N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.

abstract：:Galectins are animal lectins that can specifically bind beta-galactosides. Thirteen galectins have already been described. This review focuses on a specific member of this family: galectin-8. This galectin was discovered in prostate cancer cells eight years ago and has been studied extensively in the last few years. T...

journal_title：Glycoconjugate journal

authors： Bidon-Wagner N,Le Pennec JP

更新日期：2002-01-01 00:00:00

abstract：:The novel glycosphingolipid, SEGLx (Galbeta1-4(Fucalpha1-3)Glcbeta1-3GalbetaCer), which was identified by us (Kawakami Y, et al. (1993) J Biochem 114: 677-83), shows a characteristic spectrum on 1H-NMR analysis, in which the anomeric proton resonances of a reducing end galactose and a glucose are split. To elucidate t...

journal_title：Glycoconjugate journal

authors： Kawakami J,Kawakami Y,Nakamura K,Kojima H,Ito S,Tamai Y

更新日期：1998-02-01 00:00:00

abstract：:Chondroitin sulfate (CS) and dermatan sulfate (DS) interact with various extracellular molecules such as growth factors, cytokines/chemokines, neurotrophic factors, morphogens, and viral proteins, thereby playing roles in a variety of biological processes including cell adhesion, proliferation, tissue morphogenesis, n...

journal_title：Glycoconjugate journal

authors： Mizumoto S,Fongmoon D,Sugahara K

更新日期：2013-08-01 00:00:00

abstract：:Mannose 6-phosphate receptor proteins (MPR 300 and 46) are type 1 transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. In a recent study we have purified both receptors from goat liver and raised antibodies. An ELISA method was developed that allowed quantification of both receptors in...

journal_title：Glycoconjugate journal

authors： Suresh K,Vegiraju SR,Nadimpalli SK

更新日期：2004-01-01 00:00:00

abstract：:Matrix proteoglycans (PGs) have shown promise as biomarker in malignancies. We employed agarose gel eletrophoresis, quantitative real- time reverse transcription-polymerase chain reaction and immunohistochemistry to evaluate the content of sulfated glicosaminoglycans (chondroitin sulfate and heparan sulfate) and expre...

journal_title：Glycoconjugate journal

authors： Rangel MP,de Sá VK,Prieto T,Martins JRM,Olivieri ER,Carraro D,Takagaki T,Capelozzi VL

更新日期：2018-04-01 00:00:00

abstract：:The small keratan sulphate proteoglycan, fibromodulin, has been isolated from pooled human articular cartilage. The main chain repeat region and the chain caps from the attached N-linked keratan sulphate chains have been fragmented by keratanase II digestion, and the oligosaccharides generated have been reduced and is...

journal_title：Glycoconjugate journal

authors： Lauder RM,Huckerby TN,Nieduszynski IA

更新日期：1997-08-01 00:00:00

abstract：:Frog integumentary mucin B.1 (FIM-B.1) represents a polymorphic extracellular mosaic protein which contains tandemly arranged serine/threonine-rich modules as well as cysteine-rich domains. The latter are probably important for oligomerization of FIM-B.1 and have also been found in many proteins of the complement casc...

journal_title：Glycoconjugate journal

authors： Joba W,Hoffmann W

更新日期：1996-10-01 00:00:00

abstract：:A water-soluble polysaccharide CSPS-2B-2 with a molecular mass of 8.8 kDa, was obtained from the fruits of Capparis spinosa L. Chemical and NMR spectral analysis verified CSPS-2B-2 was a linear poly-(1-4)-α-D-galactopyranosyluronic acid in which 12.9±0.4% of carboxyl groups existed as methyl ester and 2.6±0.1% of D-Ga...

journal_title：Glycoconjugate journal

authors： Wang H,Wang H,Shi S,Duan J,Wang S

更新日期：2012-08-01 00:00:00

abstract：:N-Acetylneuraminic acid (a sialic acid) occurs mainly as a terminal substituent of oligosaccharides of glycoconjugates. Derivatives of neuraminic acid occur widely, substituted in the amino and hydroxy side chains, as well in the C-9 carbon skeleton. These derivatives are responsible for specific functions of sialic a...

journal_title：Glycoconjugate journal

authors： Stehling P,Gohlke M,Fitzner R,Reutter W

更新日期：1998-04-01 00:00:00

abstract：:Over the last few years enormous interest has been shown in the structures of the glycan moieties of various parasite surface glycoconjugates. Structures have been determined for the glyco-components of glycosylphosphatidylinositol (GPI) protein membrane anchors, for asparagine-linked oligosaccharides, and for the gly...

journal_title：Glycoconjugate journal

authors： Zamze S

更新日期：1991-12-01 00:00:00

abstract：:Blood group antigen-related oligosaccharides have been implicated in growth regulation, cell mobility control and adhesion; we are therefore interested in the localization of receptors for these oligosaccharides in tumour cells. Labelled neoglycoconjugates that carry synthetic sugar structures are suitable tools to de...

journal_title：Glycoconjugate journal

authors： Kayser K,Bovin NV,Zemlyanukhina TV,Donaldo-Jacinto S,Koopmann J,Gabius HJ

更新日期：1994-08-01 00:00:00

abstract：:GM3 ganglioside interacts specifically with complex-type N-linked glycans having multivalent GlcNAc termini, as shown for (1) and (2) below. (1) Oligosaccharides (OS) isolated from ConA-non-binding N-linked glycans of ovalbumin, whose structures were identified as penta-antennary complex-type with bisecting GlcNAc, ha...

journal_title：Glycoconjugate journal

authors： Yoon SJ,Nakayama K,Takahashi N,Yagi H,Utkina N,Wang HY,Kato K,Sadilek M,Hakomori SI

更新日期：2006-12-01 00:00:00

abstract：:A non-enzymatic reaction between sugars or aldehydes and the amino groups of proteins, lipids and nucleic acids contributes to the aging of macromolecules, which could impair their structural integrity and function. This process begins with the conversion of reversible Schiff base adducts, and then to more stable, cov...

journal_title：Glycoconjugate journal

authors： Yamagishi S,Taguchi K,Fukami K

更新日期：2016-08-01 00:00:00

abstract：:Sialosyl-Le(x) (SLex) and its positional isomer sialosyl-Le(a) are the epitopes recognized by the lectin domain of E- and P-selectins. Expression of SLex in polymorphonuclear leukocytes (PMN) plays an important role in recruitment of these cells at sites of inflammation through activation of selectins. We studied expr...

journal_title：Glycoconjugate journal

authors： Ito K,Handa K,Hakomori S

更新日期：1994-06-01 00:00:00

abstract：:In this study the comparative TLC immunostaining investigation of neutral GSLs and gangliosides from human skeletal and heart muscle is described. A panel of specific polyclonal and monoclonal antibodies as well as the GM1-specific choleragenoid were used for the overlay assays, combined with preceding neuraminidase t...

journal_title：Glycoconjugate journal

authors： Müthing J,Cacić M

更新日期：1997-01-01 00:00:00

abstract：:Syndecan-4 is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family. Syndecan-4-deficient [(Synd4(-/-)] mice were produced to clarify the in vivo role of syndecan-4. Synd4(-/-) mice were more susceptible to kappa-carrageenan-induced nephropathy, and the placental labyrinth from the deficient em...

journal_title：Glycoconjugate journal

authors： Ishiguro K,Kojima T,Muramatsu T

更新日期：2002-05-01 00:00:00

abstract：:The interaction between Urtica dioica agglutinin (UDA) and N-acetylglucosamine (GlcNAc) and its beta(1-4)-linked oligomers was studied by fluorescence titration and isothermal titration microcalorimetry. UDA possesses one significant binding site that can be measured calorimetrically. This site is composed of three su...

journal_title：Glycoconjugate journal

authors： Lee RT,Gabius HJ,Lee YC

更新日期：1998-07-01 00:00:00

abstract：:A new strategy for the fluorometric determination of glycosyltransferase activities is reported. The method involves dansyl chloride derivatization of the reduced form (pNH2phenyl) of a hydrophobic, aglycon moiety covalently linked to a number of acceptor substrates (pNO2phenyl). Focusing on the Golgi enzyme core 2 N-...

journal_title：Glycoconjugate journal

authors： Palmerini CA,Datti A,Vanderelst IE,Minuti L,Orlacchio A

更新日期：1996-08-01 00:00:00

abstract：:The mechanism of interleukin-3 (IL-3) independent cell growth and of IL-3-associated ganglioside expression was analysed using the IL-3 dependent murine myelogenous leukaemia cell line NFS60-I7 and IL-3 gene-transfected sublines. The transfected cell lines showed autonomous cell growth, tumorigenicity, and IL-3 associ...

journal_title：Glycoconjugate journal

authors： Nakamura M,Kirito K,Tsunoda A,Hara K,Furukawa Y,Saito M

更新日期：1996-04-01 00:00:00

abstract：:A straight forward synthesis of a heptasaccharide part of the cell-wall lipopolysaccharide of Mycobacterium gordonae strain 990, known to have antigenicity, has been achieved in excellent yield. Judicious choice of protecting groups in the intermediates played a significant role throughout the synthesis. Most of the i...

journal_title：Glycoconjugate journal

authors： Panchadhayee R,Kumar Misra A

更新日期：2008-12-01 00:00:00

abstract：:Fractions were prepared from the water-soluble components of Aspergillus fumigatus mycelium either by lectin-affinity chromatography or salt precipitation. While they varied considerably in their amino-acid composition, each contained a preponderance of aspartic and glutamic acids. 13C-NMR spectroscopy of these fracti...

journal_title：Glycoconjugate journal

authors： Hearn VM,Griffiths BL,Gorin PA

更新日期：1989-01-01 00:00:00

abstract：:The SewA385T mutation of the FUT2 gene was found to correlate with both the erthrocyte Le(a + b+) and/or salivary ABH partial-secretor phenotypes of Polynesians. Constructs with FUT1 and FUT2 wild type genes, and the FUT2 SewA385T, seG428A and seC571T mutated alleles, were cloned into pcDNAI, and expressed in COS-7 ce...

journal_title：Glycoconjugate journal

authors： Henry S,Mollicone R,Fernandez P,Samuelsson B,Oriol R,Larson G

更新日期：1996-12-01 00:00:00

abstract：:We analyzed surface glycoproteins of human natural killer (NK) cells by utilizing lectins. Among the lectins tested, wheat germ agglutinin (WGA) was found to bind preferentially to CD16(Leu11)-positive lymphocytes as determined by two-colour flow cytometry. Analysis of glycoproteins in the lysate prepared from NK cell...

journal_title：Glycoconjugate journal

authors： Harada K,Yamane H,Imai Y,Tsuji T,Toyoshima S,Osawa T

更新日期：1992-08-01 00:00:00

abstract：:C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excret...

journal_title：Glycoconjugate journal

authors： Hossain TJ,Manabe S,Ito Y,Iida T,Kosono S,Ueda K,Hosomi A,Inoue D,Suzuki T

更新日期：2018-04-01 00:00:00

abstract：:Gastric carcinoma is a major cause of cancer death worldwide and, like most human cancers, probably develops after environmental insults acting on normal individuals and/or individuals with increased genetic susceptibility. Mucins are attractive molecules to study the relationship between genetics and environment beca...

journal_title：Glycoconjugate journal

authors： Carvalho F,Seruca R,David L,Amorim A,Seixas M,Bennett E,Clausen H,Sobrinho-Simões M

更新日期：1997-01-01 00:00:00

abstract：:Cholera remains to be a global health problem without suitable vaccines for endemic control or outbreak relief. Here we describe a new parenteral vaccine based on neoglyco-conjugate of synthetic fragments of O-specific polysaccharide (O-SP) of Vibrio cholerae O1, serotype Ogawa. Hexa-, octa- and decasaccharides of the...

journal_title：Glycoconjugate journal

authors： Ftacek P,Nelson V,Szu SC

更新日期：2013-12-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids. The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients wi...

journal_title：Glycoconjugate journal

authors： Sturiale L,Bianca S,Garozzo D,Terracciano A,Agolini E,Messina A,Palmigiano A,Esposito F,Barone C,Novelli A,Fiumara A,Jaeken J,Barone R

更新日期：2019-12-01 00:00:00

abstract：:Two azidohexasaccharide building blocks, of which the glycosyl acceptor was the 5-(methoxycarbonyl)pentyl glycoside, were coupled using the trichloroacetimidate technology. The 12 azido functions present in the dodecasaccharide thus formed were then converted to amino groups using hydrogen sulfide as a reducing reagen...

journal_title：Glycoconjugate journal

authors： Ogawa Y,Kovac P

更新日期：1997-06-01 00:00:00

abstract：:Macrophages from various origins are known to express membrane lectins that mediate the endocytosis of mannose-bearing glycoconjugates. Most macrophage tumor cell-lines lack such receptors. In this paper we show by flow cytometry analysis that a newly generated macrophage hybridoma (2C11-12), which displays several ma...

journal_title：Glycoconjugate journal

authors： Pimpaneau V,Midoux P,Durand G,De Baetselier P,Monsigny M,Roche AC

更新日期：1989-01-01 00:00:00

abstract：:The human glioma D-54MG cell line grown in vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3'-isoLM1, an alteration that was do...

journal_title：Glycoconjugate journal

authors： Fredman P,Wikstrand CJ,Månsson JE,Reifenberger G,Bigner SH,Rasheed A,Svennerholm L,Bigner DD

更新日期：1996-06-01 00:00:00