Effect of carbohydrate structure on biological activity of artificially N-glycosylated eel calcitonin.

abstract：:Oral exposure to lectins or the presence or absence of bacteria in the rat small intestine were shown by histological methods using anti-lectin antibodies or digoxigenin-labelled lectins to have major effects on the state of glycosylation of lumenal membranes and cytoplasmic glycoconjugates of epithelial cells. Taken ...

journal_title：Glycoconjugate journal

authors： Pusztai A,Ewen SW,Grant G,Peumans WJ,Van Damme EJ,Coates ME,Bardocz S

更新日期：1995-02-01 00:00:00

abstract：:UDP-GlcNAc:Man alpha 1-3R beta 1-2-N-acetylglucosaminyltransferase I (GlcNAc-T I; EC 2.4.1.101) catalyses the conversion of [Man alpha 1-6(Man alpha 1-3)Man alpha 1-6][Man alpha 1-3]Man beta-O-R to [Man alpha 1-6(Man alpha 1-3)Man alpha 1-6] [GlcNAc beta 1-2Man alpha 1-3]Man beta-O-R (R = 1-4GlcNAc beta 1-4GlcNAc- Asn...

journal_title：Glycoconjugate journal

authors： Reck F,Springer M,Meinjohanns E,Paulsen H,Brockhausen I,Schachter H

更新日期：1995-12-01 00:00:00

abstract：:Lubrication of mammalian joints is mediated by lubricin, a product of megakaryocyte stimulating factor gene (MSF; GenBank accession #U70136) expression. Lubricin (M(r) approximately 240 kDa) is a mucinous glycoprotein which is 50% (w/w) post-translationally modified with beta(1-3)Gal-GalNAc incompletely capped with Ne...

journal_title：Glycoconjugate journal

authors： Jay GD,Harris DA,Cha CJ

更新日期：2001-10-01 00:00:00

abstract：:Sialosyl-Le(x) (SLex) and its positional isomer sialosyl-Le(a) are the epitopes recognized by the lectin domain of E- and P-selectins. Expression of SLex in polymorphonuclear leukocytes (PMN) plays an important role in recruitment of these cells at sites of inflammation through activation of selectins. We studied expr...

journal_title：Glycoconjugate journal

authors： Ito K,Handa K,Hakomori S

更新日期：1994-06-01 00:00:00

abstract：:Colominic acid is an alpha2,8-linked sialic acid polymer produced by Escherichia coli. We found that synthetic sulfated-colominic acids (SC) remarkably inhibited the cytotoxicity of bee and snake venom toward mouse fibroblast cells, but colominic acids showed no inhibition themselves, indicating the important role of ...

journal_title：Glycoconjugate journal

authors： Oda Y,Kinoshita M,Hamada K,Nakayama K,Ohta Y,Yamaguchi S,Tsukada Y,Kawai Y,Kakehi K

更新日期：1999-08-01 00:00:00

abstract：:Grifola frondosa is an important fungal research resource. However, there was little report about hyperglycemic activity of Grifola frondosa polysaccharide on insulin resistance in vitro. In this study, the hypoglycemic activity of a polysaccharide obtained from Grifola frondosa (GFP) on HepG2 cell and hpyerglycemic m...

journal_title：Glycoconjugate journal

authors： Ma X,Zhou F,Chen Y,Zhang Y,Hou L,Cao X,Wang C

更新日期：2014-07-01 00:00:00

abstract：:The N-glycans from soluble extracts of ten pollens were examined. The pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase HPLC, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. In compar...

journal_title：Glycoconjugate journal

authors： Wilson IB,Altmann F

更新日期：1998-11-01 00:00:00

abstract：:The conformational space of methyl 6-O-[(R)- and (S)-1-carboxyethyl]-alpha-D-galactopyranoside has been investigated. A grid search employing energy minimization at each grid point over the three major degrees of freedom, namely phi, psi and omega, identified low energy regions. The R-isomer shows five low energy conf...

journal_title：Glycoconjugate journal

authors： Stenutz R,Widmalm G

更新日期：1997-12-01 00:00:00

abstract：:UDP-GlcNAc:alpha3-D-mannoside beta1,2- N -acetylglucosaminyltransferase I (GnTI; EC 2.4.1.101) is a Golgi-resident glycosyltransferase that is essential for the processing of oligomannose to hybrid and complex N-glycans in higher eukaryotes. The cDNA of Nicotiana tabacum GnTI has been cloned and characterised previous...

journal_title：Glycoconjugate journal

authors： Strasser R,Altmann F,Glössl J,Steinkellner H

更新日期：2004-01-01 00:00:00

abstract：:Multidisciplinary approaches by a number of investigators have established that cell-surface carbohydrates are integral components of recognition systems regulating survival, migration, adhesion, growth and differentiation of various cells. Our own experience and contributions to this exciting field are described. We ...

journal_title：Glycoconjugate journal

authors： Muramatsu T

更新日期：2000-07-01 00:00:00

abstract：:As part of our ongoing program aimed at understanding the structural significance of GlcNAcβAsn linkage conserved in all eukaryotic N-glycoproteins, the present study reports on the synthesis and X-ray crystal structures of N-(3-deoxy-3-acetamido-β-D-glycopyranosyl)acetamide (Glc3NAcβNHAc) and the corresponding propio...

journal_title：Glycoconjugate journal

authors： Mathiselvam M,Varghese B,Loganathan D

更新日期：2011-12-01 00:00:00

abstract：:Based on the O-specific polysaccharides of the lipopolysaccharides (O-polysaccharides, O-antigens), strains of a clonal species Escherichia coli are classified into 184 O serogroups. In this work, structures of the O-polysaccharides of E. coli O69 and O146 were elucidated and gene clusters for their biosynthesis were ...

journal_title：Glycoconjugate journal

authors： Knirel YA,Guo X,Senchenkova SN,Perepelov AV,Liu B,Shashkov AS

更新日期：2017-02-01 00:00:00

abstract：:A major impediment in the analysis of glycosaminoglycans is the difficulty to cleave quantitatively the glycosidic bonds because of the stabilisation of glycosidic bonds and of the relative instability of the liberated constituents. This manuscript describes a modified procedure of methanolysis in the presence of bari...

journal_title：Glycoconjugate journal

authors： Zanetta JP,Timmerman P,Leroy Y

更新日期：1999-10-01 00:00:00

abstract：:Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embr...

journal_title：Glycoconjugate journal

authors： Toyoda H,Nagai Y,Kojima A,Kinoshita-Toyoda A

更新日期：2017-04-01 00:00:00

abstract：:We have previously shown that murine resident peritoneal macrophages (PEMs) are activated in response to uptake of oligomannose-coated liposomes (OMLs), leading to production of interleukin (IL)-12. To understand the mechanism of activation of PEMs by OMLs, in the present study we investigated the role of a mannose-bi...

journal_title：Glycoconjugate journal

authors： Kato C,Kojima N

更新日期：2010-07-01 00:00:00

abstract：:Mammalian sulfoglycolipids are comprised of two major members, sulfatide (SO(3)-3Gal-ceramide) and seminolipid (SO(3)-3Gal-alkylacylglycerol). Sulfatide is abundant in the myelin sheath and seminolipid is expressed on the spermatogenic cells. Cerebroside sulfotransferase (CST)-deficient mice generated by gene targetin...

journal_title：Glycoconjugate journal

authors： Honke K,Zhang Y,Cheng X,Kotani N,Taniguchi N

更新日期：2004-01-01 00:00:00

abstract：:To elucidate the mechanism underlying the hydrolysis of the GalNAcbeta1-->4Gal linkage in ganglioside GM2 [GalNAcbeta1-->4(NeuAcalpha2-->3)Galbeta1-->4Glcbeta1-->1' Cer] by beta-hexosaminidase A (Hex A) with GM2 activator protein, we designed and synthesized two kinds of GM2 linkage analogues-6'-NeuAc-GM2 and alpha-Ga...

journal_title：Glycoconjugate journal

authors： Komori T,Ando T,Imamura A,Li YT,Ishida H,Kiso M

更新日期：2008-10-01 00:00:00

abstract：:Heteropolysaccarides were isolated from the Korean medicinal plant, Phellodendri cortex (Hwangbek), by hot water and alkali extractions. The extracted polysaccharides were fractionated into eight fractions and they are mainly composed of D-N-acetylglucosamine, D-galactose, D-mannose, and D-glucose. Among the polysacch...

journal_title：Glycoconjugate journal

authors： Park JI,Shim JK,Do JW,Kim SY,Seo EK,Kwon HJ,Lee TK,Kim JK,Choi DY,Kim CH

更新日期：1999-03-01 00:00:00

abstract：:Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20-40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but research is in progress to...

journal_title：Glycoconjugate journal

authors： Maras M,van Die I,Contreras R,van den Hondel CA

更新日期：1999-02-01 00:00:00

abstract：:Recently, we demonstrated that the human xylosyltransferase II (XT-II) has enzymatic activity and is able to catalyze the initial and rate-limiting step in the biosynthesis of glycosaminoglycans (GAGs) like chondroitin and dermatan sulfate, as well as heparan sulfate and heparin. Therefore, this enzyme also very likel...

journal_title：Glycoconjugate journal

authors： Müller B,Prante C,Knabbe C,Kleesiek K,Götting C

更新日期：2013-04-01 00:00:00

abstract：:A gene encoding a putative sialidase was identified in the genome of the opportunistic fungal pathogen, Aspergillus fumigatus. Computational analysis showed that this protein has Asp box and FRIP domains, it was predicted to have an extracellular localization, and a mass of 42 kDa, all of which are characteristics of ...

journal_title：Glycoconjugate journal

authors： Warwas ML,Yeung JH,Indurugalla D,Mooers AO,Bennet AJ,Moore MM

更新日期：2010-07-01 00:00:00

abstract：:O-Glycopyranosyl-N-hydroxysuccinimides of glucose and lactose have been synthesized. Peracetylated sugars, acetobromo sugars and benzoylated thioethyl glycosides have been used as precursors. With boron trifluoride etherate in dichloromethane, peracetylated sugars gave predominantly the beta anomer. Trimethylsilyl tri...

journal_title：Glycoconjugate journal

authors： Andersson M,Oscarson S

更新日期：1992-06-01 00:00:00

abstract：:Transmannosylation from mannotriose (Man beta 1-4Man beta 1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue of N,N'-diacetylchitobiose was regioselectively induced through the use of beta-D-mannanase from Aspergillus niger. The enzyme formed the trisaccharide Man beta 1-4GlcNAc beta 1-4GlcN...

journal_title：Glycoconjugate journal

authors： Usui T,Suzuki M,Sato T,Kawagishi H,Adachi K,Sano H

更新日期：1994-04-01 00:00:00

abstract：:The modulation of GnT-V activity by signaling molecules in PI-3-K/PKB pathway in human hepatocarcinoma cell line 7721 was studied. GnT-V activity was determined after the transfection of sense or antisense cDNA of PKB into the cells, as well as the addition of activators, specific inhibitors, and the antibodies to the...

journal_title：Glycoconjugate journal

authors： Guo HB,Shen ZH,Huang CX,Ma J,Huang Y,Chen HL

更新日期：2000-05-01 00:00:00

abstract：:Glycation (nonenzymatic glycosylation) in the human lens (cortex and nucleus) in senile (nondiabetic) and diabetic cataracts was studied by measuring the extent of early and late glycation products, the content of free epsilon-amino groups and the formation of disulfide bonds in the soluble lens proteins. There was a ...

journal_title：Glycoconjugate journal

authors： Duhaiman AS

更新日期：1995-10-01 00:00:00

abstract：:Amphipathic sulpholipids have been shown to inhibit pancreatic serine proteases due to their detergent-like properties. To evaluate the structural requirement for this inhibitory activity, we examined the effects of various derivatives of sulphoglycolipids, some of which were prepared by deacylation with sphingolipid ...

journal_title：Glycoconjugate journal

authors： Suzuki H,Ito M,Kimura T,Iwamori Y,Iwamori M

更新日期：2000-11-01 00:00:00

abstract：:Trichomonas vaginalis causes the most common non-viral sexually transmitted infection linked to increased risk of premature birth, cervical cancer and HIV. This study defines molecular domains of the parasite surface glycoconjugate lipophosphoglycan (LPG) with distinct functions in the host immunoinflammatory response...

journal_title：Glycoconjugate journal

authors： Singh BN,Hayes GR,Lucas JJ,Sommer U,Viseux N,Mirgorodskaya E,Trifonova RT,Sassi RR,Costello CE,Fichorova RN

更新日期：2009-01-01 00:00:00

abstract：:Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...

journal_title：Glycoconjugate journal

authors： Mandal C,Srinivasan GV,Chowdhury S,Chandra S,Mandal C,Schauer R,Mandal C

更新日期：2009-01-01 00:00:00

abstract：:Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and hFucTVI: Asn46 and Asn91...

journal_title：Glycoconjugate journal

authors： Christensen LL,Bross P,Ørntoft TF

更新日期：2000-12-01 00:00:00

abstract：:In aging and diabetes, glycation of collagen molecules leads to the formation of cross-links that could alter the surface charge on collagen fibrils, and hence affect the properties and correct functioning of a number of tissues. The electron-optical stain phosphotungstic acid (PTA) binds to positively charged amino a...

journal_title：Glycoconjugate journal

authors： Hadley JC,Meek KM,Malik NS

更新日期：1998-08-01 00:00:00