda K,Inazu T,Toma K

更新日期：2001-06-01 00:00:00

abstract：:This review summarizes fifty years of carbohydrate chemistry, from studies of reaction mechanisms and development of synthetic methods to the design of specific compounds for investigations of cancer glycoproteins. Through these years of intensive research, many carbohydrate derivatives were shown to be biologically r...

journal_title：Glycoconjugate journal

authors： Paulsen H,Brockhausen I

更新日期：2001-11-01 00:00:00

abstract：:Alterations in cell membrane glycosylation play important role in oral carcinogenesis. The present study evaluated salivary sialylation changes i.e. total sialic acid (TSA), sialidase activity, linkage specific (α2-3 and α2-6) sialoproteins and sialyl transferase (ST) activity in controls, patients with oral precancer...

journal_title：Glycoconjugate journal

authors： Vajaria BN,Patel KR,Begum R,Patel JB,Shah FD,Joshi GM,Patel PS

更新日期：2014-12-01 00:00:00

abstract：:Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. In this study, we used multiple lectin assays to analyze glycan patterns of serum GP73 and evaluated its diagnostic value for distinguishing hepatocellular carcinoma (HCC) from liver cirrhosis (LC). Firstly, ...

journal_title：Glycoconjugate journal

authors： Jiang K,Shang S,Li W,Guo K,Qin X,Zhang S,Liu Y

更新日期：2015-12-01 00:00:00

abstract：:Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and hFucTVI: Asn46 and Asn91...

journal_title：Glycoconjugate journal

authors： Christensen LL,Bross P,Ørntoft TF

更新日期：2000-12-01 00:00:00

abstract：:A straight forward synthesis of a heptasaccharide part of the cell-wall lipopolysaccharide of Mycobacterium gordonae strain 990, known to have antigenicity, has been achieved in excellent yield. Judicious choice of protecting groups in the intermediates played a significant role throughout the synthesis. Most of the i...

journal_title：Glycoconjugate journal

authors： Panchadhayee R,Kumar Misra A

更新日期：2008-12-01 00:00:00

abstract：:Exposure of neuronal cells to nanomolar concentrations of oligosaccharide portions of ganglioside GM2 and GT1b stimulates cAMP-dependent protein kinase (PKA) Ca2+/calmodulin-dependent protein kinase II (CaMKII), respectively, in a few seconds suggesting the presence of glyco-receptor-like molecules on the surface of t...

journal_title：Glycoconjugate journal

authors： Higashi H,Chen NH

更新日期：2004-01-01 00:00:00

abstract：:Glycation (nonenzymatic glycosylation) in the human lens (cortex and nucleus) in senile (nondiabetic) and diabetic cataracts was studied by measuring the extent of early and late glycation products, the content of free epsilon-amino groups and the formation of disulfide bonds in the soluble lens proteins. There was a ...

journal_title：Glycoconjugate journal

authors： Duhaiman AS

更新日期：1995-10-01 00:00:00

abstract：:Blood group antigen-related oligosaccharides have been implicated in growth regulation, cell mobility control and adhesion; we are therefore interested in the localization of receptors for these oligosaccharides in tumour cells. Labelled neoglycoconjugates that carry synthetic sugar structures are suitable tools to de...

journal_title：Glycoconjugate journal

authors： Kayser K,Bovin NV,Zemlyanukhina TV,Donaldo-Jacinto S,Koopmann J,Gabius HJ

更新日期：1994-08-01 00:00:00

abstract：:The structural diversity and localization of cell surface glycosphingolipids (GSLs), including gangliosides, in glycolipid-enriched microdomains (GEMs, also known as lipid rafts) render them ideally suited to play important roles in mediating intercellular recognition, interactions, adhesion, receptor function, and si...

journal_title：Glycoconjugate journal

authors： Itokazu Y,Tsai YT,Yu RK

更新日期：2017-12-01 00:00:00

abstract：:The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...

journal_title：Glycoconjugate journal

authors： Henry S,Mollicone R,Fernandez P,Samuelsson B,Oriol R,Larson G

更新日期：1996-12-01 00:00:00

abstract：:Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the ...

journal_title：Glycoconjugate journal

authors： Volpi N,Zampini L,Maccari F,Santoro L,Galeotti F,Galeazzi T,Gabrielli O,Coppa GV

更新日期：2013-10-01 00:00:00

abstract：:In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatogr...

journal_title：Glycoconjugate journal

authors： Liu C,Ji HY,Wu P,Yu J,Liu AJ

更新日期：2020-08-01 00:00:00

abstract：:Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A numbe...

journal_title：Glycoconjugate journal

authors： Nemčovičová I,Šesták S,Rendić D,Plšková M,Mucha J,Wilson IB

更新日期：2013-12-01 00:00:00

abstract：:Reductive amination of 3-deoxy-D-manno-octulosonic acid (Kdo) with allylamine (AIIN) or 2-(4-aminophenyl)ethylamine (APEA) yields epimer pairs of 2-N-allylamino and 2-N-[2-(4-aminophenyl)ethylamino]-2,3-dideoxy-D-glycero-D-galacto- and -2,3-dideoxy-D-glycero-D-talo-octonic acid. The yields were 50-60% due to reduction...

journal_title：Glycoconjugate journal

authors： Grimmecke HD,Brade H

更新日期：1998-06-01 00:00:00

abstract：:The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay pro...

journal_title：Glycoconjugate journal

authors： Rizzo AM,Berra B,Rossi F,Guerra A,Gornati R,Bernardini G,Taki T,Kasama T,Mauri L,Sonnino S

更新日期：2002-01-01 00:00:00

abstract：:Colominic acid is an alpha2,8-linked sialic acid polymer produced by Escherichia coli. We found that synthetic sulfated-colominic acids (SC) remarkably inhibited the cytotoxicity of bee and snake venom toward mouse fibroblast cells, but colominic acids showed no inhibition themselves, indicating the important role of ...

journal_title：Glycoconjugate journal

authors： Oda Y,Kinoshita M,Hamada K,Nakayama K,Ohta Y,Yamaguchi S,Tsukada Y,Kawai Y,Kakehi K

更新日期：1999-08-01 00:00:00

abstract：:Full proton NMR assignments have been achieved for the alpha(1-3)-linked fucose residues contained in alkaline borohydride reduced keratan sulphate chains derived from bovine articular cartilage. This involved 500 MHz spectroscopy at 60 degrees C and included COSY and RELAYED-COSY determinations. ...

journal_title：Glycoconjugate journal

authors： Huckerby TN,Nieduszynski IA,Brown GM,Cockin GH

更新日期：1991-02-01 00:00:00

abstract：:Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presen...

journal_title：Glycoconjugate journal

authors： Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Z

更新日期：2019-06-01 00:00:00

abstract：:We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction ...

journal_title：Glycoconjugate journal

authors： Kinsella L,Chen HL,Smith JA,Rudland PS,Fernig DG

更新日期：1998-04-01 00:00:00

abstract：:The sugar chain-binding specificity of tomato lectin (LEA) against glycoproteins was investigated qualitatively using lectin blot analysis. Glycoproteins containing tri- and tetra-antennary complex-type N-glycans were stained with LEA. Unexpectedly, glycoproteins containing high mannose-type N-glycans and a horseradis...

journal_title：Glycoconjugate journal

authors： Oguri S

更新日期：2005-11-01 00:00:00

abstract：:Grifola frondosa is an important fungal research resource. However, there was little report about hyperglycemic activity of Grifola frondosa polysaccharide on insulin resistance in vitro. In this study, the hypoglycemic activity of a polysaccharide obtained from Grifola frondosa (GFP) on HepG2 cell and hpyerglycemic m...

journal_title：Glycoconjugate journal

authors： Ma X,Zhou F,Chen Y,Zhang Y,Hou L,Cao X,Wang C

更新日期：2014-07-01 00:00:00

abstract：:The sap-sucking homopteran insects, commonly known as aphids and leafhoppers are responsible for a huge amount of lost productivity of mustard, chickpea, cabbage, rice and many other important crops. Due to their unique feeding habits and ability to build up a huge population in a very short time, they are very diffic...

journal_title：Glycoconjugate journal

authors： Majumder P,Banerjee S,Das S

更新日期：2004-01-01 00:00:00