Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.

Abstract:

:Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presence within CCA, and their possible structural characteristics, were previously unknown. In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities. The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures. This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.

journal_name

Glycoconj J

journal_title

Glycoconjugate journal

authors

Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Z

doi

10.1007/s10719-019-09868-0

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

211-218

issue

3

eissn

0282-0080

issn

1573-4986

pii

10.1007/s10719-019-09868-0

journal_volume

36

pub_type

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