Specificity of human anti-carbohydrate IgG antibodies as probed with polyacrylamide-based glycoconjugates.

abstract：:The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy. hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB). The beta-hCG protein is encoded by CGB3, CGB5, CGB7 and CGB8 genes. CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas...

journal_title：Glycoconjugate journal

authors： Biskup K,Blanchard V,Castillo-Binder P,Alexander H,Engeland K,Schug S

更新日期：2020-10-01 00:00:00

abstract：:Two rhamnobiose-lipid preparations have been studied by fast atom bombardment (FAB) tandem mass spectrometry. The principal rhamnobiose-lipids contain the beta-hydroxydecanoyl-beta-hydroxydecanoate Rha-Rha-C10-C10 and the beta-hydroxytetradecanoyl-beta-hydroxytetradecanoate Rha-Rha-C14-C14. Both preparations contain m...

journal_title：Glycoconjugate journal

authors： Manso Pajarron A,De Koster CG,Heerma W,Schmidt M,Haverkamp J

更新日期：1993-06-01 00:00:00

abstract：:Galectin-1 has demonstrated a diverse range of activities in relation to cell survival and proliferation. In different circumstances, it acts as a mitogen, as an inhibitor of cell proliferation, and as a promoter of cellular apoptosis. Many of these activities, particularly the mitogenic and apoptotic responses, follo...

journal_title：Glycoconjugate journal

authors： Scott K,Weinberg C

更新日期：2002-01-01 00:00:00

abstract：:Blood group oligosaccharides are one of the most clinically important antigen families and they may also act as secondary ligands for bacterial toxins from Escherichia coli and Vibrio cholerae. Herein we report the synthesis of spacered (sp = CH2CH2CH2NH2) glycosides of A antigen {α-D-GalNAc-(l→3)-[α-L-Fuc-(l→2)]-β-D-...

journal_title：Glycoconjugate journal

authors： Karki G,Mishra VN,Mandal PK

更新日期：2016-02-01 00:00:00

abstract：:Based on the O-specific polysaccharides of the lipopolysaccharides (O-polysaccharides, O-antigens), strains of a clonal species Escherichia coli are classified into 184 O serogroups. In this work, structures of the O-polysaccharides of E. coli O69 and O146 were elucidated and gene clusters for their biosynthesis were ...

journal_title：Glycoconjugate journal

authors： Knirel YA,Guo X,Senchenkova SN,Perepelov AV,Liu B,Shashkov AS

更新日期：2017-02-01 00:00:00

abstract：:The main component of the ganglioside(1) mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay pro...

journal_title：Glycoconjugate journal

authors： Rizzo AM,Berra B,Rossi F,Guerra A,Gornati R,Bernardini G,Taki T,Kasama T,Mauri L,Sonnino S

更新日期：2002-01-01 00:00:00

abstract：:Lubrication of mammalian joints is mediated by lubricin, a product of megakaryocyte stimulating factor gene (MSF; GenBank accession #U70136) expression. Lubricin (M(r) approximately 240 kDa) is a mucinous glycoprotein which is 50% (w/w) post-translationally modified with beta(1-3)Gal-GalNAc incompletely capped with Ne...

journal_title：Glycoconjugate journal

authors： Jay GD,Harris DA,Cha CJ

更新日期：2001-10-01 00:00:00

abstract：:We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction ...

journal_title：Glycoconjugate journal

authors： Kinsella L,Chen HL,Smith JA,Rudland PS,Fernig DG

更新日期：1998-04-01 00:00:00

abstract：:We have previously detected an alkali-labile and developmentally regulated antigen in rat embryonic cerebral cortex, which may be 9-O-acetylsialylated GT3 ganglioside (Hirabayashi Y, Hirota M, Suzuki Y, Matsumoto M, Obata K, Ando S (1989) Neurosci Lett 106:193-98). In this study we established a mouse monoclonal antib...

journal_title：Glycoconjugate journal

authors： Zhang G,Ji L,Kurono S,Fujita SC,Furuya S,Hirabayashi Y

更新日期：1997-11-01 00:00:00

abstract：:To reveal the function of the carbohydrate portion of glycopeptides and glycoproteins, we chemo-enzymatically synthesized artificially N-glycosylated derivatives of eel calcitonin and studied their three-dimensional structure and biological activity. The CD and NMR spectra in trifluoroethanol-H(2)O solution showed tha...

journal_title：Glycoconjugate journal

authors： Tagashira M,Tanaka A,Hisatani K,Isogai Y,Hori M,Takamatsu S,Fujibayashi Y,Yamamoto K,Haneda K,Inazu T,Toma K

更新日期：2001-06-01 00:00:00

abstract：:The uncovering enzyme (UCE) removes N-acetylglucosamine from lysosomal enzymes to uncover the mannose 6-phosphate (Man-6-P) determinant necessary for targeting these enzymes to lysosomes. Failure to create the Man-6-P determinant is one cause of lysosomal storage diseases. Despite its medical importance, little struct...

journal_title：Glycoconjugate journal

authors： Wei Y,Yen TY,Cai J,Trent JO,Pierce WM,Young WW Jr

更新日期：2005-02-01 00:00:00

abstract：:Galectin-3 is a beta-galactoside-binding protein involved in immunomodulation, cell interactions, cancer progression, and pathogenesis of infectious organisms. We report the identification and characterization of galectin-3 in human semen. In the male reproductive tract, the approximately 30 kDa galectin-3 protein was...

journal_title：Glycoconjugate journal

authors： Jones JL,Saraswati S,Block AS,Lichti CF,Mahadevan M,Diekman AB

更新日期：2010-02-01 00:00:00

abstract：:UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II (GnT II; EC 2.4.1.143) is a medial-Golgi resident enzyme that catalyses an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides. Screening a cDNA library from Xenopus laevis ovary with a human ...

journal_title：Glycoconjugate journal

authors： Mucha J,Svoboda B,Kappel S,Strasser R,Bencur P,Fröhwein U,Schachter H,Mach L,Glössl J

更新日期：2002-03-01 00:00:00

abstract：:The novel glycosphingolipid, SEGLx (Galbeta1-4(Fucalpha1-3)Glcbeta1-3GalbetaCer), which was identified by us (Kawakami Y, et al. (1993) J Biochem 114: 677-83), shows a characteristic spectrum on 1H-NMR analysis, in which the anomeric proton resonances of a reducing end galactose and a glucose are split. To elucidate t...

journal_title：Glycoconjugate journal

authors： Kawakami J,Kawakami Y,Nakamura K,Kojima H,Ito S,Tamai Y

更新日期：1998-02-01 00:00:00

abstract：:We have previously shown that murine resident peritoneal macrophages (PEMs) are activated in response to uptake of oligomannose-coated liposomes (OMLs), leading to production of interleukin (IL)-12. To understand the mechanism of activation of PEMs by OMLs, in the present study we investigated the role of a mannose-bi...

journal_title：Glycoconjugate journal

authors： Kato C,Kojima N

更新日期：2010-07-01 00:00:00

abstract：:Oral exposure to lectins or the presence or absence of bacteria in the rat small intestine were shown by histological methods using anti-lectin antibodies or digoxigenin-labelled lectins to have major effects on the state of glycosylation of lumenal membranes and cytoplasmic glycoconjugates of epithelial cells. Taken ...

journal_title：Glycoconjugate journal

authors： Pusztai A,Ewen SW,Grant G,Peumans WJ,Van Damme EJ,Coates ME,Bardocz S

更新日期：1995-02-01 00:00:00

abstract：:Mammalian sulfoglycolipids are comprised of two major members, sulfatide (SO(3)-3Gal-ceramide) and seminolipid (SO(3)-3Gal-alkylacylglycerol). Sulfatide is abundant in the myelin sheath and seminolipid is expressed on the spermatogenic cells. Cerebroside sulfotransferase (CST)-deficient mice generated by gene targetin...

journal_title：Glycoconjugate journal

authors： Honke K,Zhang Y,Cheng X,Kotani N,Taniguchi N

更新日期：2004-01-01 00:00:00

abstract：:Type 1 ABO blood group antigens (peripheral core structure: Gal beta 1-3GlcNAc beta 1-R) are expressed mainly in endodermally-derived tissues, but are not synthesized in mesodermally-derived tissues. In the former tissues, H type 1 antigen is generated largely by alpha-2-L-fucosyltransferase encoded by secretor (Se) g...

journal_title：Glycoconjugate journal

authors： Tanegashima A,Nishi K,Fukunaga T,Rand S,Brinkmann B

更新日期：1996-08-01 00:00:00

abstract：:Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presen...

journal_title：Glycoconjugate journal

authors： Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Z

更新日期：2019-06-01 00:00:00

abstract：:Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...

journal_title：Glycoconjugate journal

authors： Mandal C,Srinivasan GV,Chowdhury S,Chandra S,Mandal C,Schauer R,Mandal C

更新日期：2009-01-01 00:00:00

abstract：:Fucosylated oligosaccharides have an important role in maintaining a healthy immune system and homeostatic gut microflora. This study employed a commercial β-galactosidase in the production of fucose-containing galacto-oligosaccharides (fGOS) from lactose and fucose. The production was optimized using experiment desig...

journal_title：Glycoconjugate journal

authors： Usvalampi A,Maaheimo H,Tossavainen O,Frey AD

更新日期：2018-02-01 00:00:00

abstract：:Proteoglycans contain a unique carbohydrate component, glycosaminoglycan, which consists of repeating, typically sulfated disaccharides, and is capable of interacting with diverse molecules. Specific, clustered arrangements of sulfate on the glycosaminoglycan backbone form binding sites for many biologically important...

journal_title：Glycoconjugate journal

authors： Yanagishita M,Podyma-Inoue KA,Yokoyama M

更新日期：2009-11-01 00:00:00

abstract：:Frog integumentary mucin B.1 (FIM-B.1) represents a polymorphic extracellular mosaic protein which contains tandemly arranged serine/threonine-rich modules as well as cysteine-rich domains. The latter are probably important for oligomerization of FIM-B.1 and have also been found in many proteins of the complement casc...

journal_title：Glycoconjugate journal

authors： Joba W,Hoffmann W

更新日期：1996-10-01 00:00:00

abstract：:Gastric carcinoma is a major cause of cancer death worldwide and, like most human cancers, probably develops after environmental insults acting on normal individuals and/or individuals with increased genetic susceptibility. Mucins are attractive molecules to study the relationship between genetics and environment beca...

journal_title：Glycoconjugate journal

authors： Carvalho F,Seruca R,David L,Amorim A,Seixas M,Bennett E,Clausen H,Sobrinho-Simões M

更新日期：1997-01-01 00:00:00

abstract：:The partitioning of the overall molecular charge distribution into atom centered monopole charges, while quantum mechanically ill-defined, is nevertheless a technique which finds applications in several broad classes of chemical problems. Charges derived from fits to electrostatic potentials have an intuitive appeal s...

journal_title：Glycoconjugate journal

authors： Carey C,Chirlian LE,Francl MM,Gange DM

更新日期：1997-06-01 00:00:00

abstract：:Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW approximately 32 kDa ...

journal_title：Glycoconjugate journal

authors： Raghav SK,Gupta B,Agrawal C,Saroha A,Das RH,Chaturvedi VP,Das HR

更新日期：2006-05-01 00:00:00

abstract：:Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present. Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great inter...

journal_title：Glycoconjugate journal

authors： Baerenfaenger M,Moritz M,Meyer B

更新日期：2019-02-01 00:00:00

abstract：:Heterologous complementation in yeast has been a successful tool for cloning and characterisation of genes from various organisms. Therefore we constructed conditionally lethal Saccharomyces cerevisiae strains by replacing the endogenous promoter from the genes of interest (glycosyltransferases) by the stringently reg...

journal_title：Glycoconjugate journal

authors： Mazhari-Tabrizi R,Blank M,Mumberg D,Funk M,Schwarz RT,Eckert V

更新日期：1999-11-01 00:00:00

abstract：:The human glioma D-54MG cell line grown in vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3'-isoLM1, an alteration that was do...

journal_title：Glycoconjugate journal

authors： Fredman P,Wikstrand CJ,Månsson JE,Reifenberger G,Bigner SH,Rasheed A,Svennerholm L,Bigner DD

更新日期：1996-06-01 00:00:00

abstract：:Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens o...

journal_title：Glycoconjugate journal

authors： Nakamura Y,Miyata Y,Matsuo T,Shida Y,Hakariya T,Ohba K,Taima T,Ito A,Suda T,Hakomori SI,Saito S,Sakai H

更新日期：2019-10-01 00:00:00