Abstract:
:To elucidate the mechanism underlying the hydrolysis of the GalNAcbeta1-->4Gal linkage in ganglioside GM2 [GalNAcbeta1-->4(NeuAcalpha2-->3)Galbeta1-->4Glcbeta1-->1' Cer] by beta-hexosaminidase A (Hex A) with GM2 activator protein, we designed and synthesized two kinds of GM2 linkage analogues-6'-NeuAc-GM2 and alpha-GalNAc-GM2. In this paper, the efficient and systematic synthesis of these GM2 analogues was described. The highlight of our synthesis process is that the key intermediates, newly developed sialyllactose derivatives, were efficiently prepared in sufficient quantities; these derivatives directly served as highly reactive glycosyl acceptors and coupled with GalNTroc donors to furnish the assembly of GM2 tetrasaccharides in large quantities.
journal_name
Glycoconj Jjournal_title
Glycoconjugate journalauthors
Komori T,Ando T,Imamura A,Li YT,Ishida H,Kiso Mdoi
10.1007/s10719-008-9117-9subject
Has Abstractpub_date
2008-10-01 00:00:00pages
647-61issue
7eissn
0282-0080issn
1573-4986journal_volume
25pub_type
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