Abstract:
:Chondroitin sulfate (CS) and dermatan sulfate (DS) interact with various extracellular molecules such as growth factors, cytokines/chemokines, neurotrophic factors, morphogens, and viral proteins, thereby playing roles in a variety of biological processes including cell adhesion, proliferation, tissue morphogenesis, neurite outgrowth, infections, and inflammation/leukocyte trafficking. CS/DS are modified with sulfate groups at C-2 of uronic acid residues as well as C-4 and/or C-6 of N-acetyl-D-galactosamine residues, yielding enormous structural diversity, which enables the binding with numerous proteins. We have demonstrated that highly sulfated CS-E from squid cartilage, for example, interacts with heparin-binding proteins including midkine, pleiotrophin, and fibroblast growth factors expressed in brain with high affinity (Kd values in the nM range). Here, we analyzed the binding of CS and DS, which have a relatively low degree of sulfation and have been widely used as a nutraceutical and a drug for osteoarthritis etc., with a number of heparin-binding neurotrophic factors/cytokines using surface plasmon resonance (SPR) and structurally characterized the CS/DS chains. SPR showed that relatively low sulfated CS-A, DS, and CS-C also bound with significant affinity to midkine, pleiotrophin, hepatocyte growth factor, monokine-induced by interferon-γ, and stromal cell derived factor-1β, although the binding was less intense than that with highly sulfated CS-D and CS-E. These findings suggest that even low sulfated CS and/or DS chains may contain binding domains, which include fine sugar sequences with specific sulfation patterns, and that sugar sequences, conformations and electrostatic potential are more important than the simple degree of sulfation represented by disaccharide composition.
journal_name
Glycoconj Jjournal_title
Glycoconjugate journalauthors
Mizumoto S,Fongmoon D,Sugahara Kdoi
10.1007/s10719-012-9463-5subject
Has Abstractpub_date
2013-08-01 00:00:00pages
619-32issue
6eissn
0282-0080issn
1573-4986journal_volume
30pub_type
杂志文章abstract::Amphipathic sulpholipids have been shown to inhibit pancreatic serine proteases due to their detergent-like properties. To evaluate the structural requirement for this inhibitory activity, we examined the effects of various derivatives of sulphoglycolipids, some of which were prepared by deacylation with sphingolipid ...
journal_title:Glycoconjugate journal
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journal_title:Glycoconjugate journal
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journal_title:Glycoconjugate journal
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journal_title:Glycoconjugate journal
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更新日期:2004-01-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2012-04-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2001-03-01 00:00:00
abstract::Galectin-1 has demonstrated a diverse range of activities in relation to cell survival and proliferation. In different circumstances, it acts as a mitogen, as an inhibitor of cell proliferation, and as a promoter of cellular apoptosis. Many of these activities, particularly the mitogenic and apoptotic responses, follo...
journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
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更新日期:2002-01-01 00:00:00
abstract::In aging and diabetes, glycation of collagen molecules leads to the formation of cross-links that could alter the surface charge on collagen fibrils, and hence affect the properties and correct functioning of a number of tissues. The electron-optical stain phosphotungstic acid (PTA) binds to positively charged amino a...
journal_title:Glycoconjugate journal
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journal_title:Glycoconjugate journal
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更新日期:2016-12-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2018-12-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2006-11-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2018-12-01 00:00:00
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journal_title:Glycoconjugate journal
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journal_title:Glycoconjugate journal
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更新日期:2005-03-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2018-04-01 00:00:00
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更新日期:2004-01-01 00:00:00
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journal_title:Glycoconjugate journal
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更新日期:2001-10-01 00:00:00
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journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
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更新日期:1997-08-01 00:00:00
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journal_title:Glycoconjugate journal
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journal_title:Glycoconjugate journal
pub_type: 杂志文章,评审
doi:10.1007/s10719-006-9023-y
更新日期:2007-04-01 00:00:00
abstract::The expression of neutral glycosphingolipids and gangliosides has been studied in human skeletal and heart muscle using indirect immunofluorescence microscopy. Transversal and longitudinal cryosections were immunostained with specific monoclonal and polyclonal antibodies against the neural glycosphingolipids lactosylc...
journal_title:Glycoconjugate journal
pub_type: 杂志文章
doi:10.1007/BF00731284
更新日期:1994-10-01 00:00:00
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journal_title:Glycoconjugate journal
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doi:10.1007/s10719-021-09972-0
更新日期:2021-01-28 00:00:00
abstract::The N-glycans from soluble extracts of ten pollens were examined. The pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase HPLC, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. In compar...
journal_title:Glycoconjugate journal
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更新日期:1998-11-01 00:00:00