Identification and biochemical characterization of WbwB, a novel UDP-Gal: Neu5Ac-R α1,4-galactosyltransferase from the intestinal pathogen Escherichia coli serotype O104.

abstract：:This review discusses the challenges involved in the characterization of the glycosylation of therapeutic glycoproteins. The focus is on methods that are most commonly used in regulatory filings and lot release testing of therapeutic glycoproteins. The different types of assays for carbohydrate analysis are reviewed, ...

journal_title：Glycoconjugate journal

authors： Higgins E

更新日期：2010-02-01 00:00:00

abstract：:Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embr...

journal_title：Glycoconjugate journal

authors： Toyoda H,Nagai Y,Kojima A,Kinoshita-Toyoda A

更新日期：2017-04-01 00:00:00

abstract：:Eggs from Amphibia are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of highly glycosylated mucin-type glycoproteins containing up to 60% of carbohydrates, which display a remarkable species-specificity. This material obtained from Triturus alpestris was submitt...

journal_title：Glycoconjugate journal

authors： Florea D,Maes E,Guérardel Y,Page A,Zanetta JP,Cogalniceanu D,Strecker G

更新日期：2006-07-01 00:00:00

abstract：:Comparing the properties of 'young' and senescent ('aged') O+ erythrocytes isolated by applying ultracentrifugation in a self-forming Percoll gradient, we demonstrate that the sialic acids of membrane glycoconjugates control the life span of erythrocytes and that the desialylation of glycans is responsible for the cle...

journal_title：Glycoconjugate journal

authors： Bratosin D,Mazurier J,Debray H,Lecocq M,Boilly B,Alonso C,Moisei M,Motas C,Montreuil J

更新日期：1995-06-01 00:00:00

abstract：:Both infiltrating leukocytes and soluble immunoglobulin form aggregates in synovial fluid during the inflammatory process in rheumatoid arthritis (RA). Some of these changes are probably mediated by the adhesion molecule, E-selectin, which increases its expression with disease activity. As glycosylation changes in IgG...

journal_title：Glycoconjugate journal

authors： Goodarzi MT,Axford JS,Varanasi SS,Alavi A,Cunnane G,Fitzgerald O,Turner GA

更新日期：1998-12-01 00:00:00

abstract：:Potato lectin (Solanum tuberosum agglutinin, STA) is an unusual glycoprotein containing approximately 50% carbohydrates by weight. Of the total carbohydrates, 92% is contributed by L: -arabinose, which are O-linked to hydroxyproline residues. The ferric chloride-orcinol assay (Bial's test), which is specific for pento...

journal_title：Glycoconjugate journal

authors： Pramod SN,Venkatesh YP

更新日期：2006-11-01 00:00:00

abstract：:The partial oligosaccharide sequences of glycoconjugates and the nature of their glycosidic linkages were investigated in normal human prostate, benign prostatic hyperplasia (BPH) and prostatic carcinoma by means of lectin histochemistry, using light microscopy and Western blot analysis. The labeling pattern of BPH di...

journal_title：Glycoconjugate journal

authors： Arenas MI,Romo E,de Gaspar I,de Bethencourt FR,Sánchez-Chapado M,Fraile B,Paniagua R

更新日期：1999-07-01 00:00:00

abstract：:Using a focused glycan-gene microarray, we compared the glycosyltransferase (GT) and sulfotransferase gene expression profiles of human monocytes, dendritic cells (DCs) and macrophages (Mphis), isolated or differentiated from the same donors. Microarray analysis indicated that monocytes express transcripts for a full ...

journal_title：Glycoconjugate journal

authors： Trottein F,Schaffer L,Ivanov S,Paget C,Vendeville C,Cazet A,Groux-Degroote S,Lee S,Krzewinski-Recchi MA,Faveeuw C,Head SR,Gosset P,Delannoy P

更新日期：2009-12-01 00:00:00

abstract：:Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...

journal_title：Glycoconjugate journal

authors： Mandal C,Srinivasan GV,Chowdhury S,Chandra S,Mandal C,Schauer R,Mandal C

更新日期：2009-01-01 00:00:00

abstract：:Jacalin and M. pomifera agglutinin are T-antigen specific lectins with alpha 4 beta 4 structures that show far greater micro-heterogeneity than plant lectins from other families, due to multiple genetic isoforms and post-translational processing. Electrospray mass spectrometry and combined liquid chromatography-electr...

journal_title：Glycoconjugate journal

authors： Young NM,Watson DC,Thibault P

更新日期：1995-04-01 00:00:00

abstract：:Mammalian milk/colostrum usually contains oligosaccharides along with the predominant disaccharide lactose. It has been found that the number and identity of these milk oligosaccharides varies among mammalian species. Oligosaccharides predominate over lactose in the milk/colostrum of Arctoidea species (Carnivora), whe...

journal_title：Glycoconjugate journal

authors： Mineguchi Y,Miyoshi M,Taufik E,Kawamura A,Asakawa T,Suzuki I,Souma K,Okubo M,Saito T,Fukuda K,Asakuma S,Urashima T

更新日期：2018-12-01 00:00:00

abstract：:The complex microenvironment that surrounds hematopoietic stem cells (HSCs) in the bone marrow niche involves different coordinated signaling pathways. The stem cells establish permanent interactions with distinct cell types such as mesenchymal stromal cells, osteoblasts, osteoclasts or endothelial cells and with secr...

journal_title：Glycoconjugate journal

authors： Papy-Garcia D,Albanese P

更新日期：2017-06-01 00:00:00

abstract：:Oral exposure to lectins or the presence or absence of bacteria in the rat small intestine were shown by histological methods using anti-lectin antibodies or digoxigenin-labelled lectins to have major effects on the state of glycosylation of lumenal membranes and cytoplasmic glycoconjugates of epithelial cells. Taken ...

journal_title：Glycoconjugate journal

authors： Pusztai A,Ewen SW,Grant G,Peumans WJ,Van Damme EJ,Coates ME,Bardocz S

更新日期：1995-02-01 00:00:00

abstract：:The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...

journal_title：Glycoconjugate journal

authors： Henry S,Mollicone R,Fernandez P,Samuelsson B,Oriol R,Larson G

更新日期：1996-12-01 00:00:00

abstract：:Non-acid and acid glycosphingolipids were isolated from feces of one litter of germ-free rats from day 17 to day 51. Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384-89] were also found in the feces of these germ...

journal_title：Glycoconjugate journal

authors： Larson G,Midtvedt T

更新日期：1989-01-01 00:00:00

abstract：:This review summarizes fifty years of carbohydrate chemistry, from studies of reaction mechanisms and development of synthetic methods to the design of specific compounds for investigations of cancer glycoproteins. Through these years of intensive research, many carbohydrate derivatives were shown to be biologically r...

journal_title：Glycoconjugate journal

authors： Paulsen H,Brockhausen I

更新日期：2001-11-01 00:00:00

abstract：:A novel cold-water-soluble polysaccharide (BEP), with a molecular weight of 6.0 × 106 Da, was isolated from Boletus edulis. BEP consists of galactose, glucose, xylose, mannose, glucuronic, and galacturonic acid in a ratio of 0.34:0.28:0.28:2.57:1.00:0.44. The IR results showed that BEP was an acid polysaccharide, cont...

journal_title：Glycoconjugate journal

authors： Meng T,Yu SS,Ji HY,Xu XM,Liu AJ

更新日期：2021-01-28 00:00:00

abstract：:Type 1 ABO blood group antigens (peripheral core structure: Gal beta 1-3GlcNAc beta 1-R) are expressed mainly in endodermally-derived tissues, but are not synthesized in mesodermally-derived tissues. In the former tissues, H type 1 antigen is generated largely by alpha-2-L-fucosyltransferase encoded by secretor (Se) g...

journal_title：Glycoconjugate journal

authors： Tanegashima A,Nishi K,Fukunaga T,Rand S,Brinkmann B

更新日期：1996-08-01 00:00:00

abstract：:The structures of the branched capsular polysaccharides of group B streptococcus type III (GBSIIIPS) and Streptococcus pneumoniae type 14 (Pn14PS) are identical apart from the (α2→3)-linked sialic acid in the side chains of GBSIIIPS. The present study tries to determine the minimal epitope in GBSIIIPS, using both a pa...

journal_title：Glycoconjugate journal

authors： Safari D,Dekker HA,Rijkers GT,van der Ende A,Kamerling JP,Snippe H

更新日期：2011-12-01 00:00:00

abstract：:Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. In this study, we used multiple lectin assays to analyze glycan patterns of serum GP73 and evaluated its diagnostic value for distinguishing hepatocellular carcinoma (HCC) from liver cirrhosis (LC). Firstly, ...

journal_title：Glycoconjugate journal

authors： Jiang K,Shang S,Li W,Guo K,Qin X,Zhang S,Liu Y

更新日期：2015-12-01 00:00:00

abstract：:Colominic acid is an alpha2,8-linked sialic acid polymer produced by Escherichia coli. We found that synthetic sulfated-colominic acids (SC) remarkably inhibited the cytotoxicity of bee and snake venom toward mouse fibroblast cells, but colominic acids showed no inhibition themselves, indicating the important role of ...

journal_title：Glycoconjugate journal

authors： Oda Y,Kinoshita M,Hamada K,Nakayama K,Ohta Y,Yamaguchi S,Tsukada Y,Kawai Y,Kakehi K

更新日期：1999-08-01 00:00:00

abstract：:Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-alpha-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1 and RGXT2 in Pi...

journal_title：Glycoconjugate journal

authors： Petersen BL,Egelund J,Damager I,Faber K,Jensen JK,Yang Z,Bennett EP,Scheller HV,Ulvskov P

更新日期：2009-12-01 00:00:00

abstract：:A new strategy for the fluorometric determination of glycosyltransferase activities is reported. The method involves dansyl chloride derivatization of the reduced form (pNH2phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO2phenyl). Focusing on the Golgi enzyme core 2 N-...

journal_title：Glycoconjugate journal

authors： Palmerini CA,Datti A,Vanderelst IE,Minuti L,Orlacchio A

更新日期：1996-08-01 00:00:00

abstract：:The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy. hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB). The beta-hCG protein is encoded by CGB3, CGB5, CGB7 and CGB8 genes. CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas...

journal_title：Glycoconjugate journal

authors： Biskup K,Blanchard V,Castillo-Binder P,Alexander H,Engeland K,Schug S

更新日期：2020-10-01 00:00:00

abstract：:As an adjunct to existing thin layer and column chromatographic methods for the identification of glycolipids a method that utilizes the high pH anion chromatographic (HPAEC) analysis of the oligosaccharides released from the glycolipids by endoglycoceramidase has been developed. Using a Dionex Carbo Pak PA1 column an...

journal_title：Glycoconjugate journal

authors： Ruan S,Lloyd KO

更新日期：1994-06-01 00:00:00

abstract：:Galectin-1 has demonstrated a diverse range of activities in relation to cell survival and proliferation. In different circumstances, it acts as a mitogen, as an inhibitor of cell proliferation, and as a promoter of cellular apoptosis. Many of these activities, particularly the mitogenic and apoptotic responses, follo...

journal_title：Glycoconjugate journal

authors： Scott K,Weinberg C

更新日期：2002-01-01 00:00:00

abstract：:To elucidate the biological significance of the lactosylceramide (LacCer) branching in glycosphingolipid (GSL) biosynthesis, we established ganglioside GM3- and lactosylsulfatide SM3-reconstituted cells by introducing the GM3 synthase gene and the sulfotransferase gene, respectively. In SM3-expressing cells, the reduc...

journal_title：Glycoconjugate journal

authors： Inokuchi J,Kabayama K,Uemura S,Igarashi Y

更新日期：2004-01-01 00:00:00

abstract：:Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens o...

journal_title：Glycoconjugate journal

authors： Nakamura Y,Miyata Y,Matsuo T,Shida Y,Hakariya T,Ohba K,Taima T,Ito A,Suda T,Hakomori SI,Saito S,Sakai H

更新日期：2019-10-01 00:00:00

abstract：:Mannose 6-phosphate receptor proteins (MPR 300 and 46) are type 1 transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. In a recent study we have purified both receptors from goat liver and raised antibodies. An ELISA method was developed that allowed quantification of both receptors in...

journal_title：Glycoconjugate journal

authors： Suresh K,Vegiraju SR,Nadimpalli SK

更新日期：2004-01-01 00:00:00

abstract：:Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and hFucTVI: Asn46 and Asn91...

journal_title：Glycoconjugate journal

authors： Christensen LL,Bross P,Ørntoft TF

更新日期：2000-12-01 00:00:00