Abstract:
:Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presence within CCA, and their possible structural characteristics, were previously unknown. In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities. The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures. This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.
journal_name
Glycoconj Jjournal_title
Glycoconjugate journalauthors
Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Zdoi
10.1007/s10719-019-09868-0subject
Has Abstractpub_date
2019-06-01 00:00:00pages
211-218issue
3eissn
0282-0080issn
1573-4986pii
10.1007/s10719-019-09868-0journal_volume
36pub_type
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