Modulation of the basal activity of phosphatidylinositol-3-kinase/protein kinase B signaling pathway in human hepatocarcinoma cells.

abstract：:The human glioma D-54MG cell line grown in vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3'-isoLM1, an alteration that was do...

journal_title：Glycoconjugate journal

authors： Fredman P,Wikstrand CJ,Månsson JE,Reifenberger G,Bigner SH,Rasheed A,Svennerholm L,Bigner DD

更新日期：1996-06-01 00:00:00

abstract：:In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatogr...

journal_title：Glycoconjugate journal

authors： Liu C,Ji HY,Wu P,Yu J,Liu AJ

更新日期：2020-08-01 00:00:00

abstract：:Syndecan-4 is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family. Syndecan-4-deficient [(Synd4(-/-)] mice were produced to clarify the in vivo role of syndecan-4. Synd4(-/-) mice were more susceptible to kappa-carrageenan-induced nephropathy, and the placental labyrinth from the deficient em...

journal_title：Glycoconjugate journal

authors： Ishiguro K,Kojima T,Muramatsu T

更新日期：2002-05-01 00:00:00

abstract：:Using a focused glycan-gene microarray, we compared the glycosyltransferase (GT) and sulfotransferase gene expression profiles of human monocytes, dendritic cells (DCs) and macrophages (Mphis), isolated or differentiated from the same donors. Microarray analysis indicated that monocytes express transcripts for a full ...

journal_title：Glycoconjugate journal

authors： Trottein F,Schaffer L,Ivanov S,Paget C,Vendeville C,Cazet A,Groux-Degroote S,Lee S,Krzewinski-Recchi MA,Faveeuw C,Head SR,Gosset P,Delannoy P

更新日期：2009-12-01 00:00:00

abstract：:The partitioning of the overall molecular charge distribution into atom centered monopole charges, while quantum mechanically ill-defined, is nevertheless a technique which finds applications in several broad classes of chemical problems. Charges derived from fits to electrostatic potentials have an intuitive appeal s...

journal_title：Glycoconjugate journal

authors： Carey C,Chirlian LE,Francl MM,Gange DM

更新日期：1997-06-01 00:00:00

abstract：:In this study, the content, structure and residual percentages of glycosaminoglycans (GAGs) in the feces of seven breastfed newborns after ingesting a known amount of milk were studied. A comparison was made with five newborns fed with formula milk. Characterization of GAGs from milk and feces samples was performed ac...

journal_title：Glycoconjugate journal

authors： Maccari F,Mantovani V,Gabrielli O,Carlucci A,Zampini L,Galeazzi T,Galeotti F,Coppa GV,Volpi N

更新日期：2016-04-01 00:00:00

abstract：:Human Gb3/CD77 synthase (α1,4-galactosyltransferase) is the only known glycosyltransferase that changes acceptor specificity because of a point mutation. The enzyme, encoded by A4GALT locus, is responsible for biosynthesis of Gal(α1-4)Gal moiety in Gb3 (CD77, Pk antigen) and P1 glycosphingolipids. We showed before tha...

journal_title：Glycoconjugate journal

authors： Kaczmarek R,Mikolajewicz K,Szymczak K,Duk M,Majorczyk E,Krop-Watorek A,Buczkowska A,Czerwinski M

更新日期：2016-12-01 00:00:00

abstract：:Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...

journal_title：Glycoconjugate journal

authors： Mandal C,Srinivasan GV,Chowdhury S,Chandra S,Mandal C,Schauer R,Mandal C

更新日期：2009-01-01 00:00:00

abstract：:Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. In this study, we used multiple lectin assays to analyze glycan patterns of serum GP73 and evaluated its diagnostic value for distinguishing hepatocellular carcinoma (HCC) from liver cirrhosis (LC). Firstly, ...

journal_title：Glycoconjugate journal

authors： Jiang K,Shang S,Li W,Guo K,Qin X,Zhang S,Liu Y

更新日期：2015-12-01 00:00:00

abstract：:Fractions were prepared from the water-soluble components of Aspergillus fumigatus mycelium either by lectin-affinity chromatography or salt precipitation. While they varied considerably in their amino-acid composition, each contained a preponderance of aspartic and glutamic acids. 13C-NMR spectroscopy of these fracti...

journal_title：Glycoconjugate journal

authors： Hearn VM,Griffiths BL,Gorin PA

更新日期：1989-01-01 00:00:00

abstract：:Heterologous complementation in yeast has been a successful tool for cloning and characterisation of genes from various organisms. Therefore we constructed conditionally lethal Saccharomyces cerevisiae strains by replacing the endogenous promoter from the genes of interest (glycosyltransferases) by the stringently reg...

journal_title：Glycoconjugate journal

authors： Mazhari-Tabrizi R,Blank M,Mumberg D,Funk M,Schwarz RT,Eckert V

更新日期：1999-11-01 00:00:00

abstract：:Alterations in cell membrane glycosylation play important role in oral carcinogenesis. The present study evaluated salivary sialylation changes i.e. total sialic acid (TSA), sialidase activity, linkage specific (α2-3 and α2-6) sialoproteins and sialyl transferase (ST) activity in controls, patients with oral precancer...

journal_title：Glycoconjugate journal

authors： Vajaria BN,Patel KR,Begum R,Patel JB,Shah FD,Joshi GM,Patel PS

更新日期：2014-12-01 00:00:00

abstract：:Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embr...

journal_title：Glycoconjugate journal

authors： Toyoda H,Nagai Y,Kojima A,Kinoshita-Toyoda A

更新日期：2017-04-01 00:00:00

abstract：:The IgG Fc glycans strongly influence the Fcγ receptor interactions and Fc-mediated effector mechanisms. Changes in the structure of IgG glycans are associated with various diseases, such as infections and autoimmunity. However, the possible role of Fc glycans in tumor immunity is not yet fully understood. The aim of ...

journal_title：Glycoconjugate journal

authors： Kodar K,Stadlmann J,Klaamas K,Sergeyev B,Kurtenkov O

更新日期：2012-01-01 00:00:00

abstract：:Galectins are animal lectins that can specifically bind beta-galactosides. Thirteen galectins have already been described. This review focuses on a specific member of this family: galectin-8. This galectin was discovered in prostate cancer cells eight years ago and has been studied extensively in the last few years. T...

journal_title：Glycoconjugate journal

authors： Bidon-Wagner N,Le Pennec JP

更新日期：2002-01-01 00:00:00

abstract：:In this study, the antibody 3G9-A was assayed for activity against human erythrocyte glycosphingolipids. The antibody was found to recognize glycosphingolipid components from blood group A erythrocytes but not glycosphingolipids from blood group B or O erythrocytes. Subsequent investigation revealed that the glycosphi...

journal_title：Glycoconjugate journal

authors： Briggs JB,Mandrell R,Macher BA

更新日期：1996-06-01 00:00:00

abstract：:Non-acid and acid glycosphingolipids were isolated from feces of one litter of germ-free rats from day 17 to day 51. Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384-89] were also found in the feces of these germ...

journal_title：Glycoconjugate journal

authors： Larson G,Midtvedt T

更新日期：1989-01-01 00:00:00

abstract：:RNA from four colorectal carcinoma cell lines was prepared and analysed in Northern blots using probes for the MUC2, MUC4, and MUC5AC mucin apoprotein genes. The sizes of the transcripts were very large, in the order of at least 12-16 kb. The presence of distinct bands is in contrast to earlier reports, where these tr...

journal_title：Glycoconjugate journal

authors： Baeckström D,Hansson GC

更新日期：1996-10-01 00:00:00

abstract：:In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45...

journal_title：Glycoconjugate journal

authors： Hernández P,Debray H,Jaekel H,Garfias Y,Jiménez Md Mdel C,Martínez-Cairo S,Zenteno E

更新日期：2001-04-01 00:00:00

abstract：:The N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J 5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoprotei...

journal_title：Glycoconjugate journal

authors： Altmann F

更新日期：1998-01-01 00:00:00

abstract：:The specificity of the sialic acid-binding lectin from ovine placenta was examined in detail by haemagglutination inhibition assays applying a panel of 32 synthetic sialic acid analogues. The carboxylic acid group is a prerequisite for the interaction with the lectin, the alpha-anomer of the methyl glycoside is only a...

journal_title：Glycoconjugate journal

authors： Troncoso MF,Iglesias MM,Isecke R,Todel CW,Brossmer R

更新日期：2000-10-01 00:00:00

abstract：:Five monoclonal antibodies AS17, 22, 25, 38 and 48, a single monoclonal antibody ACH55, and three monoclonal antibodies NAH33, 43, 46, that recognize acharan sulfate (IdoA2S-GlcNAc)n, acharan (IdoA-GlcNAc)n and N-acetyl-heparosan (GlcA-GlcNAc)n, respectively, were generated by immunization of mice with keyhole limpet ...

journal_title：Glycoconjugate journal

authors： Suzuki K,Yamamoto K,Kariya Y,Maeda H,Ishimaru T,Miyaura S,Fujii M,Yusa A,Joo EJ,Kimata K,Kannagi R,Kim YS,Kyogashima M

更新日期：2008-11-01 00:00:00

abstract：:Based on the O-specific polysaccharides of the lipopolysaccharides (O-polysaccharides, O-antigens), strains of a clonal species Escherichia coli are classified into 184 O serogroups. In this work, structures of the O-polysaccharides of E. coli O69 and O146 were elucidated and gene clusters for their biosynthesis were ...

journal_title：Glycoconjugate journal

authors： Knirel YA,Guo X,Senchenkova SN,Perepelov AV,Liu B,Shashkov AS

更新日期：2017-02-01 00:00:00

abstract：:A straight forward synthesis of a heptasaccharide part of the cell-wall lipopolysaccharide of Mycobacterium gordonae strain 990, known to have antigenicity, has been achieved in excellent yield. Judicious choice of protecting groups in the intermediates played a significant role throughout the synthesis. Most of the i...

journal_title：Glycoconjugate journal

authors： Panchadhayee R,Kumar Misra A

更新日期：2008-12-01 00:00:00

abstract：:Coarse tea is made of mature tea plant (Camellia sinensis L.) shoots and is generally discarded as a worthless crop product, but has been proved an excellent material for the treatment of diabetes. This study aims to evaluate the effects of the extraction techniques WE (water extraction), UAE (ultrasound-assisted extr...

journal_title：Glycoconjugate journal

authors： Zhu J,Chen Z,Zhou H,Yu C,Han Z,Shao S,Hu X,Wei X,Wang Y

更新日期：2020-04-01 00:00:00

abstract：:Egg yolk, a large proportion of the egg, was studied for the preparation of N-acetylneuraminic acid (Neu5Ac). The delipidated hen egg yolk (DEY; 500 kg containing 0.2% w/w, Neu5Ac) was hydrolysed with HCl (pH 1.4) at 80 degrees C and neutralized with NaOH (pH 6.0). The mixture was filtered and electrodialysed until th...

journal_title：Glycoconjugate journal

authors： Koketsu M,Juneja LR,Kawanami H,Kim M,Yamamoto T

更新日期：1992-04-01 00:00:00

abstract：:UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II (GnT II; EC 2.4.1.143) is a medial-Golgi resident enzyme that catalyses an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides. Screening a cDNA library from Xenopus laevis ovary with a human ...

journal_title：Glycoconjugate journal

authors： Mucha J,Svoboda B,Kappel S,Strasser R,Bencur P,Fröhwein U,Schachter H,Mach L,Glössl J

更新日期：2002-03-01 00:00:00

abstract：:The partial oligosaccharide sequences of glycoconjugates and the nature of their glycosidic linkages were investigated in normal human prostate, benign prostatic hyperplasia (BPH) and prostatic carcinoma by means of lectin histochemistry, using light microscopy and Western blot analysis. The labeling pattern of BPH di...

journal_title：Glycoconjugate journal

authors： Arenas MI,Romo E,de Gaspar I,de Bethencourt FR,Sánchez-Chapado M,Fraile B,Paniagua R

更新日期：1999-07-01 00:00:00

abstract：:Cultured chondrocytes derived from the caudal and cephalic ends of embryonic chick sterna have been compared with each other and with whole sternum, by using a panel of 21 lectins to probe the distribution of oligosaccharides in glycoconjugates of cells and matrix at various times of culture or development. On culture...

journal_title：Glycoconjugate journal

authors： McClure SF,Stoddart RW,McClure J

更新日期：1997-04-01 00:00:00

abstract：:Exposure of neuronal cells to nanomolar concentrations of oligosaccharide portions of ganglioside GM2 and GT1b stimulates cAMP-dependent protein kinase (PKA) Ca2+/calmodulin-dependent protein kinase II (CaMKII), respectively, in a few seconds suggesting the presence of glyco-receptor-like molecules on the surface of t...

journal_title：Glycoconjugate journal

authors： Higashi H,Chen NH

更新日期：2004-01-01 00:00:00