Structure elucidation of NeuAc, NeuGc and Kdn-containing O-glycans released from Triturus alpestris oviductal mucins. Characterization of the poly LacdiNAc sequence: HSO3(4)(GalNAcbeta1-4GlcNAcbeta1-3)1-3GalNAcbeta1-4(GlcNAcbeta1-3)0-1GlcNAcbeta1-6GalNAc-

abstract：:Carbohydrate-carbohydrate interaction is a reliable and versatile mechanism for cell adhesion and recognition. Glycosphingolipid (GSL) clusters at the cell membrane are mainly involved in this interaction. To investigate carbohydrate-carbohydrate interaction an integrated strategy (Glyconanotechnology) was developed. ...

journal_title：Glycoconjugate journal

authors： de la Fuente JM,Penadés S

更新日期：2004-01-01 00:00:00

abstract：:Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis). Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be c...

journal_title：Glycoconjugate journal

authors： Lin N,Mo X,Yang Y,Zhang H

更新日期：2017-04-01 00:00:00

abstract：:GM3 ganglioside interacts specifically with complex-type N-linked glycans having multivalent GlcNAc termini, as shown for (1) and (2) below. (1) Oligosaccharides (OS) isolated from ConA-non-binding N-linked glycans of ovalbumin, whose structures were identified as penta-antennary complex-type with bisecting GlcNAc, ha...

journal_title：Glycoconjugate journal

authors： Yoon SJ,Nakayama K,Takahashi N,Yagi H,Utkina N,Wang HY,Kato K,Sadilek M,Hakomori SI

更新日期：2006-12-01 00:00:00

abstract：:In this study we report on the first mass spectrometric (MS) investigation of gangliosides and preliminary assessment of the expression and structure in normal fetal neocortex in early developmental stages: 14th (Neo14) and 16th (Neo16) gestational weeks. Ganglioside analysis was carried out using a hybrid quadrupole ...

journal_title：Glycoconjugate journal

authors： Ghiulai RM,Sarbu M,Vukelić Ž,Ilie C,Zamfir AD

更新日期：2014-04-01 00:00:00

abstract：:By employing a bovine UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyl transferase (O-GalNAc transferase) cDNA as a probe, we isolated four overlapping cDNAs from a porcine lung cDNA library. Both the nucleotide sequence of the porcine cDNA and the predicted primary structure of the protein (559 amino aci...

journal_title：Glycoconjugate journal

authors： Yoshida A,Hara T,Ikenaga H,Takeuchi M

更新日期：1995-12-01 00:00:00

abstract：:Two azidohexasaccharide building blocks, of which the glycosyl acceptor was the 5-(methoxycarbonyl)pentyl glycoside, were coupled using the trichloroacetimidate technology. The 12 azido functions present in the dodecasaccharide thus formed were then converted to amino groups using hydrogen sulfide as a reducing reagen...

journal_title：Glycoconjugate journal

authors： Ogawa Y,Kovac P

更新日期：1997-06-01 00:00:00

abstract：:The N-glycan moiety of ascorbic acid oxidase from zucchini (Cucurbita pepo) has been described to be a core-pentasaccharide with a xylose [D'Andrea et al. (1988) Glycoconjugate J 5:151-7]. Ascorbic acid oxidase is sometimes used to characterize antibodies directed against carbohydrate determinants on plant glycoprotei...

journal_title：Glycoconjugate journal

authors： Altmann F

更新日期：1998-01-01 00:00:00

abstract：:Jacalin and M. pomifera agglutinin are T-antigen specific lectins with alpha 4 beta 4 structures that show far greater micro-heterogeneity than plant lectins from other families, due to multiple genetic isoforms and post-translational processing. Electrospray mass spectrometry and combined liquid chromatography-electr...

journal_title：Glycoconjugate journal

authors： Young NM,Watson DC,Thibault P

更新日期：1995-04-01 00:00:00

abstract：:The current study aims to determine the healing activity of water soluble polysaccharide-rich fraction of a wild mushroom, Termitomyces eurhizus (TEps) against the indomethacin induced gastric ulceration in mice model. Gastric tissue histology, myeloperoxidase (MPO) activity, cyclooxygenases (COX) 1 and 2 expression, ...

journal_title：Glycoconjugate journal

authors： Chatterjee A,Khatua S,Chatterjee S,Mukherjee S,Mukherjee A,Paloi S,Acharya K,Bandyopadhyay SK

更新日期：2013-11-01 00:00:00

abstract：:Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, of...

journal_title：Glycoconjugate journal

authors： Lima MA,Cavalheiro RP,M Viana G,Meneghetti MCZ,Rudd TR,Skidmore MA,Powell AK,Yates EA

更新日期：2017-06-01 00:00:00

abstract：:If we predict the host range of new or mutant influenza virus in advance, we are able to measure against pandemic human influenza immediately after the new virus emerges somewhere. Influenza viral hemagglutinin(HA)-sialoside receptor interaction is a target event for in silico chemical prediction studies about the vir...

journal_title：Glycoconjugate journal

authors： Sawada T,Hashimoto T,Tokiwa H,Suzuki T,Nakano H,Ishida H,Kiso M,Suzuki Y

更新日期：2008-12-01 00:00:00

abstract：:Human Gb3/CD77 synthase (α1,4-galactosyltransferase) is the only known glycosyltransferase that changes acceptor specificity because of a point mutation. The enzyme, encoded by A4GALT locus, is responsible for biosynthesis of Gal(α1-4)Gal moiety in Gb3 (CD77, Pk antigen) and P1 glycosphingolipids. We showed before tha...

journal_title：Glycoconjugate journal

authors： Kaczmarek R,Mikolajewicz K,Szymczak K,Duk M,Majorczyk E,Krop-Watorek A,Buczkowska A,Czerwinski M

更新日期：2016-12-01 00:00:00

abstract：:Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A numbe...

journal_title：Glycoconjugate journal

authors： Nemčovičová I,Šesták S,Rendić D,Plšková M,Mucha J,Wilson IB

更新日期：2013-12-01 00:00:00

abstract：:Fractions were prepared from the water-soluble components of Aspergillus fumigatus mycelium either by lectin-affinity chromatography or salt precipitation. While they varied considerably in their amino-acid composition, each contained a preponderance of aspartic and glutamic acids. 13C-NMR spectroscopy of these fracti...

journal_title：Glycoconjugate journal

authors： Hearn VM,Griffiths BL,Gorin PA

更新日期：1989-01-01 00:00:00

abstract：:Over the last few years enormous interest has been shown in the structures of the glycan moieties of various parasite surface glycoconjugates. Structures have been determined for the glyco-components of glycosylphosphatidylinositol (GPI) protein membrane anchors, for asparagine-linked oligosaccharides, and for the gly...

journal_title：Glycoconjugate journal

authors： Zamze S

更新日期：1991-12-01 00:00:00

abstract：:N-acetylglucosaminyltransferase (GnT)-IV a is a key enzyme that catalyzes the formation of the GlcNAC β1-4 branch on the core structure of complex N-Glycans, which is the common substrate for other N-acetylglucosaminyltransferases, such as GnT-III and GnT-V. Our recent study indicates that the expression of GnT-IVa in...

journal_title：Glycoconjugate journal

authors： Fan J,Wang S,Yu S,He J,Zheng W,Zhang J

更新日期：2012-08-01 00:00:00

abstract：:The rare N-unsubstituted glucosamine (GlcNH (3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. However, it is difficult to prepare naturally-occuring, GlcNH(3)(+)-containing oligosaccharides from HS because of their low abundance, as well as the inherent problems in both e...

journal_title：Glycoconjugate journal

authors： Wei Z,Deakin JA,Blaum BS,Uhrín D,Gallagher JT,Lyon M

更新日期：2011-12-01 00:00:00

abstract：:Incubation of synthetic Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe, and GlcNAc beta 1-4GlcNAc beta-OMe with CMP-Neu5Ac and rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase resulted in the formation of Neu5Ac alpha 2-6Man beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6Ga...

journal_title：Glycoconjugate journal

authors： Hokke CH,van der Ven JG,Kamerling JP,Vliegenthart JF

更新日期：1993-02-01 00:00:00

abstract：:Proteoglycans contain a unique carbohydrate component, glycosaminoglycan, which consists of repeating, typically sulfated disaccharides, and is capable of interacting with diverse molecules. Specific, clustered arrangements of sulfate on the glycosaminoglycan backbone form binding sites for many biologically important...

journal_title：Glycoconjugate journal

authors： Yanagishita M,Podyma-Inoue KA,Yokoyama M

更新日期：2009-11-01 00:00:00

abstract：:To elucidate the mechanism underlying the hydrolysis of the GalNAcbeta1-->4Gal linkage in ganglioside GM2 [GalNAcbeta1-->4(NeuAcalpha2-->3)Galbeta1-->4Glcbeta1-->1' Cer] by beta-hexosaminidase A (Hex A) with GM2 activator protein, we designed and synthesized two kinds of GM2 linkage analogues-6'-NeuAc-GM2 and alpha-Ga...

journal_title：Glycoconjugate journal

authors： Komori T,Ando T,Imamura A,Li YT,Ishida H,Kiso M

更新日期：2008-10-01 00:00:00

abstract：:As an adjunct to existing thin layer and column chromatographic methods for the identification of glycolipids a method that utilizes the high pH anion chromatographic (HPAEC) analysis of the oligosaccharides released from the glycolipids by endoglycoceramidase has been developed. Using a Dionex Carbo Pak PA1 column an...

journal_title：Glycoconjugate journal

authors： Ruan S,Lloyd KO

更新日期：1994-06-01 00:00:00

abstract：:Two rhamnobiose-lipid preparations have been studied by fast atom bombardment (FAB) tandem mass spectrometry. The principal rhamnobiose-lipids contain the beta-hydroxydecanoyl-beta-hydroxydecanoate Rha-Rha-C10-C10 and the beta-hydroxytetradecanoyl-beta-hydroxytetradecanoate Rha-Rha-C14-C14. Both preparations contain m...

journal_title：Glycoconjugate journal

authors： Manso Pajarron A,De Koster CG,Heerma W,Schmidt M,Haverkamp J

更新日期：1993-06-01 00:00:00

abstract：:As part of our ongoing program aimed at understanding the structural significance of GlcNAcβAsn linkage conserved in all eukaryotic N-glycoproteins, the present study reports on the synthesis and X-ray crystal structures of N-(3-deoxy-3-acetamido-β-D-glycopyranosyl)acetamide (Glc3NAcβNHAc) and the corresponding propio...

journal_title：Glycoconjugate journal

authors： Mathiselvam M,Varghese B,Loganathan D

更新日期：2011-12-01 00:00:00

abstract：:Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presen...

journal_title：Glycoconjugate journal

authors： Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Z

更新日期：2019-06-01 00:00:00

abstract：:This review summarizes fifty years of carbohydrate chemistry, from studies of reaction mechanisms and development of synthetic methods to the design of specific compounds for investigations of cancer glycoproteins. Through these years of intensive research, many carbohydrate derivatives were shown to be biologically r...

journal_title：Glycoconjugate journal

authors： Paulsen H,Brockhausen I

更新日期：2001-11-01 00:00:00

abstract：:The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...

journal_title：Glycoconjugate journal

authors： Henry S,Mollicone R,Fernandez P,Samuelsson B,Oriol R,Larson G

更新日期：1996-12-01 00:00:00

abstract：:Lubrication of mammalian joints is mediated by lubricin, a product of megakaryocyte stimulating factor gene (MSF; GenBank accession #U70136) expression. Lubricin (M(r) approximately 240 kDa) is a mucinous glycoprotein which is 50% (w/w) post-translationally modified with beta(1-3)Gal-GalNAc incompletely capped with Ne...

journal_title：Glycoconjugate journal

authors： Jay GD,Harris DA,Cha CJ

更新日期：2001-10-01 00:00:00

abstract：:We have used two methods to evaluate the level of expression of Gb3Cer in several human leukaemia/lymphoma cell lines representative of the myeloid (K562, KG-1, HL-60, and lymphoid (Reh, Daudi, Raji, RPMI 8226, CCRF-CEM, MOLT-4) lineages blocked at varied stages of differentiation. TLC immunostaining of glycolipid ext...

journal_title：Glycoconjugate journal

authors： Stults CL,Larsen RD,Macher BA

更新日期：1995-10-01 00:00:00

abstract：:Neutrophils participate in host protection and central to this process is the regulation of oxidative mechanisms. We purified by affinity chromatography the receptor for the GlcNAc-specific WGA from CD14+ CD16+ cell lysates (WGAr). The receptor is a 141 kDa glycoprotein constituted by two subunits of 78 and 63 kDa. It...

journal_title：Glycoconjugate journal

authors： Solórzano C,Bouquelet S,Pereyra MA,Blanco-Favela F,Slomianny MC,Chavez R,Lascurain R,Zenteno E,Agundis C

更新日期：2006-11-01 00:00:00

abstract：:The IgG Fc glycans strongly influence the Fcγ receptor interactions and Fc-mediated effector mechanisms. Changes in the structure of IgG glycans are associated with various diseases, such as infections and autoimmunity. However, the possible role of Fc glycans in tumor immunity is not yet fully understood. The aim of ...

journal_title：Glycoconjugate journal

authors： Kodar K,Stadlmann J,Klaamas K,Sergeyev B,Kurtenkov O

更新日期：2012-01-01 00:00:00