Identification of receptors responsible for binding of the mannose specific lectin to the gut epithelial membrane of the target insects.


:The sap-sucking homopteran insects, commonly known as aphids and leafhoppers are responsible for a huge amount of lost productivity of mustard, chickpea, cabbage, rice and many other important crops. Due to their unique feeding habits and ability to build up a huge population in a very short time, they are very difficult to control. The objective of the ongoing program is to develop insect-resistant crop species through genetic engineering techniques to combat the yield losses, which necessitates the identification of appropriate control elements. In this direction, mannose-binding 25 kDa lectins have been purified from leaves of garlic, Diffenbachia sequina and tubers of Colocasia esculanta. The purified lectins have been analyzed in SDS-PAGE. The effectiveness of these lectins against chickpea aphids, mustard aphids and green leaf hoppers of rice have been tested. The LC(50) value of each lectin against different insects had been monitored [1,2]. Through immunolocalization analysis, the binding of the lectin had been demonstrated at the epithelial membrane of the midgut of the lectin-treated insects [1]. Receptor proteins of brush border membrane vesicle (BBMV) of the target insects, responsible for binding of the lectin to the midgut of the epithelial layer have been purified and analyzed through ligand assay. Biochemical studies have been undertaken to investigate the lectin-receptor interaction at molecular level.


Glycoconj J


Glycoconjugate journal


Majumder P,Banerjee S,Das S




Has Abstract


2004-01-01 00:00:00














  • Modulation of the basal activity of phosphatidylinositol-3-kinase/protein kinase B signaling pathway in human hepatocarcinoma cells.

    abstract::The modulation of GnT-V activity by signaling molecules in PI-3-K/PKB pathway in human hepatocarcinoma cell line 7721 was studied. GnT-V activity was determined after the transfection of sense or antisense cDNA of PKB into the cells, as well as the addition of activators, specific inhibitors, and the antibodies to the...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Guo HB,Shen ZH,Huang CX,Ma J,Huang Y,Chen HL

    更新日期:2000-05-01 00:00:00

  • A novel method for determination of alpha1,6fucosyltransferase activity using a reducing oligosaccharide from egg yolk as a specific acceptor.

    abstract::A new method for determination of alpha1,6fucosyltransferase activity has been described. Recently, the disialyl-biantennary undecasaccharide was prepared in high yield from egg yolk [(1996), Carbohydr Lett 2: 137-42]. By treatment of this oligosaccharide with neuraminidase and beta-galactosidase, we readily obtained ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Yazawa S,Kochibe N,Nishimura T,Shima C,Takai I,Adachi M,Asao T,Hada T,Enoki Y,Juneja LR

    更新日期:1998-09-01 00:00:00

  • Two closely related forms of UDP-GlcNAc: alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II occur in the clawed frog Xenopus laevis.

    abstract::UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II (GnT II; EC is a medial-Golgi resident enzyme that catalyses an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides. Screening a cDNA library from Xenopus laevis ovary with a human ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Mucha J,Svoboda B,Kappel S,Strasser R,Bencur P,Fröhwein U,Schachter H,Mach L,Glössl J

    更新日期:2002-03-01 00:00:00

  • Understanding carbohydrate-carbohydrate interactions by means of glyconanotechnology.

    abstract::Carbohydrate-carbohydrate interaction is a reliable and versatile mechanism for cell adhesion and recognition. Glycosphingolipid (GSL) clusters at the cell membrane are mainly involved in this interaction. To investigate carbohydrate-carbohydrate interaction an integrated strategy (Glyconanotechnology) was developed. ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: de la Fuente JM,Penadés S

    更新日期:2004-01-01 00:00:00

  • Chemical characterization of the lectin from Amaranthus leucocarpus syn. hypocondriacus by 2-D proteome analysis.

    abstract::In this work, we characterized chemically the N-acetyl-D-galactosamine specific lectin from Amaranthus leucocarpus syn hypocondriacus lectin (ALL). It is a dimeric glycoprotein composed by three isoforms with pl at 4.8, 4.9, and 5.2. Circular dichroism analysis indicated that the secondary structure of ALL contains 45...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Hernández P,Debray H,Jaekel H,Garfias Y,Jiménez Md Mdel C,Martínez-Cairo S,Zenteno E

    更新日期:2001-04-01 00:00:00

  • Glycan microarrays of fluorescently-tagged natural glycans.

    abstract::This review discusses the challenges facing research in 'functional glycomics' and the novel technologies that are being developed to advance the field. The structural complexity of glycans and glycoconjugates makes studies of both their structures and recognition difficult. However, these intricate structures can be ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Song X,Heimburg-Molinaro J,Smith DF,Cummings RD

    更新日期:2015-10-01 00:00:00

  • Site-specific characterization of N-linked glycosylation in human urinary glycoproteins and endogenous glycopeptides.

    abstract::Glycosylation is a very important post-translational modification involved in various cellular processes, such as cell adhesion, signal transduction and immune response. Urine is a rich source of glycoproteins and attractive biological fluid for biomarker discovery, owing to its availability, ease of collection, and c...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Kawahara R,Saad J,Angeli CB,Palmisano G

    更新日期:2016-12-01 00:00:00

  • Biomolecular analysis of matrix proteoglycans as biomarkers in non small cell lung cancer.

    abstract::Matrix proteoglycans (PGs) have shown promise as biomarker in malignancies. We employed agarose gel eletrophoresis, quantitative real- time reverse transcription-polymerase chain reaction and immunohistochemistry to evaluate the content of sulfated glicosaminoglycans (chondroitin sulfate and heparan sulfate) and expre...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Rangel MP,de Sá VK,Prieto T,Martins JRM,Olivieri ER,Carraro D,Takagaki T,Capelozzi VL

    更新日期:2018-04-01 00:00:00

  • Interleukin-3-associated ganglioside GD1a is induced independently of normal interleukin-3 receptor in murine myelogenous leukaemia NFS60 cells transfected with the interleukin-3 gene.

    abstract::The mechanism of interleukin-3 (IL-3) independent cell growth and of IL-3-associated ganglioside expression was analysed using the IL-3 dependent murine myelogenous leukaemia cell line NFS60-I7 and IL-3 gene-transfected sublines. The transfected cell lines showed autonomous cell growth, tumorigenicity, and IL-3 associ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Nakamura M,Kirito K,Tsunoda A,Hara K,Furukawa Y,Saito M

    更新日期:1996-04-01 00:00:00

  • Three-dimensional structure of a glycosphingolipid having a novel carbohydrate linkage, Galbeta1-4(Fucalpha1-3)Glcbeta1-3Galbeta, determined by theoretical calculations.

    abstract::The novel glycosphingolipid, SEGLx (Galbeta1-4(Fucalpha1-3)Glcbeta1-3GalbetaCer), which was identified by us (Kawakami Y, et al. (1993) J Biochem 114: 677-83), shows a characteristic spectrum on 1H-NMR analysis, in which the anomeric proton resonances of a reducing end galactose and a glucose are split. To elucidate t...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Kawakami J,Kawakami Y,Nakamura K,Kojima H,Ito S,Tamai Y

    更新日期:1998-02-01 00:00:00

  • Epigenetic regulation of ganglioside expression in neural stem cells and neuronal cells.

    abstract::The structural diversity and localization of cell surface glycosphingolipids (GSLs), including gangliosides, in glycolipid-enriched microdomains (GEMs, also known as lipid rafts) render them ideally suited to play important roles in mediating intercellular recognition, interactions, adhesion, receptor function, and si...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Itokazu Y,Tsai YT,Yu RK

    更新日期:2017-12-01 00:00:00

  • Structural characterization of blood group A glycosphingolipids recognized by the antibody 3G9-A.

    abstract::In this study, the antibody 3G9-A was assayed for activity against human erythrocyte glycosphingolipids. The antibody was found to recognize glycosphingolipid components from blood group A erythrocytes but not glycosphingolipids from blood group B or O erythrocytes. Subsequent investigation revealed that the glycosphi...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Briggs JB,Mandrell R,Macher BA

    更新日期:1996-06-01 00:00:00

  • Molecular cloning of goat Mannose 6-phosphate receptors, MPR 300 and 46.

    abstract::Mannose 6-phosphate receptor proteins (MPR 300 and 46) are type 1 transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. In a recent study we have purified both receptors from goat liver and raised antibodies. An ELISA method was developed that allowed quantification of both receptors in...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Suresh K,Vegiraju SR,Nadimpalli SK

    更新日期:2004-01-01 00:00:00

  • Role of sialic acids in rotavirus infection.

    abstract::Rotaviruses are the leading cause of childhood diarrhea. The entry of rotaviruses into the host cell is a complex process that includes several interactions of the outer layer proteins of the virus with different cell surface molecules. The fact that neuraminidase treatment of the cells, or preincubation of the virus ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Isa P,Arias CF,López S

    更新日期:2006-02-01 00:00:00

  • Salivary glyco-sialylation changes monitors oral carcinogenesis.

    abstract::Alterations in cell membrane glycosylation play important role in oral carcinogenesis. The present study evaluated salivary sialylation changes i.e. total sialic acid (TSA), sialidase activity, linkage specific (α2-3 and α2-6) sialoproteins and sialyl transferase (ST) activity in controls, patients with oral precancer...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Vajaria BN,Patel KR,Begum R,Patel JB,Shah FD,Joshi GM,Patel PS

    更新日期:2014-12-01 00:00:00

  • Glycation changes the charge distribution of type I collagen fibrils.

    abstract::In aging and diabetes, glycation of collagen molecules leads to the formation of cross-links that could alter the surface charge on collagen fibrils, and hence affect the properties and correct functioning of a number of tissues. The electron-optical stain phosphotungstic acid (PTA) binds to positively charged amino a...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Hadley JC,Meek KM,Malik NS

    更新日期:1998-08-01 00:00:00

  • Glycotargeting to improve cellular delivery efficiency of nucleic acids.

    abstract::Nucleic acids bearing glycans of various structures have been under vigorous investigation in the past decade. The carbohydrate moieties of such complexes can serve as recognition sites for carbohydrate-binding proteins-lectins-and initiate receptor-mediated endocytosis. Therefore, carbohydrates can enhance cell targe...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Yan H,Tram K

    更新日期:2007-04-01 00:00:00

  • Expression of the T antigen on a T-lymphoid cell line, supT1.

    abstract::We have measured glycosyltransferase activities of SupT1 cells, a T-lymphoid cell line shown to react with autoantibodies in the sera of many HIV patients. Since considerable alpha-N-acetylgalactosaminyl-transferase and beta 1, 3 galactosyltransferase activities were found in SupT1 cells, at least the O-glycan core 1 ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Nakada H,Inoue M,Tanaka N,Wakamiya N,Yamashina I

    更新日期:1995-06-01 00:00:00

  • The preparation of a cold-water soluble polysaccharide from Grifola frondosa and its inhibitory effects on MKN-45 cells.

    abstract::In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatogr...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Liu C,Ji HY,Wu P,Yu J,Liu AJ

    更新日期:2020-08-01 00:00:00

  • Carbohydrate analysis throughout the development of a protein therapeutic.

    abstract::This review discusses the challenges involved in the characterization of the glycosylation of therapeutic glycoproteins. The focus is on methods that are most commonly used in regulatory filings and lot release testing of therapeutic glycoproteins. The different types of assays for carbohydrate analysis are reviewed, ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Higgins E

    更新日期:2010-02-01 00:00:00

  • Plasmatic kinetics of dermatan sulfate during enzyme replacement therapy with iduronate-2-sulfatase in a mucopolysaccharidosis II patient.

    abstract::Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Volpi N,Zampini L,Maccari F,Santoro L,Galeotti F,Galeazzi T,Gabrielli O,Coppa GV

    更新日期:2013-10-01 00:00:00

  • Glycation of human lens proteins from diabetic and (nondiabetic) senile cataract patients.

    abstract::Glycation (nonenzymatic glycosylation) in the human lens (cortex and nucleus) in senile (nondiabetic) and diabetic cataracts was studied by measuring the extent of early and late glycation products, the content of free epsilon-amino groups and the formation of disulfide bonds in the soluble lens proteins. There was a ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Duhaiman AS

    更新日期:1995-10-01 00:00:00

  • Action of rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase on Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe and GlcNAc beta 1-4GlcNAc beta-OMe as synthetic substrates.

    abstract::Incubation of synthetic Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe, and GlcNAc beta 1-4GlcNAc beta-OMe with CMP-Neu5Ac and rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase resulted in the formation of Neu5Ac alpha 2-6Man beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6Ga...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Hokke CH,van der Ven JG,Kamerling JP,Vliegenthart JF

    更新日期:1993-02-01 00:00:00

  • Alpha 1,4galactosyltransferase activity and Gb3Cer expression in human leukaemia/lymphoma cell lines.

    abstract::We have used two methods to evaluate the level of expression of Gb3Cer in several human leukaemia/lymphoma cell lines representative of the myeloid (K562, KG-1, HL-60, and lymphoid (Reh, Daudi, Raji, RPMI 8226, CCRF-CEM, MOLT-4) lineages blocked at varied stages of differentiation. TLC immunostaining of glycolipid ext...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Stults CL,Larsen RD,Macher BA

    更新日期:1995-10-01 00:00:00

  • Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid.

    abstract::Based on the O-specific polysaccharides of the lipopolysaccharides (O-polysaccharides, O-antigens), strains of a clonal species Escherichia coli are classified into 184 O serogroups. In this work, structures of the O-polysaccharides of E. coli O69 and O146 were elucidated and gene clusters for their biosynthesis were ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Knirel YA,Guo X,Senchenkova SN,Perepelov AV,Liu B,Shashkov AS

    更新日期:2017-02-01 00:00:00

  • A case for protein-level and site-level specificity in glycoproteomic studies of disease.

    abstract::Abnormal glycosylation of proteins is known to be either resultant or causative of a variety of diseases. This makes glycoproteins appealing targets as potential biomarkers and focal points of molecular studies on the development and progression of human ailment. To date, a majority of efforts in disease glycoproteomi...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Schumacher KN,Dodds ED

    更新日期:2016-06-01 00:00:00

  • Concise synthesis of a heptasaccharide antigen found in the cell-wall lipopolysaccharide of Mycobacterium gordonae strain 990.

    abstract::A straight forward synthesis of a heptasaccharide part of the cell-wall lipopolysaccharide of Mycobacterium gordonae strain 990, known to have antigenicity, has been achieved in excellent yield. Judicious choice of protecting groups in the intermediates played a significant role throughout the synthesis. Most of the i...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Panchadhayee R,Kumar Misra A

    更新日期:2008-12-01 00:00:00

  • Quantitation of Glycopeptides by ESI/MS - size of the peptide part strongly affects the relative proportions and allows discovery of new glycan compositions of Ceruloplasmin.

    abstract::Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present. Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great inter...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Baerenfaenger M,Moritz M,Meyer B

    更新日期:2019-02-01 00:00:00

  • Thermodynamic parameters of the interaction of Urtica dioica agglutinin with N-acetylglucosamine and its oligomers.

    abstract::The interaction between Urtica dioica agglutinin (UDA) and N-acetylglucosamine (GlcNAc) and its beta(1-4)-linked oligomers was studied by fluorescence titration and isothermal titration microcalorimetry. UDA possesses one significant binding site that can be measured calorimetrically. This site is composed of three su...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Lee RT,Gabius HJ,Lee YC

    更新日期:1998-07-01 00:00:00

  • Abnormal biantennary sugar chains are expressed in human chorionic gonadotropin produced in the choriocarcinoma cell line, JEG-3.

    abstract::Over the past two decades, sugar chain structures of human chorionic gonadotropin (hCG) produced in healthy people, three types of trophoblastic disease, and some types of cell lines have been analyzed. The abnormal biantennary structure of hCG is a good marker for the diagnosis of malignant choriocarcinoma. In spite ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Takamatsu S,Katsumata T,Inoue N,Watanabe T,Fujibayashi Y,Takeuchi M

    更新日期:2004-01-01 00:00:00