Abstract:
:Recently, we demonstrated that the human xylosyltransferase II (XT-II) has enzymatic activity and is able to catalyze the initial and rate-limiting step in the biosynthesis of glycosaminoglycans (GAGs) like chondroitin and dermatan sulfate, as well as heparan sulfate and heparin. Therefore, this enzyme also very likely assumes a crucial regulatory role in the biosynthesis of proteoglycans (PGs). In this study, we identified and characterized for the first time the XYLT2 gene promoter region and transcription factors involved in its regulation. Several binding sites for members of the Sp1 family of transcription factors were identified as being necessary for transcriptional regulation of the XYLT2 gene. This was determined by mithramycin A treatment, electrophoretic mobility shift and supershift assays, as well as numerous site-directed mutagenesis experiments. Different 5' and 3' deletion constructs of the predicted GC rich promoter region, which lacks a canonical TATA and CAAT box, revealed that a 177 nts proximal promoter element is sufficient and indispensable to drive the constitutive transcription in full strength in HepG2 hepatoma cells. In addition, we also detected the transcriptional start site using 5'-RACE (rapid amplification of cDNA ends). Our results provide an insight into transcriptional regulation of the XYLT2 gene and may contribute to understanding the manifold GAG-involving processes in health and disease.
journal_name
Glycoconj Jjournal_title
Glycoconjugate journalauthors
Müller B,Prante C,Knabbe C,Kleesiek K,Götting Cdoi
10.1007/s10719-012-9439-5subject
Has Abstractpub_date
2013-04-01 00:00:00pages
237-45issue
3eissn
0282-0080issn
1573-4986journal_volume
30pub_type
杂志文章abstract::Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis). Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be c...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-016-9759-y
更新日期:2017-04-01 00:00:00
abstract::The IgG Fc glycans strongly influence the Fcγ receptor interactions and Fc-mediated effector mechanisms. Changes in the structure of IgG glycans are associated with various diseases, such as infections and autoimmunity. However, the possible role of Fc glycans in tumor immunity is not yet fully understood. The aim of ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-011-9364-z
更新日期:2012-01-01 00:00:00
abstract::Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-008-9163-3
更新日期:2009-01-01 00:00:00
abstract::Mannose 6-phosphate receptor proteins (MPR 300 and 46) are type 1 transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. In a recent study we have purified both receptors from goat liver and raised antibodies. An ELISA method was developed that allowed quantification of both receptors in...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/B:GLYC.0000025820.56028.32
更新日期:2004-01-01 00:00:00
abstract::The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/BF01053194
更新日期:1996-12-01 00:00:00
abstract::The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy. hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB). The beta-hCG protein is encoded by CGB3, CGB5, CGB7 and CGB8 genes. CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-020-09936-w
更新日期:2020-10-01 00:00:00
abstract::Carbohydrate-carbohydrate interaction is a reliable and versatile mechanism for cell adhesion and recognition. Glycosphingolipid (GSL) clusters at the cell membrane are mainly involved in this interaction. To investigate carbohydrate-carbohydrate interaction an integrated strategy (Glyconanotechnology) was developed. ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1023/B:GLYC.0000044846.80014.cb
更新日期:2004-01-01 00:00:00
abstract::Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens o...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-019-09882-2
更新日期:2019-10-01 00:00:00
abstract::UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II (GnT II; EC 2.4.1.143) is a medial-Golgi resident enzyme that catalyses an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides. Screening a cDNA library from Xenopus laevis ovary with a human ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/A:1024201824354
更新日期:2002-03-01 00:00:00
abstract::UDP-GlcNAc:Man alpha 1-3R beta 1-2-N-acetylglucosaminyltransferase I (GlcNAc-T I; EC 2.4.1.101) catalyses the conversion of [Man alpha 1-6(Man alpha 1-3)Man alpha 1-6][Man alpha 1-3]Man beta-O-R to [Man alpha 1-6(Man alpha 1-3)Man alpha 1-6] [GlcNAc beta 1-2Man alpha 1-3]Man beta-O-R (R = 1-4GlcNAc beta 1-4GlcNAc- Asn...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/BF00731234
更新日期:1995-12-01 00:00:00
abstract::The sap-sucking homopteran insects, commonly known as aphids and leafhoppers are responsible for a huge amount of lost productivity of mustard, chickpea, cabbage, rice and many other important crops. Due to their unique feeding habits and ability to build up a huge population in a very short time, they are very diffic...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/B:GLYC.0000043288.72051.7c
更新日期:2004-01-01 00:00:00
abstract::Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/a:1006935227730
更新日期:1998-08-01 00:00:00
abstract::Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embr...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1007/s10719-016-9757-0
更新日期:2017-04-01 00:00:00
abstract::C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excret...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-017-9807-2
更新日期:2018-04-01 00:00:00
abstract::Sulfated galactans (SGs) are highly anionic marine galactose-composed homopolysaccharides. Although their structures vary among species, their main features are conserved among phyla. Green algal SGs are quite heterogeneous, although preponderantly composed of 3-beta-D-Galp units. The red algal SGs (like agar and carr...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1007/s10719-009-9251-z
更新日期:2010-01-01 00:00:00
abstract::Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20-40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but research is in progress to...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1023/a:1026436424881
更新日期:1999-02-01 00:00:00
abstract::Cultured chondrocytes derived from the caudal and cephalic ends of embryonic chick sterna have been compared with each other and with whole sternum, by using a panel of 21 lectins to probe the distribution of oligosaccharides in glycoconjugates of cells and matrix at various times of culture or development. On culture...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/a:1018526813159
更新日期:1997-04-01 00:00:00
abstract::Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-013-9471-0
更新日期:2013-10-01 00:00:00
abstract::A gene encoding a putative sialidase was identified in the genome of the opportunistic fungal pathogen, Aspergillus fumigatus. Computational analysis showed that this protein has Asp box and FRIP domains, it was predicted to have an extracellular localization, and a mass of 42 kDa, all of which are characteristics of ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-010-9299-9
更新日期:2010-07-01 00:00:00
abstract::The novel glycosphingolipid, SEGLx (Galbeta1-4(Fucalpha1-3)Glcbeta1-3GalbetaCer), which was identified by us (Kawakami Y, et al. (1993) J Biochem 114: 677-83), shows a characteristic spectrum on 1H-NMR analysis, in which the anomeric proton resonances of a reducing end galactose and a glucose are split. To elucidate t...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/a:1006908020370
更新日期:1998-02-01 00:00:00
abstract::Coarse tea is made of mature tea plant (Camellia sinensis L.) shoots and is generally discarded as a worthless crop product, but has been proved an excellent material for the treatment of diabetes. This study aims to evaluate the effects of the extraction techniques WE (water extraction), UAE (ultrasound-assisted extr...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-019-09901-2
更新日期:2020-04-01 00:00:00
abstract::Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology. Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface. The major stru...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-018-9845-4
更新日期:2018-12-01 00:00:00
abstract::Gastric carcinoma is a major cause of cancer death worldwide and, like most human cancers, probably develops after environmental insults acting on normal individuals and/or individuals with increased genetic susceptibility. Mucins are attractive molecules to study the relationship between genetics and environment beca...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/a:1018573201347
更新日期:1997-01-01 00:00:00
abstract::The hallmarks of cancer are characterized by functional capabilities that allow cancer cells to survive, proliferate and disseminate during the multistep tumorigenesis. Cancer being a cellular disease, changes in cellular glycoproteins play an important role in malignant transformation and cancer progression. The pres...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1007/s10719-016-9755-2
更新日期:2017-04-01 00:00:00
abstract::A group of fluorescent statistical glycopolymers, prepared via reversible addition-fragmentation chain-transfer (RAFT)-based polymerizations, were successfully employed in lectin-mediated bacterial binding studies. The resultant glycopolymers contained three different monomers: N-(2-hydroxyethyl) acrylamide (HEAA), N-...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-013-9508-4
更新日期:2014-02-01 00:00:00
abstract::The threat of pandemic influenza is a significant concern of governments worldwide. There is a very limited and relatively expensive armament to tackle such a pandemic should it occur. This fact provides much impetus to the scientific community for the discovery of new and less expensive anti-influenza drugs. Our long...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/s10719-006-5445-9
更新日期:2006-02-01 00:00:00
abstract::The carbohydrate determinants, sialyl Lewis A and sialyl Lewis X, which are frequently expressed on human cancer cells, serve as ligands for a cell adhesion molecule of the selectin family, E-selectin, which is expressed on vascular endothelial cells. These carbohydrate determinants are involved in the adhesion of can...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1023/a:1018532409041
更新日期:1997-08-01 00:00:00
abstract::In this study, the antibody 3G9-A was assayed for activity against human erythrocyte glycosphingolipids. The antibody was found to recognize glycosphingolipid components from blood group A erythrocytes but not glycosphingolipids from blood group B or O erythrocytes. Subsequent investigation revealed that the glycosphi...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/BF00731482
更新日期:1996-06-01 00:00:00
abstract::Galectins are animal lectins that can specifically bind beta-galactosides. Thirteen galectins have already been described. This review focuses on a specific member of this family: galectin-8. This galectin was discovered in prostate cancer cells eight years ago and has been studied extensively in the last few years. T...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1023/B:GLYC.0000014086.38343.98
更新日期:2002-01-01 00:00:00
abstract::We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction ...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1023/a:1006986104865
更新日期:1998-04-01 00:00:00