Isolation and characterization of the potential receptor for wheat germ agglutinin from human neutrophils.

abstract：:UDP-2-(2-ketopropyl)galactose (1) has been utilized as a valuable probe for profiling proteins modified by O-GlcNAc. In this work, we developed a protocol for efficient synthesis of 1. Thus, 2-methallylgalactose derivative 11, a synthetic intermediate for the compound 1, was prepared by stereoselective iodination and ...

journal_title：Glycoconjugate journal

authors： Sakamoto Y,Ohta T,Ito Y

更新日期：2015-10-01 00:00:00

abstract：:UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II (GnT II; EC 2.4.1.143) is a medial-Golgi resident enzyme that catalyses an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides. Screening a cDNA library from Xenopus laevis ovary with a human ...

journal_title：Glycoconjugate journal

authors： Mucha J,Svoboda B,Kappel S,Strasser R,Bencur P,Fröhwein U,Schachter H,Mach L,Glössl J

更新日期：2002-03-01 00:00:00

abstract：:As an adjunct to existing thin layer and column chromatographic methods for the identification of glycolipids a method that utilizes the high pH anion chromatographic (HPAEC) analysis of the oligosaccharides released from the glycolipids by endoglycoceramidase has been developed. Using a Dionex Carbo Pak PA1 column an...

journal_title：Glycoconjugate journal

authors： Ruan S,Lloyd KO

更新日期：1994-06-01 00:00:00

abstract：:Three-dimensional structures of the natural substrate unit for the enzyme N-acetylglucosamine-transferase II, GIcNAc-Man3-GlcNAc2, were investigated by molecular modelling methods. Molecular dynamics (MD) and molecular mechanics calculations on two hexasaccharides, namely GlcNAc-Man3-GlcNAc2-Asn and GlcNAc-Man3-GlcNAc...

journal_title：Glycoconjugate journal

authors： Kozar T,Tvaroska I,Carver JP

更新日期：1998-02-01 00:00:00

abstract：:Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW approximately 32 kDa ...

journal_title：Glycoconjugate journal

authors： Raghav SK,Gupta B,Agrawal C,Saroha A,Das RH,Chaturvedi VP,Das HR

更新日期：2006-05-01 00:00:00

abstract：:A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose, N-acetyl glucosamine and N-acetyl neuraminic ...

journal_title：Glycoconjugate journal

authors： Aparna HS,Salimath PV

更新日期：1996-02-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids. The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients wi...

journal_title：Glycoconjugate journal

authors： Sturiale L,Bianca S,Garozzo D,Terracciano A,Agolini E,Messina A,Palmigiano A,Esposito F,Barone C,Novelli A,Fiumara A,Jaeken J,Barone R

更新日期：2019-12-01 00:00:00

abstract：:Nucleic acids bearing glycans of various structures have been under vigorous investigation in the past decade. The carbohydrate moieties of such complexes can serve as recognition sites for carbohydrate-binding proteins-lectins-and initiate receptor-mediated endocytosis. Therefore, carbohydrates can enhance cell targe...

journal_title：Glycoconjugate journal

authors： Yan H,Tram K

更新日期：2007-04-01 00:00:00

abstract：:In this study the comparative TLC immunostaining investigation of neutral GSLs and gangliosides from human skeletal and heart muscle is described. A panel of specific polyclonal and monoclonal antibodies as well as the GM1-specific choleragenoid were used for the overlay assays, combined with preceding neuraminidase t...

journal_title：Glycoconjugate journal

authors： Müthing J,Cacić M

更新日期：1997-01-01 00:00:00

abstract：:CLEC-2 was first identified by sequence similarity to C-type lectin-like molecules with immune functions and has been reported as a receptor for the platelet-aggregating snake venom toxin rhodocytin and the endogenous sialoglycoprotein podoplanin. Recent researches indicate that CLEC-2-deficient mice were lethal at th...

journal_title：Glycoconjugate journal

authors： Wang L,Ren S,Zhu H,Zhang D,Hao Y,Ruan Y,Zhou L,Lee C,Qiu L,Yun X,Xie J

更新日期：2012-08-01 00:00:00

abstract：:Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology. Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface. The major stru...

journal_title：Glycoconjugate journal

authors： Sharma S,Ding Y,Jarrell KF,Brockhausen I

更新日期：2018-12-01 00:00:00

abstract：:Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20-40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but research is in progress to...

journal_title：Glycoconjugate journal

authors： Maras M,van Die I,Contreras R,van den Hondel CA

更新日期：1999-02-01 00:00:00

abstract：:This review discusses the challenges involved in the characterization of the glycosylation of therapeutic glycoproteins. The focus is on methods that are most commonly used in regulatory filings and lot release testing of therapeutic glycoproteins. The different types of assays for carbohydrate analysis are reviewed, ...

journal_title：Glycoconjugate journal

authors： Higgins E

更新日期：2010-02-01 00:00:00

abstract：:Twenty different human and animal tissues were investigated for the presence of polyglycosylceramides. The glycolipids were isolated by peracetylation of dry tissue residues left after conventional lipid extraction, followed by extraction with chloroform and subsequent Sephadex LH-20, Sephadex LH-60 and silica gel chr...

journal_title：Glycoconjugate journal

authors： Miller-Podraza H,Stenhagen G,Larsson T,Andersson C,Karlsson KA

更新日期：1997-02-01 00:00:00

abstract：:The sugar chain-binding specificity of tomato lectin (LEA) against glycoproteins was investigated qualitatively using lectin blot analysis. Glycoproteins containing tri- and tetra-antennary complex-type N-glycans were stained with LEA. Unexpectedly, glycoproteins containing high mannose-type N-glycans and a horseradis...

journal_title：Glycoconjugate journal

authors： Oguri S

更新日期：2005-11-01 00:00:00

abstract：:A major impediment in the analysis of glycosaminoglycans is the difficulty to cleave quantitatively the glycosidic bonds because of the stabilisation of glycosidic bonds and of the relative instability of the liberated constituents. This manuscript describes a modified procedure of methanolysis in the presence of bari...

journal_title：Glycoconjugate journal

authors： Zanetta JP,Timmerman P,Leroy Y

更新日期：1999-10-01 00:00:00

abstract：:Using printed glycan array (PGA) we compared the results of antibody profiling in undiluted, moderately (1:15) and highly (1:100) diluted human blood serum. Undiluted serum is suitable for studying blood as a tissue in its native state, whereas to study the serum of newborns or small animals one usually has to dilute ...

journal_title：Glycoconjugate journal

authors： Shilova N,Navakouski M,Khasbiullina N,Blixt O,Bovin N

更新日期：2012-04-01 00:00:00

abstract：:Blood group antigen-related oligosaccharides have been implicated in growth regulation, cell mobility control and adhesion; we are therefore interested in the localization of receptors for these oligosaccharides in tumour cells. Labelled neoglycoconjugates that carry synthetic sugar structures are suitable tools to de...

journal_title：Glycoconjugate journal

authors： Kayser K,Bovin NV,Zemlyanukhina TV,Donaldo-Jacinto S,Koopmann J,Gabius HJ

更新日期：1994-08-01 00:00:00

abstract：:In this study we report on the first mass spectrometric (MS) investigation of gangliosides and preliminary assessment of the expression and structure in normal fetal neocortex in early developmental stages: 14th (Neo14) and 16th (Neo16) gestational weeks. Ganglioside analysis was carried out using a hybrid quadrupole ...

journal_title：Glycoconjugate journal

authors： Ghiulai RM,Sarbu M,Vukelić Ž,Ilie C,Zamfir AD

更新日期：2014-04-01 00:00:00

abstract：:Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens o...

journal_title：Glycoconjugate journal

authors： Nakamura Y,Miyata Y,Matsuo T,Shida Y,Hakariya T,Ohba K,Taima T,Ito A,Suda T,Hakomori SI,Saito S,Sakai H

更新日期：2019-10-01 00:00:00

abstract：:The human glioma D-54MG cell line grown in vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3'-isoLM1, an alteration that was do...

journal_title：Glycoconjugate journal

authors： Fredman P,Wikstrand CJ,Månsson JE,Reifenberger G,Bigner SH,Rasheed A,Svennerholm L,Bigner DD

更新日期：1996-06-01 00:00:00

abstract：:Mannose 6-phosphate receptor proteins (MPR 300 and 46) are type 1 transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. In a recent study we have purified both receptors from goat liver and raised antibodies. An ELISA method was developed that allowed quantification of both receptors in...

journal_title：Glycoconjugate journal

authors： Suresh K,Vegiraju SR,Nadimpalli SK

更新日期：2004-01-01 00:00:00

abstract：:Cell surface lipopolysaccharide (LPS) is a well characterized virulence determinant for the human pathogen Haemophilus influenzae, so an investigation of LPS in the less pathogenic Haemophilus parainfluenzae could yield important insights. Using a panel of 18 commensal H. parainfluenzae isolates we demonstrate that th...

journal_title：Glycoconjugate journal

authors： Young RE,Hood DW

更新日期：2013-08-01 00:00:00

abstract：:Human β-1,4-galactosyltransferase (β-1,4-GalT) V was shown to be involved in the biosynthesis of N-glycans, O-glycans and lactosylceramide (Lac-Cer) by in vitro studies. To determine its substrate specificity, enzymatic activity and its products were analyzed using mouse embryonic fibroblast (MEF) cells from β-1,4-Gal...

journal_title：Glycoconjugate journal

authors： Kumagai T,Sato T,Natsuka S,Kobayashi Y,Zhou D,Shinkai T,Hayakawa S,Furukawa K

更新日期：2010-10-01 00:00:00

abstract：:Sialosyl-Le(x) (SLex) and its positional isomer sialosyl-Le(a) are the epitopes recognized by the lectin domain of E- and P-selectins. Expression of SLex in polymorphonuclear leukocytes (PMN) plays an important role in recruitment of these cells at sites of inflammation through activation of selectins. We studied expr...

journal_title：Glycoconjugate journal

authors： Ito K,Handa K,Hakomori S

更新日期：1994-06-01 00:00:00

abstract：:The Maillard reaction was discovered in 1912 by Louis C. Maillard when he observed the browning phenomena with aroma formation in a heated solution containing a sugar and an amino acid. The Maillard reaction starts from the reactions between carbonyl groups of various sugars and amino groups of amino acids/ proteins, ...

journal_title：Glycoconjugate journal

authors： Murata M

更新日期：2020-09-10 00:00:00

abstract：:Chondroitin sulfate (CS) and dermatan sulfate (DS) interact with various extracellular molecules such as growth factors, cytokines/chemokines, neurotrophic factors, morphogens, and viral proteins, thereby playing roles in a variety of biological processes including cell adhesion, proliferation, tissue morphogenesis, n...

journal_title：Glycoconjugate journal

authors： Mizumoto S,Fongmoon D,Sugahara K

更新日期：2013-08-01 00:00:00

abstract：:Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presen...

journal_title：Glycoconjugate journal

authors： Du J,Liu S,Liang Q,Lin J,Jiang L,Chen F,Wei Z

更新日期：2019-06-01 00:00:00

abstract：:Recently, we demonstrated that the human xylosyltransferase II (XT-II) has enzymatic activity and is able to catalyze the initial and rate-limiting step in the biosynthesis of glycosaminoglycans (GAGs) like chondroitin and dermatan sulfate, as well as heparan sulfate and heparin. Therefore, this enzyme also very likel...

journal_title：Glycoconjugate journal

authors： Müller B,Prante C,Knabbe C,Kleesiek K,Götting C

更新日期：2013-04-01 00:00:00

abstract：:Proteoglycans contain a unique carbohydrate component, glycosaminoglycan, which consists of repeating, typically sulfated disaccharides, and is capable of interacting with diverse molecules. Specific, clustered arrangements of sulfate on the glycosaminoglycan backbone form binding sites for many biologically important...

journal_title：Glycoconjugate journal

authors： Yanagishita M,Podyma-Inoue KA,Yokoyama M

更新日期：2009-11-01 00:00:00