Identification and characterization of the 4-epimerase AglW from the archaeon Methanococcus maripaludis.

Abstract:

:Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology. Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface. The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella. The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide. The goal of our work was to biochemically demonstrate the 4-epimerase activity of AglW, and to develop assays to determine its substrate specificity and properties. We carried out assays using UDP-Galactose, UDP-Glucose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine and N-acetylglucosamine/N-acetylgalactosamine-diphosphate - lipid as substrates, coupled with specific glycosyltransferases. We showed that AglW has a broad specificity towards UDP-sugars and that Tyr151 within a conserved YxxxK sequon is essential for the 4-epimerase function of AglW. The glycosyltransferase-coupled assays are generally useful for the identification and specificity studies of novel 4-epimerases.

journal_name

Glycoconj J

journal_title

Glycoconjugate journal

authors

Sharma S,Ding Y,Jarrell KF,Brockhausen I

doi

10.1007/s10719-018-9845-4

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

525-535

issue

6

eissn

0282-0080

issn

1573-4986

pii

10.1007/s10719-018-9845-4

journal_volume

35

pub_type

杂志文章
  • Immunochemical characterization of synthetic hexa-, octa- and decasaccharide conjugate vaccines for Vibrio cholerae O:1 serotype Ogawa with emphasis on antigenic density and chain length.

    abstract::Cholera remains to be a global health problem without suitable vaccines for endemic control or outbreak relief. Here we describe a new parenteral vaccine based on neoglyco-conjugate of synthetic fragments of O-specific polysaccharide (O-SP) of Vibrio cholerae O1, serotype Ogawa. Hexa-, octa- and decasaccharides of the...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-013-9491-9

    authors: Ftacek P,Nelson V,Szu SC

    更新日期:2013-12-01 00:00:00

  • Preparation of N-acetylneuraminic acid from delipidated egg yolk.

    abstract::Egg yolk, a large proportion of the egg, was studied for the preparation of N-acetylneuraminic acid (Neu5Ac). The delipidated hen egg yolk (DEY; 500 kg containing 0.2% w/w, Neu5Ac) was hydrolysed with HCl (pH 1.4) at 80 degrees C and neutralized with NaOH (pH 6.0). The mixture was filtered and electrodialysed until th...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/BF00731701

    authors: Koketsu M,Juneja LR,Kawanami H,Kim M,Yamamoto T

    更新日期:1992-04-01 00:00:00

  • Isolation and characterization of the potential receptor for wheat germ agglutinin from human neutrophils.

    abstract::Neutrophils participate in host protection and central to this process is the regulation of oxidative mechanisms. We purified by affinity chromatography the receptor for the GlcNAc-specific WGA from CD14+ CD16+ cell lysates (WGAr). The receptor is a 141 kDa glycoprotein constituted by two subunits of 78 and 63 kDa. It...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-006-8635-6

    authors: Solórzano C,Bouquelet S,Pereyra MA,Blanco-Favela F,Slomianny MC,Chavez R,Lascurain R,Zenteno E,Agundis C

    更新日期:2006-11-01 00:00:00

  • Alpha 1,4galactosyltransferase activity and Gb3Cer expression in human leukaemia/lymphoma cell lines.

    abstract::We have used two methods to evaluate the level of expression of Gb3Cer in several human leukaemia/lymphoma cell lines representative of the myeloid (K562, KG-1, HL-60, and lymphoid (Reh, Daudi, Raji, RPMI 8226, CCRF-CEM, MOLT-4) lineages blocked at varied stages of differentiation. TLC immunostaining of glycolipid ext...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/BF00731265

    authors: Stults CL,Larsen RD,Macher BA

    更新日期:1995-10-01 00:00:00

  • Developmentally regulated O-acetylated sialoglycans in the central nervous system revealed by a new monoclonal antibody 493D4 recognizing a wide range of O-acetylated glycoconjugates.

    abstract::We have previously detected an alkali-labile and developmentally regulated antigen in rat embryonic cerebral cortex, which may be 9-O-acetylsialylated GT3 ganglioside (Hirabayashi Y, Hirota M, Suzuki Y, Matsumoto M, Obata K, Ando S (1989) Neurosci Lett 106:193-98). In this study we established a mouse monoclonal antib...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1018542105832

    authors: Zhang G,Ji L,Kurono S,Fujita SC,Furuya S,Hirabayashi Y

    更新日期:1997-11-01 00:00:00

  • The structure of the keratan sulphate chains attached to fibromodulin from human articular cartilage.

    abstract::The small keratan sulphate proteoglycan, fibromodulin, has been isolated from pooled human articular cartilage. The main chain repeat region and the chain caps from the attached N-linked keratan sulphate chains have been fragmented by keratanase II digestion, and the oligosaccharides generated have been reduced and is...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1018552913584

    authors: Lauder RM,Huckerby TN,Nieduszynski IA

    更新日期:1997-08-01 00:00:00

  • Sulfated sialic acid-polymers inhibit the cytotoxic action of bee and snake venom.

    abstract::Colominic acid is an alpha2,8-linked sialic acid polymer produced by Escherichia coli. We found that synthetic sulfated-colominic acids (SC) remarkably inhibited the cytotoxicity of bee and snake venom toward mouse fibroblast cells, but colominic acids showed no inhibition themselves, indicating the important role of ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1007074410201

    authors: Oda Y,Kinoshita M,Hamada K,Nakayama K,Ohta Y,Yamaguchi S,Tsukada Y,Kawai Y,Kakehi K

    更新日期:1999-08-01 00:00:00

  • Expression analysis of a type S2 EUL-related lectin from rice in Pichia pastoris.

    abstract::Rice (Oryza sativa) expresses different putative carbohydrate-binding proteins belonging to the class of lectins containing an Euonymus lectin (EUL)-related domain, one of them being OrysaEULS2. The OrysaEULS2 sequence consists of a 56 amino acid N-terminal domain followed by the EUL sequence. In this paper the origin...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-012-9405-2

    authors: Al Atalah B,Rougé P,Smith DF,Proost P,Lasanajak Y,Van Damme EJ

    更新日期:2012-10-01 00:00:00

  • Structure of the main ganglioside from the brain of Xenopus laevis.

    abstract::The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay pro...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1022584914778

    authors: Rizzo AM,Berra B,Rossi F,Guerra A,Gornati R,Bernardini G,Taki T,Kasama T,Mauri L,Sonnino S

    更新日期:2002-01-01 00:00:00

  • A novel method for determination of alpha1,6fucosyltransferase activity using a reducing oligosaccharide from egg yolk as a specific acceptor.

    abstract::A new method for determination of alpha1,6fucosyltransferase activity has been described. Recently, the disialyl-biantennary undecasaccharide was prepared in high yield from egg yolk [(1996), Carbohydr Lett 2: 137-42]. By treatment of this oligosaccharide with neuraminidase and beta-galactosidase, we readily obtained ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1006954915102

    authors: Yazawa S,Kochibe N,Nishimura T,Shima C,Takai I,Adachi M,Asao T,Hada T,Enoki Y,Juneja LR

    更新日期:1998-09-01 00:00:00

  • One-step immunopurification and lectinochemical characterization of the Duffy atypical chemokine receptor from human erythrocytes.

    abstract::Duffy antigen/receptor for chemokines (DARC) is a glycosylated seven-transmembrane protein acting as a blood group antigen, a chemokine binding protein and a receptor for Plasmodium vivax malaria parasite. It is present on erythrocytes and endothelial cells of postcapillary venules. The N-terminal extracellular domain...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-011-9367-9

    authors: Grodecka M,Bertrand O,Karolak E,Lisowski M,Waśniowska K

    更新日期:2012-04-01 00:00:00

  • Cloning and expression of a porcine UDP-GalNAc: polypeptide N-acetylgalactosaminyl transferase.

    abstract::By employing a bovine UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyl transferase (O-GalNAc transferase) cDNA as a probe, we isolated four overlapping cDNAs from a porcine lung cDNA library. Both the nucleotide sequence of the porcine cDNA and the predicted primary structure of the protein (559 amino aci...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/BF00731244

    authors: Yoshida A,Hara T,Ikenaga H,Takeuchi M

    更新日期:1995-12-01 00:00:00

  • Multiple lectin assays for detecting glyco-alteration of serum GP73 in liver diseases.

    abstract::Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. In this study, we used multiple lectin assays to analyze glycan patterns of serum GP73 and evaluated its diagnostic value for distinguishing hepatocellular carcinoma (HCC) from liver cirrhosis (LC). Firstly, ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-015-9614-6

    authors: Jiang K,Shang S,Li W,Guo K,Qin X,Zhang S,Liu Y

    更新日期:2015-12-01 00:00:00

  • Effects of extraction methods on physicochemical properties and hypoglycemic activities of polysaccharides from coarse green tea.

    abstract::Coarse tea is made of mature tea plant (Camellia sinensis L.) shoots and is generally discarded as a worthless crop product, but has been proved an excellent material for the treatment of diabetes. This study aims to evaluate the effects of the extraction techniques WE (water extraction), UAE (ultrasound-assisted extr...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-019-09901-2

    authors: Zhu J,Chen Z,Zhou H,Yu C,Han Z,Shao S,Hu X,Wei X,Wang Y

    更新日期:2020-04-01 00:00:00

  • Structure elucidation of NeuAc, NeuGc and Kdn-containing O-glycans released from Triturus alpestris oviductal mucins. Characterization of the poly LacdiNAc sequence: HSO3(4)(GalNAcbeta1-4GlcNAcbeta1-3)1-3GalNAcbeta1-4(GlcNAcbeta1-3)0-1GlcNAcbeta1-6GalNAc-

    abstract::Eggs from Amphibia are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of highly glycosylated mucin-type glycoproteins containing up to 60% of carbohydrates, which display a remarkable species-specificity. This material obtained from Triturus alpestris was submitt...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-006-6126-4

    authors: Florea D,Maes E,Guérardel Y,Page A,Zanetta JP,Cogalniceanu D,Strecker G

    更新日期:2006-07-01 00:00:00

  • Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.

    abstract::Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presen...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-019-09868-0

    authors: Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Z

    更新日期:2019-06-01 00:00:00

  • Role of sialic acids in rotavirus infection.

    abstract::Rotaviruses are the leading cause of childhood diarrhea. The entry of rotaviruses into the host cell is a complex process that includes several interactions of the outer layer proteins of the virus with different cell surface molecules. The fact that neuraminidase treatment of the cells, or preincubation of the virus ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审

    doi:10.1007/s10719-006-5435-y

    authors: Isa P,Arias CF,López S

    更新日期:2006-02-01 00:00:00

  • Interactions of putative heparin-binding domains of basic fibroblast growth factor and its receptor, FGFR-1, with heparin using synthetic peptides.

    abstract::We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1006986104865

    authors: Kinsella L,Chen HL,Smith JA,Rudland PS,Fernig DG

    更新日期:1998-04-01 00:00:00

  • Developmental diseases caused by impaired nucleotide sugar transporters.

    abstract::Nucleotide sugar transporters play critical roles in glycosylation of proteins, lipids and proteoglycans, which are essential for organogenesis, development, mammalian cellular immunity and pathogenicity of human pathogenic agents. Functional deficiencies of these transporters result in global defects of glycoconjugat...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审

    doi:10.1007/s10719-012-9375-4

    authors: Liu L,Hirschberg CB

    更新日期:2013-01-01 00:00:00

  • Synthesis of the dodecasaccharide fragment representing the O-polysaccharide of Vibrio cholerae O:1, serotype Ogawa, bearing an aglycon offering flexibility for chemical linking to proteins.

    abstract::Two azidohexasaccharide building blocks, of which the glycosyl acceptor was the 5-(methoxycarbonyl)pentyl glycoside, were coupled using the trichloroacetimidate technology. The 12 azido functions present in the dodecasaccharide thus formed were then converted to amino groups using hydrogen sulfide as a reducing reagen...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1018591132723

    authors: Ogawa Y,Kovac P

    更新日期:1997-06-01 00:00:00

  • Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.

    abstract::Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present. Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great inter...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-018-9852-5

    authors: Baerenfaenger M,Moritz M,Meyer B

    更新日期:2019-02-01 00:00:00

  • Immune-stimulating properties of polysaccharides from Phellodendri cortex (Hwangbek).

    abstract::Heteropolysaccarides were isolated from the Korean medicinal plant, Phellodendri cortex (Hwangbek), by hot water and alkali extractions. The extracted polysaccharides were fractionated into eight fractions and they are mainly composed of D-N-acetylglucosamine, D-galactose, D-mannose, and D-glucose. Among the polysacch...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1007084506071

    authors: Park JI,Shim JK,Do JW,Kim SY,Seo EK,Kwon HJ,Lee TK,Kim JK,Choi DY,Kim CH

    更新日期:1999-03-01 00:00:00

  • Stage-specific embryonic antigen-4 is a histological marker reflecting the malignant behavior of prostate cancer.

    abstract::Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens o...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-019-09882-2

    authors: Nakamura Y,Miyata Y,Matsuo T,Shida Y,Hakariya T,Ohba K,Taima T,Ito A,Suda T,Hakomori SI,Saito S,Sakai H

    更新日期:2019-10-01 00:00:00

  • Structural features of the lysosomal hydrolase mannose 6-phosphate uncovering enzyme.

    abstract::The uncovering enzyme (UCE) removes N-acetylglucosamine from lysosomal enzymes to uncover the mannose 6-phosphate (Man-6-P) determinant necessary for targeting these enzymes to lysosomes. Failure to create the Man-6-P determinant is one cause of lysosomal storage diseases. Despite its medical importance, little struct...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-005-0846-8

    authors: Wei Y,Yen TY,Cai J,Trent JO,Pierce WM,Young WW Jr

    更新日期:2005-02-01 00:00:00

  • Cloning and sequence analysis of the Maackia amurensis haemagglutinin cDNA.

    abstract::Maackia amurensis haemagglutinin (MAH) is a leguminous lectin which preferentially binds to a cluster of sialylated O-linked carbohydrate chains (Konami Y, Yamamoto K. Osawa T, Irimura T (1994) FEBS Lett 342:334-38). In the present study a 950 bp cDNA clone encoding MAH was isolated from a cDNA library constructed fro...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/BF00731308

    authors: Yamamoto K,Ishida C,Saito M,Konami Y,Osawa T,Irimura T

    更新日期:1994-12-01 00:00:00

  • Chemical characterization of the milk oligosaccharides of some Artiodactyla species including giraffe (Giraffa camelopardalis), sitatunga (Tragelaphus spekii), deer (Cervus nippon yesoensis) and water buffalo (Bubalus bubalis).

    abstract::Mammalian milk/colostrum usually contains oligosaccharides along with the predominant disaccharide lactose. It has been found that the number and identity of these milk oligosaccharides varies among mammalian species. Oligosaccharides predominate over lactose in the milk/colostrum of Arctoidea species (Carnivora), whe...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-018-9849-0

    authors: Mineguchi Y,Miyoshi M,Taufik E,Kawamura A,Asakawa T,Suzuki I,Souma K,Okubo M,Saito T,Fukuda K,Asakuma S,Urashima T

    更新日期:2018-12-01 00:00:00

  • Enrichment and characterization of a bacterial mixture capable of utilizing C-mannosyl tryptophan as a carbon source.

    abstract::C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excret...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/s10719-017-9807-2

    authors: Hossain TJ,Manabe S,Ito Y,Iida T,Kosono S,Ueda K,Hosomi A,Inoue D,Suzuki T

    更新日期:2018-04-01 00:00:00

  • MUC1 gene polymorphism and gastric cancer--an epidemiological study.

    abstract::Gastric carcinoma is a major cause of cancer death worldwide and, like most human cancers, probably develops after environmental insults acting on normal individuals and/or individuals with increased genetic susceptibility. Mucins are attractive molecules to study the relationship between genetics and environment beca...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1018573201347

    authors: Carvalho F,Seruca R,David L,Amorim A,Seixas M,Bennett E,Clausen H,Sobrinho-Simões M

    更新日期:1997-01-01 00:00:00

  • A full assignment of proton resonances for an alpha(1-3)-linked fucose residue in keratan sulphate from bovine articular cartilage.

    abstract::Full proton NMR assignments have been achieved for the alpha(1-3)-linked fucose residues contained in alkaline borohydride reduced keratan sulphate chains derived from bovine articular cartilage. This involved 500 MHz spectroscopy at 60 degrees C and included COSY and RELAYED-COSY determinations. ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1007/BF00731641

    authors: Huckerby TN,Nieduszynski IA,Brown GM,Cockin GH

    更新日期:1991-02-01 00:00:00

  • Synthesis of Gal-beta-(1-->4)-GlcNac-beta-(1-->6)-[Gal-beta-(1-->3]-GalNAc-alpha- OBn oligosaccharides bearing O-methyl or O-sulfo groups at C-3 of the Gal residue: specific acceptors for Gal: 3-O-sulfotransferases.

    abstract::Our recent studies have revealed the existence of two distinct Gal: 3-O-sulfotransferases capable of acting on the C-3 position of galactose in a Core 2 branched structure, e.g., Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNacalph a1-->OBenzyl as acceptor to give 3-O-sulfoGalbeta1-->4GlcNAcbeta1-->3(Galbeta1-->3)G alN...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章

    doi:10.1023/a:1006977607394

    authors: Jain RK,Piskorz CF,Chandrasekaran EV,Matta KL

    更新日期:1998-10-01 00:00:00