Early stage fetal neocortex exhibits a complex ganglioside profile as revealed by high resolution tandem mass spectrometry.

abstract：:UDP-2-(2-ketopropyl)galactose (1) has been utilized as a valuable probe for profiling proteins modified by O-GlcNAc. In this work, we developed a protocol for efficient synthesis of 1. Thus, 2-methallylgalactose derivative 11, a synthetic intermediate for the compound 1, was prepared by stereoselective iodination and ...

journal_title：Glycoconjugate journal

authors： Sakamoto Y,Ohta T,Ito Y

更新日期：2015-10-01 00:00:00

abstract：:A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose, N-acetyl glucosamine and N-acetyl neuraminic ...

journal_title：Glycoconjugate journal

authors： Aparna HS,Salimath PV

更新日期：1996-02-01 00:00:00

abstract：:C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excret...

journal_title：Glycoconjugate journal

authors： Hossain TJ,Manabe S,Ito Y,Iida T,Kosono S,Ueda K,Hosomi A,Inoue D,Suzuki T

更新日期：2018-04-01 00:00:00

abstract：:GM3 ganglioside interacts specifically with complex-type N-linked glycans having multivalent GlcNAc termini, as shown for (1) and (2) below. (1) Oligosaccharides (OS) isolated from ConA-non-binding N-linked glycans of ovalbumin, whose structures were identified as penta-antennary complex-type with bisecting GlcNAc, ha...

journal_title：Glycoconjugate journal

authors： Yoon SJ,Nakayama K,Takahashi N,Yagi H,Utkina N,Wang HY,Kato K,Sadilek M,Hakomori SI

更新日期：2006-12-01 00:00:00

abstract：:Maackia amurensis haemagglutinin (MAH) is a leguminous lectin which preferentially binds to a cluster of sialylated O-linked carbohydrate chains (Konami Y, Yamamoto K. Osawa T, Irimura T (1994) FEBS Lett 342:334-38). In the present study a 950 bp cDNA clone encoding MAH was isolated from a cDNA library constructed fro...

journal_title：Glycoconjugate journal

authors： Yamamoto K,Ishida C,Saito M,Konami Y,Osawa T,Irimura T

更新日期：1994-12-01 00:00:00

abstract：:Heteropolysaccarides were isolated from the Korean medicinal plant, Phellodendri cortex (Hwangbek), by hot water and alkali extractions. The extracted polysaccharides were fractionated into eight fractions and they are mainly composed of D-N-acetylglucosamine, D-galactose, D-mannose, and D-glucose. Among the polysacch...

journal_title：Glycoconjugate journal

authors： Park JI,Shim JK,Do JW,Kim SY,Seo EK,Kwon HJ,Lee TK,Kim JK,Choi DY,Kim CH

更新日期：1999-03-01 00:00:00

abstract：:O-Glycopyranosyl-N-hydroxysuccinimides of glucose and lactose have been synthesized. Peracetylated sugars, acetobromo sugars and benzoylated thioethyl glycosides have been used as precursors. With boron trifluoride etherate in dichloromethane, peracetylated sugars gave predominantly the beta anomer. Trimethylsilyl tri...

journal_title：Glycoconjugate journal

authors： Andersson M,Oscarson S

更新日期：1992-06-01 00:00:00

abstract：:Humans, in contrast to other mammals, do not synthesize N-glycolyl-neuraminic acid (Neu5Gc) due to a deletion in the gene (cmah) encoding the enzyme responsible for this conversion, the cytidine monophospho-N-acetyl-neuraminic acid hydroxylase (CMP-Neu5Ac hydroxylase). The detection of considerable amounts of Neu5Gc-s...

journal_title：Glycoconjugate journal

authors： Casadesús AV,Fernández-Marrero Y,Clavell M,Gómez JA,Hernández T,Moreno E,López-Requena A

更新日期：2013-10-01 00:00:00

abstract：:Grifola frondosa is an important fungal research resource. However, there was little report about hyperglycemic activity of Grifola frondosa polysaccharide on insulin resistance in vitro. In this study, the hypoglycemic activity of a polysaccharide obtained from Grifola frondosa (GFP) on HepG2 cell and hpyerglycemic m...

journal_title：Glycoconjugate journal

authors： Ma X,Zhou F,Chen Y,Zhang Y,Hou L,Cao X,Wang C

更新日期：2014-07-01 00:00:00

abstract：:This review discusses the challenges facing research in 'functional glycomics' and the novel technologies that are being developed to advance the field. The structural complexity of glycans and glycoconjugates makes studies of both their structures and recognition difficult. However, these intricate structures can be ...

journal_title：Glycoconjugate journal

authors： Song X,Heimburg-Molinaro J,Smith DF,Cummings RD

更新日期：2015-10-01 00:00:00

abstract：:In this study, the antibody 3G9-A was assayed for activity against human erythrocyte glycosphingolipids. The antibody was found to recognize glycosphingolipid components from blood group A erythrocytes but not glycosphingolipids from blood group B or O erythrocytes. Subsequent investigation revealed that the glycosphi...

journal_title：Glycoconjugate journal

authors： Briggs JB,Mandrell R,Macher BA

更新日期：1996-06-01 00:00:00

abstract：:Non-acid and acid glycosphingolipids were isolated from feces of one litter of germ-free rats from day 17 to day 51. Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384-89] were also found in the feces of these germ...

journal_title：Glycoconjugate journal

authors： Larson G,Midtvedt T

更新日期：1989-01-01 00:00:00

abstract：:We have previously detected an alkali-labile and developmentally regulated antigen in rat embryonic cerebral cortex, which may be 9-O-acetylsialylated GT3 ganglioside (Hirabayashi Y, Hirota M, Suzuki Y, Matsumoto M, Obata K, Ando S (1989) Neurosci Lett 106:193-98). In this study we established a mouse monoclonal antib...

journal_title：Glycoconjugate journal

authors： Zhang G,Ji L,Kurono S,Fujita SC,Furuya S,Hirabayashi Y

更新日期：1997-11-01 00:00:00

abstract：:Synthetic thioglycoside-analogs of gangliosides such as Neu5Ac alpha(2-S-6)Glc beta(1-1)Ceramide (1) and the GM3 analog Neu5Ac alpha(2-S-6)Gal beta(1-4)Glc beta(1-1)Ceramide (2), competitively inhibited GM3 hydrolysis by the sialidase of different subtypes of human and animal influenza viruses with an apparent Ki valu...

journal_title：Glycoconjugate journal

authors： Suzuki Y,Sato K,Kiso M,Hasegawa A

更新日期：1990-01-01 00:00:00

abstract：:Carbohydrate-carbohydrate interaction is a reliable and versatile mechanism for cell adhesion and recognition. Glycosphingolipid (GSL) clusters at the cell membrane are mainly involved in this interaction. To investigate carbohydrate-carbohydrate interaction an integrated strategy (Glyconanotechnology) was developed. ...

journal_title：Glycoconjugate journal

authors： de la Fuente JM,Penadés S

更新日期：2004-01-01 00:00:00

abstract：:Infectious disease, commonly caused by bacterial pathogens, is now the world's leading cause of premature death and third overall cause behind cardiovascular disease and cancer. Urinary Tract Infection (UTI), caused by E. coli bacteria, is a very common bacterial infection, a majority in women (85%) and may result in ...

journal_title：Glycoconjugate journal

authors： Basu M,Seggerson S,Henshaw J,Jiang J,del A Cordona R,Lefave C,Boyle PJ,Miller A,Pugia M,Basu S

更新日期：2004-01-01 00:00:00

abstract：:Abnormal glycosylation of proteins is known to be either resultant or causative of a variety of diseases. This makes glycoproteins appealing targets as potential biomarkers and focal points of molecular studies on the development and progression of human ailment. To date, a majority of efforts in disease glycoproteomi...

journal_title：Glycoconjugate journal

authors： Schumacher KN,Dodds ED

更新日期：2016-06-01 00:00:00

abstract：:The mechanism of interleukin-3 (IL-3) independent cell growth and of IL-3-associated ganglioside expression was analysed using the IL-3 dependent murine myelogenous leukaemia cell line NFS60-I7 and IL-3 gene-transfected sublines. The transfected cell lines showed autonomous cell growth, tumorigenicity, and IL-3 associ...

journal_title：Glycoconjugate journal

authors： Nakamura M,Kirito K,Tsunoda A,Hara K,Furukawa Y,Saito M

更新日期：1996-04-01 00:00:00

abstract：:Reductive amination of 3-deoxy-D-manno-octulosonic acid (Kdo) with allylamine (AIIN) or 2-(4-aminophenyl)ethylamine (APEA) yields epimer pairs of 2-N-allylamino and 2-N-[2-(4-aminophenyl)ethylamino]-2,3-dideoxy-D-glycero-D-galacto- and -2,3-dideoxy-D-glycero-D-talo-octonic acid. The yields were 50-60% due to reduction...

journal_title：Glycoconjugate journal

authors： Grimmecke HD,Brade H

更新日期：1998-06-01 00:00:00

abstract：:A group of fluorescent statistical glycopolymers, prepared via reversible addition-fragmentation chain-transfer (RAFT)-based polymerizations, were successfully employed in lectin-mediated bacterial binding studies. The resultant glycopolymers contained three different monomers: N-(2-hydroxyethyl) acrylamide (HEAA), N-...

journal_title：Glycoconjugate journal

authors： Wang W,Chance DL,Mossine VV,Mawhinney TP

更新日期：2014-02-01 00:00:00

abstract：:Our recent studies have revealed the existence of two distinct Gal: 3-O-sulfotransferases capable of acting on the C-3 position of galactose in a Core 2 branched structure, e.g., Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNacalph a1-->OBenzyl as acceptor to give 3-O-sulfoGalbeta1-->4GlcNAcbeta1-->3(Galbeta1-->3)G alN...

journal_title：Glycoconjugate journal

authors： Jain RK,Piskorz CF,Chandrasekaran EV,Matta KL

更新日期：1998-10-01 00:00:00

abstract：:Over the last few years enormous interest has been shown in the structures of the glycan moieties of various parasite surface glycoconjugates. Structures have been determined for the glyco-components of glycosylphosphatidylinositol (GPI) protein membrane anchors, for asparagine-linked oligosaccharides, and for the gly...

journal_title：Glycoconjugate journal

authors： Zamze S

更新日期：1991-12-01 00:00:00

abstract：:This review summarizes fifty years of carbohydrate chemistry, from studies of reaction mechanisms and development of synthetic methods to the design of specific compounds for investigations of cancer glycoproteins. Through these years of intensive research, many carbohydrate derivatives were shown to be biologically r...

journal_title：Glycoconjugate journal

authors： Paulsen H,Brockhausen I

更新日期：2001-11-01 00:00:00

abstract：:As part of our ongoing program aimed at understanding the structural significance of GlcNAcβAsn linkage conserved in all eukaryotic N-glycoproteins, the present study reports on the synthesis and X-ray crystal structures of N-(3-deoxy-3-acetamido-β-D-glycopyranosyl)acetamide (Glc3NAcβNHAc) and the corresponding propio...

journal_title：Glycoconjugate journal

authors： Mathiselvam M,Varghese B,Loganathan D

更新日期：2011-12-01 00:00:00

abstract：:In this study, the content, structure and residual percentages of glycosaminoglycans (GAGs) in the feces of seven breastfed newborns after ingesting a known amount of milk were studied. A comparison was made with five newborns fed with formula milk. Characterization of GAGs from milk and feces samples was performed ac...

journal_title：Glycoconjugate journal

authors： Maccari F,Mantovani V,Gabrielli O,Carlucci A,Zampini L,Galeazzi T,Galeotti F,Coppa GV,Volpi N

更新日期：2016-04-01 00:00:00

abstract：:In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45...

journal_title：Glycoconjugate journal

authors： Hernández P,Debray H,Jaekel H,Garfias Y,Jiménez Md Mdel C,Martínez-Cairo S,Zenteno E

更新日期：2001-04-01 00:00:00

abstract：:Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-alpha-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1 and RGXT2 in Pi...

journal_title：Glycoconjugate journal

authors： Petersen BL,Egelund J,Damager I,Faber K,Jensen JK,Yang Z,Bennett EP,Scheller HV,Ulvskov P

更新日期：2009-12-01 00:00:00

abstract：:As an adjunct to existing thin layer and column chromatographic methods for the identification of glycolipids a method that utilizes the high pH anion chromatographic (HPAEC) analysis of the oligosaccharides released from the glycolipids by endoglycoceramidase has been developed. Using a Dionex Carbo Pak PA1 column an...

journal_title：Glycoconjugate journal

authors： Ruan S,Lloyd KO

更新日期：1994-06-01 00:00:00

abstract：:Egg yolk, a large proportion of the egg, was studied for the preparation of N-acetylneuraminic acid (Neu5Ac). The delipidated hen egg yolk (DEY; 500 kg containing 0.2% w/w, Neu5Ac) was hydrolysed with HCl (pH 1.4) at 80 degrees C and neutralized with NaOH (pH 6.0). The mixture was filtered and electrodialysed until th...

journal_title：Glycoconjugate journal

authors： Koketsu M,Juneja LR,Kawanami H,Kim M,Yamamoto T

更新日期：1992-04-01 00:00:00

abstract：:A new strategy for the fluorometric determination of glycosyltransferase activities is reported. The method involves dansyl chloride derivatization of the reduced form (pNH2phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO2phenyl). Focusing on the Golgi enzyme core 2 N-...

journal_title：Glycoconjugate journal

authors： Palmerini CA,Datti A,Vanderelst IE,Minuti L,Orlacchio A

更新日期：1996-08-01 00:00:00