Synthesis of the dodecasaccharide fragment representing the O-polysaccharide of Vibrio cholerae O:1, serotype Ogawa, bearing an aglycon offering flexibility for chemical linking to proteins.

abstract：:Nucleotide sugar transporters play critical roles in glycosylation of proteins, lipids and proteoglycans, which are essential for organogenesis, development, mammalian cellular immunity and pathogenicity of human pathogenic agents. Functional deficiencies of these transporters result in global defects of glycoconjugat...

journal_title：Glycoconjugate journal

authors： Liu L,Hirschberg CB

更新日期：2013-01-01 00:00:00

abstract：:In this study, the content, structure and residual percentages of glycosaminoglycans (GAGs) in the feces of seven breastfed newborns after ingesting a known amount of milk were studied. A comparison was made with five newborns fed with formula milk. Characterization of GAGs from milk and feces samples was performed ac...

journal_title：Glycoconjugate journal

authors： Maccari F,Mantovani V,Gabrielli O,Carlucci A,Zampini L,Galeazzi T,Galeotti F,Coppa GV,Volpi N

更新日期：2016-04-01 00:00:00

abstract：:The interaction between Urtica dioica agglutinin (UDA) and N-acetylglucosamine (GlcNAc) and its beta(1-4)-linked oligomers was studied by fluorescence titration and isothermal titration microcalorimetry. UDA possesses one significant binding site that can be measured calorimetrically. This site is composed of three su...

journal_title：Glycoconjugate journal

authors： Lee RT,Gabius HJ,Lee YC

更新日期：1998-07-01 00:00:00

abstract：:The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...

journal_title：Glycoconjugate journal

authors： Henry S,Mollicone R,Fernandez P,Samuelsson B,Oriol R,Larson G

更新日期：1996-12-01 00:00:00

abstract：:Trichomonas vaginalis causes the most common non-viral sexually transmitted infection linked to increased risk of premature birth, cervical cancer and HIV. This study defines molecular domains of the parasite surface glycoconjugate lipophosphoglycan (LPG) with distinct functions in the host immunoinflammatory response...

journal_title：Glycoconjugate journal

authors： Singh BN,Hayes GR,Lucas JJ,Sommer U,Viseux N,Mirgorodskaya E,Trifonova RT,Sassi RR,Costello CE,Fichorova RN

更新日期：2009-01-01 00:00:00

abstract：:This review summarizes fifty years of carbohydrate chemistry, from studies of reaction mechanisms and development of synthetic methods to the design of specific compounds for investigations of cancer glycoproteins. Through these years of intensive research, many carbohydrate derivatives were shown to be biologically r...

journal_title：Glycoconjugate journal

authors： Paulsen H,Brockhausen I

更新日期：2001-11-01 00:00:00

abstract：:We have measured glycosyltransferase activities of SupT1 cells, a T-lymphoid cell line shown to react with autoantibodies in the sera of many HIV patients. Since considerable alpha-N-acetylgalactosaminyl-transferase and beta 1, 3 galactosyltransferase activities were found in SupT1 cells, at least the O-glycan core 1 ...

journal_title：Glycoconjugate journal

authors： Nakada H,Inoue M,Tanaka N,Wakamiya N,Yamashina I

更新日期：1995-06-01 00:00:00

abstract：:Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-alpha-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1 and RGXT2 in Pi...

journal_title：Glycoconjugate journal

authors： Petersen BL,Egelund J,Damager I,Faber K,Jensen JK,Yang Z,Bennett EP,Scheller HV,Ulvskov P

更新日期：2009-12-01 00:00:00

abstract：:To reveal the function of the carbohydrate portion of glycopeptides and glycoproteins, we chemo-enzymatically synthesized artificially N-glycosylated derivatives of eel calcitonin and studied their three-dimensional structure and biological activity. The CD and NMR spectra in trifluoroethanol-H(2)O solution showed tha...

journal_title：Glycoconjugate journal

authors： Tagashira M,Tanaka A,Hisatani K,Isogai Y,Hori M,Takamatsu S,Fujibayashi Y,Yamamoto K,Haneda K,Inazu T,Toma K

更新日期：2001-06-01 00:00:00

abstract：:We analyzed surface glycoproteins of human natural killer (NK) cells by utilizing lectins. Among the lectins tested, wheat germ agglutinin (WGA) was found to bind preferentially to CD16(Leu11)-positive lymphocytes as determined by two-colour flow cytometry. Analysis of glycoproteins in the lysate prepared from NK cell...

journal_title：Glycoconjugate journal

authors： Harada K,Yamane H,Imai Y,Tsuji T,Toyoshima S,Osawa T

更新日期：1992-08-01 00:00:00

abstract：:Cholera remains to be a global health problem without suitable vaccines for endemic control or outbreak relief. Here we describe a new parenteral vaccine based on neoglyco-conjugate of synthetic fragments of O-specific polysaccharide (O-SP) of Vibrio cholerae O1, serotype Ogawa. Hexa-, octa- and decasaccharides of the...

journal_title：Glycoconjugate journal

authors： Ftacek P,Nelson V,Szu SC

更新日期：2013-12-01 00:00:00

abstract：:N-acetylglucosaminyltransferase (GnT)-IV a is a key enzyme that catalyzes the formation of the GlcNAC β1-4 branch on the core structure of complex N-Glycans, which is the common substrate for other N-acetylglucosaminyltransferases, such as GnT-III and GnT-V. Our recent study indicates that the expression of GnT-IVa in...

journal_title：Glycoconjugate journal

authors： Fan J,Wang S,Yu S,He J,Zheng W,Zhang J

更新日期：2012-08-01 00:00:00

abstract：:Two rhamnobiose-lipid preparations have been studied by fast atom bombardment (FAB) tandem mass spectrometry. The principal rhamnobiose-lipids contain the beta-hydroxydecanoyl-beta-hydroxydecanoate Rha-Rha-C10-C10 and the beta-hydroxytetradecanoyl-beta-hydroxytetradecanoate Rha-Rha-C14-C14. Both preparations contain m...

journal_title：Glycoconjugate journal

authors： Manso Pajarron A,De Koster CG,Heerma W,Schmidt M,Haverkamp J

更新日期：1993-06-01 00:00:00

abstract：:A monoclonal antibody (mAb 8281) specific for the terminal beta-galactose (beta Gal) of glycosphingolipids (GSL) and glycoproteins was produced from mice immunized with lipid extract from fresh acute lymphocytic leukemia (ALL) cells. Immuno-thin layer chromatography (ITLC) and competition assays with purified neutral ...

journal_title：Glycoconjugate journal

authors： Kalisiak A,Oosterwijk E,Minniti JG,Old LJ,Scheinberg DA

更新日期：1991-02-01 00:00:00

abstract：:In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatogr...

journal_title：Glycoconjugate journal

authors： Liu C,Ji HY,Wu P,Yu J,Liu AJ

更新日期：2020-08-01 00:00:00

abstract：:The carbohydrate determinants, sialyl Lewis A and sialyl Lewis X, which are frequently expressed on human cancer cells, serve as ligands for a cell adhesion molecule of the selectin family, E-selectin, which is expressed on vascular endothelial cells. These carbohydrate determinants are involved in the adhesion of can...

journal_title：Glycoconjugate journal

authors： Kannagi R

更新日期：1997-08-01 00:00:00

abstract：:Heterologous complementation in yeast has been a successful tool for cloning and characterisation of genes from various organisms. Therefore we constructed conditionally lethal Saccharomyces cerevisiae strains by replacing the endogenous promoter from the genes of interest (glycosyltransferases) by the stringently reg...

journal_title：Glycoconjugate journal

authors： Mazhari-Tabrizi R,Blank M,Mumberg D,Funk M,Schwarz RT,Eckert V

更新日期：1999-11-01 00:00:00

abstract：:Exposure of neuronal cells to nanomolar concentrations of oligosaccharide portions of ganglioside GM2 and GT1b stimulates cAMP-dependent protein kinase (PKA) Ca2+/calmodulin-dependent protein kinase II (CaMKII), respectively, in a few seconds suggesting the presence of glyco-receptor-like molecules on the surface of t...

journal_title：Glycoconjugate journal

authors： Higashi H,Chen NH

更新日期：2004-01-01 00:00:00

abstract：:Based on the O-specific polysaccharides of the lipopolysaccharides (O-polysaccharides, O-antigens), strains of a clonal species Escherichia coli are classified into 184 O serogroups. In this work, structures of the O-polysaccharides of E. coli O69 and O146 were elucidated and gene clusters for their biosynthesis were ...

journal_title：Glycoconjugate journal

authors： Knirel YA,Guo X,Senchenkova SN,Perepelov AV,Liu B,Shashkov AS

更新日期：2017-02-01 00:00:00

abstract：:The structural diversity and localization of cell surface glycosphingolipids (GSLs), including gangliosides, in glycolipid-enriched microdomains (GEMs, also known as lipid rafts) render them ideally suited to play important roles in mediating intercellular recognition, interactions, adhesion, receptor function, and si...

journal_title：Glycoconjugate journal

authors： Itokazu Y,Tsai YT,Yu RK

更新日期：2017-12-01 00:00:00

abstract：:A non-enzymatic reaction between sugars or aldehydes and the amino groups of proteins, lipids and nucleic acids contributes to the aging of macromolecules, which could impair their structural integrity and function. This process begins with the conversion of reversible Schiff base adducts, and then to more stable, cov...

journal_title：Glycoconjugate journal

authors： Yamagishi S,Taguchi K,Fukami K

更新日期：2016-08-01 00:00:00

abstract：:Synthetic thioglycoside-analogs of gangliosides such as Neu5Ac alpha(2-S-6)Glc beta(1-1)Ceramide (1) and the GM3 analog Neu5Ac alpha(2-S-6)Gal beta(1-4)Glc beta(1-1)Ceramide (2), competitively inhibited GM3 hydrolysis by the sialidase of different subtypes of human and animal influenza viruses with an apparent Ki valu...

journal_title：Glycoconjugate journal

authors： Suzuki Y,Sato K,Kiso M,Hasegawa A

更新日期：1990-01-01 00:00:00

abstract：:Transmannosylation from mannotriose (Man beta 1-4Man beta 1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue of N,N'-diacetylchitobiose was regioselectively induced through the use of beta-D-mannanase from Aspergillus niger. The enzyme formed the trisaccharide Man beta 1-4GlcNAc beta 1-4GlcN...

journal_title：Glycoconjugate journal

authors： Usui T,Suzuki M,Sato T,Kawagishi H,Adachi K,Sano H

更新日期：1994-04-01 00:00:00

abstract：:The expression of neutral glycosphingolipids and gangliosides has been studied in human skeletal and heart muscle using indirect immunofluorescence microscopy. Transversal and longitudinal cryosections were immunostained with specific monoclonal and polyclonal antibodies against the neural glycosphingolipids lactosylc...

journal_title：Glycoconjugate journal

authors： Cacić M,Müthing J,Kracun I,Neumann U,Weber-Schürholz S

更新日期：1994-10-01 00:00:00

abstract：:Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A numbe...

journal_title：Glycoconjugate journal

authors： Nemčovičová I,Šesták S,Rendić D,Plšková M,Mucha J,Wilson IB

更新日期：2013-12-01 00:00:00

abstract：:Syndecan-4 is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family. Syndecan-4-deficient [(Synd4(-/-)] mice were produced to clarify the in vivo role of syndecan-4. Synd4(-/-) mice were more susceptible to kappa-carrageenan-induced nephropathy, and the placental labyrinth from the deficient em...

journal_title：Glycoconjugate journal

authors： Ishiguro K,Kojima T,Muramatsu T

更新日期：2002-05-01 00:00:00

abstract：:Over the past two decades, sugar chain structures of human chorionic gonadotropin (hCG) produced in healthy people, three types of trophoblastic disease, and some types of cell lines have been analyzed. The abnormal biantennary structure of hCG is a good marker for the diagnosis of malignant choriocarcinoma. In spite ...

journal_title：Glycoconjugate journal

authors： Takamatsu S,Katsumata T,Inoue N,Watanabe T,Fujibayashi Y,Takeuchi M

更新日期：2004-01-01 00:00:00

abstract：:Human skin fibroblasts were incubated with a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU), in the presence or absence of tunicamycin. The xyloside-initiated glycosaminoglycans (GAG-MUs) were isolated from the culture medium, and their structures characterized. When the cells were incubated with ...

journal_title：Glycoconjugate journal

authors： Takagaki K,Tazawa T,Munakata H,Nakamura T,Endo M

更新日期：1998-05-01 00:00:00

abstract：:UDP-GlcNAc:Man alpha 1-3R beta 1-2-N-acetylglucosaminyltransferase I (GlcNAc-T I; EC 2.4.1.101) catalyses the conversion of [Man alpha 1-6(Man alpha 1-3)Man alpha 1-6][Man alpha 1-3]Man beta-O-R to [Man alpha 1-6(Man alpha 1-3)Man alpha 1-6] [GlcNAc beta 1-2Man alpha 1-3]Man beta-O-R (R = 1-4GlcNAc beta 1-4GlcNAc- Asn...

journal_title：Glycoconjugate journal

authors： Reck F,Springer M,Meinjohanns E,Paulsen H,Brockhausen I,Schachter H

更新日期：1995-12-01 00:00:00

abstract：:Oxygen-requiring enzymes, such as Δ4-desaturase (dihydroceramide desaturase), sphingolipid Δ4-desaturase/C-4-hydroxylase, and fatty acid 2-hydroxylase are involved in ceramide synthesis. We prepared free ceramides, sphingomyelins and glycosphingolipids (GSLs) from cancer cells cultivated under conditions of normoxia a...

journal_title：Glycoconjugate journal

authors： Tanaka K,Tamiya-Koizumi K,Yamada M,Murate T,Kannagi R,Kyogashima M

更新日期：2015-11-01 00:00:00