Developmental diseases caused by impaired nucleotide sugar transporters.

abstract：:Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and hFucTVI: Asn46 and Asn91...

journal_title：Glycoconjugate journal

authors： Christensen LL,Bross P,Ørntoft TF

更新日期：2000-12-01 00:00:00

abstract：:Human skin fibroblasts were incubated with a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU), in the presence or absence of tunicamycin. The xyloside-initiated glycosaminoglycans (GAG-MUs) were isolated from the culture medium, and their structures characterized. When the cells were incubated with ...

journal_title：Glycoconjugate journal

authors： Takagaki K,Tazawa T,Munakata H,Nakamura T,Endo M

更新日期：1998-05-01 00:00:00

abstract：:UDP-2-(2-ketopropyl)galactose (1) has been utilized as a valuable probe for profiling proteins modified by O-GlcNAc. In this work, we developed a protocol for efficient synthesis of 1. Thus, 2-methallylgalactose derivative 11, a synthetic intermediate for the compound 1, was prepared by stereoselective iodination and ...

journal_title：Glycoconjugate journal

authors： Sakamoto Y,Ohta T,Ito Y

更新日期：2015-10-01 00:00:00

abstract：:We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction ...

journal_title：Glycoconjugate journal

authors： Kinsella L,Chen HL,Smith JA,Rudland PS,Fernig DG

更新日期：1998-04-01 00:00:00

abstract：:Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectin-mediated cell adhesion to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures and subsequent binding of ECM glycoproteins. First, we synthesized be...

journal_title：Glycoconjugate journal

authors： Sauerzapfe B,Krenek K,Schmiedel J,Wakarchuk WW,Pelantová H,Kren V,Elling L

更新日期：2009-02-01 00:00:00

abstract：:Recently, we demonstrated that the human xylosyltransferase II (XT-II) has enzymatic activity and is able to catalyze the initial and rate-limiting step in the biosynthesis of glycosaminoglycans (GAGs) like chondroitin and dermatan sulfate, as well as heparan sulfate and heparin. Therefore, this enzyme also very likel...

journal_title：Glycoconjugate journal

authors： Müller B,Prante C,Knabbe C,Kleesiek K,Götting C

更新日期：2013-04-01 00:00:00

abstract：:In this study we report on the first mass spectrometric (MS) investigation of gangliosides and preliminary assessment of the expression and structure in normal fetal neocortex in early developmental stages: 14th (Neo14) and 16th (Neo16) gestational weeks. Ganglioside analysis was carried out using a hybrid quadrupole ...

journal_title：Glycoconjugate journal

authors： Ghiulai RM,Sarbu M,Vukelić Ž,Ilie C,Zamfir AD

更新日期：2014-04-01 00:00:00

abstract：:The TF, Tn, and SiaTn glycotopes are frequently expressed in cancer-associated mucins. Antibodies to these glycotopes were found in human serum. A set of polyacrylamide (PAA)--based glycoconjugates was applied to the direct and competitive enzyme-linked immunosorbent assays (ELISA) to characterize the specificity of s...

journal_title：Glycoconjugate journal

authors： Smorodin EP,Kurtenkov OA,Sergeyev BL,Pazynina GV,Bovin NV

更新日期：2004-01-01 00:00:00

abstract：:Carbohydrate-carbohydrate interaction is a reliable and versatile mechanism for cell adhesion and recognition. Glycosphingolipid (GSL) clusters at the cell membrane are mainly involved in this interaction. To investigate carbohydrate-carbohydrate interaction an integrated strategy (Glyconanotechnology) was developed. ...

journal_title：Glycoconjugate journal

authors： de la Fuente JM,Penadés S

更新日期：2004-01-01 00:00:00

abstract：:Rice (Oryza sativa) expresses different putative carbohydrate-binding proteins belonging to the class of lectins containing an Euonymus lectin (EUL)-related domain, one of them being OrysaEULS2. The OrysaEULS2 sequence consists of a 56 amino acid N-terminal domain followed by the EUL sequence. In this paper the origin...

journal_title：Glycoconjugate journal

authors： Al Atalah B,Rougé P,Smith DF,Proost P,Lasanajak Y,Van Damme EJ

更新日期：2012-10-01 00:00:00

abstract：:The conformational space of methyl 6-O-[(R)- and (S)-1-carboxyethyl]-alpha-D-galactopyranoside has been investigated. A grid search employing energy minimization at each grid point over the three major degrees of freedom, namely phi, psi and omega, identified low energy regions. The R-isomer shows five low energy conf...

journal_title：Glycoconjugate journal

authors： Stenutz R,Widmalm G

更新日期：1997-12-01 00:00:00

abstract：:Rotaviruses are the leading cause of childhood diarrhea. The entry of rotaviruses into the host cell is a complex process that includes several interactions of the outer layer proteins of the virus with different cell surface molecules. The fact that neuraminidase treatment of the cells, or preincubation of the virus ...

journal_title：Glycoconjugate journal

authors： Isa P,Arias CF,López S

更新日期：2006-02-01 00:00:00

abstract：:Humans, in contrast to other mammals, do not synthesize N-glycolyl-neuraminic acid (Neu5Gc) due to a deletion in the gene (cmah) encoding the enzyme responsible for this conversion, the cytidine monophospho-N-acetyl-neuraminic acid hydroxylase (CMP-Neu5Ac hydroxylase). The detection of considerable amounts of Neu5Gc-s...

journal_title：Glycoconjugate journal

authors： Casadesús AV,Fernández-Marrero Y,Clavell M,Gómez JA,Hernández T,Moreno E,López-Requena A

更新日期：2013-10-01 00:00:00

abstract：:Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embr...

journal_title：Glycoconjugate journal

authors： Toyoda H,Nagai Y,Kojima A,Kinoshita-Toyoda A

更新日期：2017-04-01 00:00:00

abstract：:Heteropolysaccarides were isolated from the Korean medicinal plant, Phellodendri cortex (Hwangbek), by hot water and alkali extractions. The extracted polysaccharides were fractionated into eight fractions and they are mainly composed of D-N-acetylglucosamine, D-galactose, D-mannose, and D-glucose. Among the polysacch...

journal_title：Glycoconjugate journal

authors： Park JI,Shim JK,Do JW,Kim SY,Seo EK,Kwon HJ,Lee TK,Kim JK,Choi DY,Kim CH

更新日期：1999-03-01 00:00:00

abstract：:Chondroitin sulfate (CS) and dermatan sulfate (DS) interact with various extracellular molecules such as growth factors, cytokines/chemokines, neurotrophic factors, morphogens, and viral proteins, thereby playing roles in a variety of biological processes including cell adhesion, proliferation, tissue morphogenesis, n...

journal_title：Glycoconjugate journal

authors： Mizumoto S,Fongmoon D,Sugahara K

更新日期：2013-08-01 00:00:00

abstract：:Human Gb3/CD77 synthase (α1,4-galactosyltransferase) is the only known glycosyltransferase that changes acceptor specificity because of a point mutation. The enzyme, encoded by A4GALT locus, is responsible for biosynthesis of Gal(α1-4)Gal moiety in Gb3 (CD77, Pk antigen) and P1 glycosphingolipids. We showed before tha...

journal_title：Glycoconjugate journal

authors： Kaczmarek R,Mikolajewicz K,Szymczak K,Duk M,Majorczyk E,Krop-Watorek A,Buczkowska A,Czerwinski M

更新日期：2016-12-01 00:00:00

abstract：:O-Glycopyranosyl-N-hydroxysuccinimides of glucose and lactose have been synthesized. Peracetylated sugars, acetobromo sugars and benzoylated thioethyl glycosides have been used as precursors. With boron trifluoride etherate in dichloromethane, peracetylated sugars gave predominantly the beta anomer. Trimethylsilyl tri...

journal_title：Glycoconjugate journal

authors： Andersson M,Oscarson S

更新日期：1992-06-01 00:00:00

abstract：:Various oligosaccharides containing galactose(s) and one glucosamine (or N-acetylglucosamine) residues with beta1-4, alpha1-6 and beta1-6 glycosidic bond were synthesized; Galbeta1-4GlcNH(2), Galalpha1-6GlcNH(2), Galalpha1-6GlcNAc, Galbeta1-6GlcNH(2), Galbeta1-4Galbeta1-4GlcNH(2) and Galbeta1-4Galbeta1-4GlcNAc. Galalp...

journal_title：Glycoconjugate journal

authors： Hosomi O,Misawa Y,Takeya A,Matahira Y,Sugahara K,Kubohara Y,Yamakura F,Kudo S

更新日期：2009-02-01 00:00:00

abstract：:The Maillard reaction was discovered in 1912 by Louis C. Maillard when he observed the browning phenomena with aroma formation in a heated solution containing a sugar and an amino acid. The Maillard reaction starts from the reactions between carbonyl groups of various sugars and amino groups of amino acids/ proteins, ...

journal_title：Glycoconjugate journal

authors： Murata M

更新日期：2020-09-10 00:00:00

abstract：:The expression of neutral glycosphingolipids and gangliosides has been studied in human skeletal and heart muscle using indirect immunofluorescence microscopy. Transversal and longitudinal cryosections were immunostained with specific monoclonal and polyclonal antibodies against the neural glycosphingolipids lactosylc...

journal_title：Glycoconjugate journal

authors： Cacić M,Müthing J,Kracun I,Neumann U,Weber-Schürholz S

更新日期：1994-10-01 00:00:00

abstract：:Homology searches indicated that up to five class I α-mannosidases (glycohydrolase family 47) and eight class II α-mannosidases (glycohydrolase family 38) are encoded by the fruitfly (Drosophila melanogaster) genome. Selected example mannosidases were expressed in secreted form using the yeast Pichia pastoris. A numbe...

journal_title：Glycoconjugate journal

authors： Nemčovičová I,Šesták S,Rendić D,Plšková M,Mucha J,Wilson IB

更新日期：2013-12-01 00:00:00

abstract：:Glycans serve as important regulators of antibody activities and half-lives. IgE is the most heavily glycosylated antibody, but in comparison to other antibodies little is known about its glycan structure function relationships. We therefore describe the site specific IgE glycosylation from a patient with a novel hype...

journal_title：Glycoconjugate journal

authors： Wu G,Hitchen PG,Panico M,North SJ,Barbouche MR,Binet D,Morris HR,Dell A,Haslam SM

更新日期：2016-06-01 00:00:00

abstract：:Heterologous complementation in yeast has been a successful tool for cloning and characterisation of genes from various organisms. Therefore we constructed conditionally lethal Saccharomyces cerevisiae strains by replacing the endogenous promoter from the genes of interest (glycosyltransferases) by the stringently reg...

journal_title：Glycoconjugate journal

authors： Mazhari-Tabrizi R,Blank M,Mumberg D,Funk M,Schwarz RT,Eckert V

更新日期：1999-11-01 00:00:00

abstract：:Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...

journal_title：Glycoconjugate journal

authors： Mandal C,Srinivasan GV,Chowdhury S,Chandra S,Mandal C,Schauer R,Mandal C

更新日期：2009-01-01 00:00:00

abstract：:The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...

journal_title：Glycoconjugate journal

authors： Henry S,Mollicone R,Fernandez P,Samuelsson B,Oriol R,Larson G

更新日期：1996-12-01 00:00:00

abstract：:Comparing the properties of 'young' and senescent ('aged') O+ erythrocytes isolated by applying ultracentrifugation in a self-forming Percoll gradient, we demonstrate that the sialic acids of membrane glycoconjugates control the life span of erythrocytes and that the desialylation of glycans is responsible for the cle...

journal_title：Glycoconjugate journal

authors： Bratosin D,Mazurier J,Debray H,Lecocq M,Boilly B,Alonso C,Moisei M,Motas C,Montreuil J

更新日期：1995-06-01 00:00:00

abstract：:Egg yolk, a large proportion of the egg, was studied for the preparation of N-acetylneuraminic acid (Neu5Ac). The delipidated hen egg yolk (DEY; 500 kg containing 0.2% w/w, Neu5Ac) was hydrolysed with HCl (pH 1.4) at 80 degrees C and neutralized with NaOH (pH 6.0). The mixture was filtered and electrodialysed until th...

journal_title：Glycoconjugate journal

authors： Koketsu M,Juneja LR,Kawanami H,Kim M,Yamamoto T

更新日期：1992-04-01 00:00:00

abstract：:Galectins are animal lectins that can specifically bind beta-galactosides. Thirteen galectins have already been described. This review focuses on a specific member of this family: galectin-8. This galectin was discovered in prostate cancer cells eight years ago and has been studied extensively in the last few years. T...

journal_title：Glycoconjugate journal

authors： Bidon-Wagner N,Le Pennec JP

更新日期：2002-01-01 00:00:00

abstract：:Infectious disease, commonly caused by bacterial pathogens, is now the world's leading cause of premature death and third overall cause behind cardiovascular disease and cancer. Urinary Tract Infection (UTI), caused by E. coli bacteria, is a very common bacterial infection, a majority in women (85%) and may result in ...

journal_title：Glycoconjugate journal

authors： Basu M,Seggerson S,Henshaw J,Jiang J,del A Cordona R,Lefave C,Boyle PJ,Miller A,Pugia M,Basu S

更新日期：2004-01-01 00:00:00