Analysis of sugar chain-binding specificity of tomato lectin using lectin blot: recognition of high mannose-type N-glycans produced by plants and yeast.


:The sugar chain-binding specificity of tomato lectin (LEA) against glycoproteins was investigated qualitatively using lectin blot analysis. Glycoproteins containing tri- and tetra-antennary complex-type N-glycans were stained with LEA. Unexpectedly, glycoproteins containing high mannose-type N-glycans and a horseradish peroxidase were stained with LEA. LEA blot analysis of the glycoproteins accompanied by treatment with exoglycosidase revealed that the binding site of LEA for the complex-type N-glycans was the N-acetyllactosaminyl side chains, whereas the proximal chitobiose core appeared to be the binding site of LEA for high mannose-type N-glycans. Despite these results, the glycoproteins did not inhibit the hemagglutinating activity of LEA. Among the chitin-binding lectins compared, potato tuber lectin showed specificity similar to LEA on lectin blot analysis, while Datura stramonium lectin and wheat germ agglutinin (WGA) did not interact with glycoproteins containing high mannose-type N-glycans, except that RNase B was stained by WGA. Based on these observations, LEA blot analysis was applied to sugar chain analysis of tomato glycoproteins. The most abundant LEA-reactive glycoprotein was purified from the exocarp of ripe tomato fruits, and was identified as the tomato anionic peroxidase1 (TAP1). These results suggest that LEA interacts with glycoproteins produced by tomatoes, which participate in biological activities in tomato plants.


Glycoconj J


Glycoconjugate journal


Oguri S




Has Abstract


2005-11-01 00:00:00












  • Structural analysis of N-glycans from allergenic grass, ragweed and tree pollens: core alpha1,3-linked fucose and xylose present in all pollens examined.

    abstract::The N-glycans from soluble extracts of ten pollens were examined. The pyridylaminated oligosaccharides derived from these sources were subject to gel filtration and reverse-phase HPLC, in conjunction with exoglycosidase digests, and in some cases matrix-assisted laser desorption-ionisation mass spectrometry. In compar...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Wilson IB,Altmann F

    更新日期:1998-11-01 00:00:00

  • Immunochemical characterization of synthetic hexa-, octa- and decasaccharide conjugate vaccines for Vibrio cholerae O:1 serotype Ogawa with emphasis on antigenic density and chain length.

    abstract::Cholera remains to be a global health problem without suitable vaccines for endemic control or outbreak relief. Here we describe a new parenteral vaccine based on neoglyco-conjugate of synthetic fragments of O-specific polysaccharide (O-SP) of Vibrio cholerae O1, serotype Ogawa. Hexa-, octa- and decasaccharides of the...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Ftacek P,Nelson V,Szu SC

    更新日期:2013-12-01 00:00:00

  • High level of sialate-O-acetyltransferase activity in lymphoblasts of childhood acute lymphoblastic leukaemia (ALL): enzyme characterization and correlation with disease status.

    abstract::Previous studies had established an over-expression of 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of childhood acute lymphoblastic leukaemia (ALL). Here, we report the discovery and characterization of sialate-O-acetyltransferase enzyme in ALL-cell lines and lymphoblasts from bone marrow of ch...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Mandal C,Srinivasan GV,Chowdhury S,Chandra S,Mandal C,Schauer R,Mandal C

    更新日期:2009-01-01 00:00:00

  • Polysaccharide-rich fraction of Termitomyces eurhizus accelerate healing of indomethacin induced gastric ulcer in mice.

    abstract::The current study aims to determine the healing activity of water soluble polysaccharide-rich fraction of a wild mushroom, Termitomyces eurhizus (TEps) against the indomethacin induced gastric ulceration in mice model. Gastric tissue histology, myeloperoxidase (MPO) activity, cyclooxygenases (COX) 1 and 2 expression, ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Chatterjee A,Khatua S,Chatterjee S,Mukherjee S,Mukherjee A,Paloi S,Acharya K,Bandyopadhyay SK

    更新日期:2013-11-01 00:00:00

  • A shift from N-glycolyl- to N-acetyl-sialic acid in the GM3 ganglioside impairs tumor development in mouse lymphocytic leukemia cells.

    abstract::Humans, in contrast to other mammals, do not synthesize N-glycolyl-neuraminic acid (Neu5Gc) due to a deletion in the gene (cmah) encoding the enzyme responsible for this conversion, the cytidine monophospho-N-acetyl-neuraminic acid hydroxylase (CMP-Neu5Ac hydroxylase). The detection of considerable amounts of Neu5Gc-s...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Casadesús AV,Fernández-Marrero Y,Clavell M,Gómez JA,Hernández T,Moreno E,López-Requena A

    更新日期:2013-10-01 00:00:00

  • Studies on the conformational behaviour of GlcNAc-Man3-GlcNAc2 oligosaccharides using molecular dynamics simulations.

    abstract::Three-dimensional structures of the natural substrate unit for the enzyme N-acetylglucosamine-transferase II, GIcNAc-Man3-GlcNAc2, were investigated by molecular modelling methods. Molecular dynamics (MD) and molecular mechanics calculations on two hexasaccharides, namely GlcNAc-Man3-GlcNAc2-Asn and GlcNAc-Man3-GlcNAc...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Kozar T,Tvaroska I,Carver JP

    更新日期:1998-02-01 00:00:00

  • Cloning and characterization of a sialidase from the filamentous fungus, Aspergillus fumigatus.

    abstract::A gene encoding a putative sialidase was identified in the genome of the opportunistic fungal pathogen, Aspergillus fumigatus. Computational analysis showed that this protein has Asp box and FRIP domains, it was predicted to have an extracellular localization, and a mass of 42 kDa, all of which are characteristics of ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Warwas ML,Yeung JH,Indurugalla D,Mooers AO,Bennet AJ,Moore MM

    更新日期:2010-07-01 00:00:00

  • Structural details and composition of Trichomonas vaginalis lipophosphoglycan in relevance to the epithelial immune function.

    abstract::Trichomonas vaginalis causes the most common non-viral sexually transmitted infection linked to increased risk of premature birth, cervical cancer and HIV. This study defines molecular domains of the parasite surface glycoconjugate lipophosphoglycan (LPG) with distinct functions in the host immunoinflammatory response...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Singh BN,Hayes GR,Lucas JJ,Sommer U,Viseux N,Mirgorodskaya E,Trifonova RT,Sassi RR,Costello CE,Fichorova RN

    更新日期:2009-01-01 00:00:00

  • Glycation changes the charge distribution of type I collagen fibrils.

    abstract::In aging and diabetes, glycation of collagen molecules leads to the formation of cross-links that could alter the surface charge on collagen fibrils, and hence affect the properties and correct functioning of a number of tissues. The electron-optical stain phosphotungstic acid (PTA) binds to positively charged amino a...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Hadley JC,Meek KM,Malik NS

    更新日期:1998-08-01 00:00:00

  • Enzymic synthesis of the trisaccharide core region of the carbohydrate chain of N-glycoprotein.

    abstract::Transmannosylation from mannotriose (Man beta 1-4Man beta 1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue of N,N'-diacetylchitobiose was regioselectively induced through the use of beta-D-mannanase from Aspergillus niger. The enzyme formed the trisaccharide Man beta 1-4GlcNAc beta 1-4GlcN...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Usui T,Suzuki M,Sato T,Kawagishi H,Adachi K,Sano H

    更新日期:1994-04-01 00:00:00

  • Carbohydrate-mediated cell adhesion involved in hematogenous metastasis of cancer.

    abstract::The carbohydrate determinants, sialyl Lewis A and sialyl Lewis X, which are frequently expressed on human cancer cells, serve as ligands for a cell adhesion molecule of the selectin family, E-selectin, which is expressed on vascular endothelial cells. These carbohydrate determinants are involved in the adhesion of can...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Kannagi R

    更新日期:1997-08-01 00:00:00

  • Alpha 1,4galactosyltransferase activity and Gb3Cer expression in human leukaemia/lymphoma cell lines.

    abstract::We have used two methods to evaluate the level of expression of Gb3Cer in several human leukaemia/lymphoma cell lines representative of the myeloid (K562, KG-1, HL-60, and lymphoid (Reh, Daudi, Raji, RPMI 8226, CCRF-CEM, MOLT-4) lineages blocked at varied stages of differentiation. TLC immunostaining of glycolipid ext...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Stults CL,Larsen RD,Macher BA

    更新日期:1995-10-01 00:00:00

  • Preparation of N-acetylneuraminic acid from delipidated egg yolk.

    abstract::Egg yolk, a large proportion of the egg, was studied for the preparation of N-acetylneuraminic acid (Neu5Ac). The delipidated hen egg yolk (DEY; 500 kg containing 0.2% w/w, Neu5Ac) was hydrolysed with HCl (pH 1.4) at 80 degrees C and neutralized with NaOH (pH 6.0). The mixture was filtered and electrodialysed until th...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Koketsu M,Juneja LR,Kawanami H,Kim M,Yamamoto T

    更新日期:1992-04-01 00:00:00

  • MUC1 gene polymorphism and gastric cancer--an epidemiological study.

    abstract::Gastric carcinoma is a major cause of cancer death worldwide and, like most human cancers, probably develops after environmental insults acting on normal individuals and/or individuals with increased genetic susceptibility. Mucins are attractive molecules to study the relationship between genetics and environment beca...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Carvalho F,Seruca R,David L,Amorim A,Seixas M,Bennett E,Clausen H,Sobrinho-Simões M

    更新日期:1997-01-01 00:00:00

  • A comparative study of lectin binding to cultured chick sternal chondrocytes and intact chick sternum.

    abstract::Cultured chondrocytes derived from the caudal and cephalic ends of embryonic chick sterna have been compared with each other and with whole sternum, by using a panel of 21 lectins to probe the distribution of oligosaccharides in glycoconjugates of cells and matrix at various times of culture or development. On culture...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: McClure SF,Stoddart RW,McClure J

    更新日期:1997-04-01 00:00:00

  • Expression analysis of a type S2 EUL-related lectin from rice in Pichia pastoris.

    abstract::Rice (Oryza sativa) expresses different putative carbohydrate-binding proteins belonging to the class of lectins containing an Euonymus lectin (EUL)-related domain, one of them being OrysaEULS2. The OrysaEULS2 sequence consists of a 56 amino acid N-terminal domain followed by the EUL sequence. In this paper the origin...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Al Atalah B,Rougé P,Smith DF,Proost P,Lasanajak Y,Van Damme EJ

    更新日期:2012-10-01 00:00:00

  • Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells.

    abstract::Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Axelsson MA,Hansson EM,Sikut R,Hansson GC

    更新日期:1998-08-01 00:00:00

  • Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases.

    abstract::Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-alpha-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1 and RGXT2 in Pi...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Petersen BL,Egelund J,Damager I,Faber K,Jensen JK,Yang Z,Bennett EP,Scheller HV,Ulvskov P

    更新日期:2009-12-01 00:00:00

  • Synthesis and X-ray crystallographic investigation of N-(3-deoxy-3-acetamido-β-D-glycopyranosyl)alkanamides as analogs of N-glycoprotein linkage region.

    abstract::As part of our ongoing program aimed at understanding the structural significance of GlcNAcβAsn linkage conserved in all eukaryotic N-glycoproteins, the present study reports on the synthesis and X-ray crystal structures of N-(3-deoxy-3-acetamido-β-D-glycopyranosyl)acetamide (Glc3NAcβNHAc) and the corresponding propio...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Mathiselvam M,Varghese B,Loganathan D

    更新日期:2011-12-01 00:00:00

  • Biological roles of sulfoglycolipids and pathophysiology of their deficiency.

    abstract::Mammalian sulfoglycolipids are comprised of two major members, sulfatide (SO(3)-3Gal-ceramide) and seminolipid (SO(3)-3Gal-alkylacylglycerol). Sulfatide is abundant in the myelin sheath and seminolipid is expressed on the spermatogenic cells. Cerebroside sulfotransferase (CST)-deficient mice generated by gene targetin...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Honke K,Zhang Y,Cheng X,Kotani N,Taniguchi N

    更新日期:2004-01-01 00:00:00

  • Structural analysis of novel rhamnose-branched oligosaccharides from the glycophosphosphingolipids of Leptomonas samueli.

    abstract::Mild alkaline hydrolysis of the glycophosphosphingolipids of the protozoan Leptomonas samueli liberated several phosphoinositol-containing oligosaccharides (PI-oligosaccharides), which were purified by high performance anion exchange chromatography. The oligosaccharides in the resulting four fractions were characteriz...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Previato JO,Wait R,Jones C,Mendonça-Previato L

    更新日期:1994-02-01 00:00:00

  • Metabolic fate of milk glycosaminoglycans in breastfed and formula fed newborns.

    abstract::In this study, the content, structure and residual percentages of glycosaminoglycans (GAGs) in the feces of seven breastfed newborns after ingesting a known amount of milk were studied. A comparison was made with five newborns fed with formula milk. Characterization of GAGs from milk and feces samples was performed ac...

    journal_title:Glycoconjugate journal

    pub_type: 临床试验,杂志文章


    authors: Maccari F,Mantovani V,Gabrielli O,Carlucci A,Zampini L,Galeazzi T,Galeotti F,Coppa GV,Volpi N

    更新日期:2016-04-01 00:00:00

  • Glycosphingolipids in feces of germ-free rats as a source for studies of developmental changes of intestinal epithelial cell surface carbohydrates.

    abstract::Non-acid and acid glycosphingolipids were isolated from feces of one litter of germ-free rats from day 17 to day 51. Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384-89] were also found in the feces of these germ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Larson G,Midtvedt T

    更新日期:1989-01-01 00:00:00

  • Structure elucidation of NeuAc, NeuGc and Kdn-containing O-glycans released from Triturus alpestris oviductal mucins. Characterization of the poly LacdiNAc sequence: HSO3(4)(GalNAcbeta1-4GlcNAcbeta1-3)1-3GalNAcbeta1-4(GlcNAcbeta1-3)0-1GlcNAcbeta1-6GalNAc-

    abstract::Eggs from Amphibia are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of highly glycosylated mucin-type glycoproteins containing up to 60% of carbohydrates, which display a remarkable species-specificity. This material obtained from Triturus alpestris was submitt...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Florea D,Maes E,Guérardel Y,Page A,Zanetta JP,Cogalniceanu D,Strecker G

    更新日期:2006-07-01 00:00:00

  • Hypoxia remodels the composition of the constituent ceramide species of HexCer and Hex2Cer with phytosphingosine and hydroxy fatty acids in human colon cancer LS174T cells.

    abstract::Oxygen-requiring enzymes, such as Δ4-desaturase (dihydroceramide desaturase), sphingolipid Δ4-desaturase/C-4-hydroxylase, and fatty acid 2-hydroxylase are involved in ceramide synthesis. We prepared free ceramides, sphingomyelins and glycosphingolipids (GSLs) from cancer cells cultivated under conditions of normoxia a...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Tanaka K,Tamiya-Koizumi K,Yamada M,Murate T,Kannagi R,Kyogashima M

    更新日期:2015-11-01 00:00:00

  • A full assignment of proton resonances for an alpha(1-3)-linked fucose residue in keratan sulphate from bovine articular cartilage.

    abstract::Full proton NMR assignments have been achieved for the alpha(1-3)-linked fucose residues contained in alkaline borohydride reduced keratan sulphate chains derived from bovine articular cartilage. This involved 500 MHz spectroscopy at 60 degrees C and included COSY and RELAYED-COSY determinations. ...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Huckerby TN,Nieduszynski IA,Brown GM,Cockin GH

    更新日期:1991-02-01 00:00:00

  • Structural analysis of water-soluble fractions obtained from Aspergillus fumigatus mycelium.

    abstract::Fractions were prepared from the water-soluble components of Aspergillus fumigatus mycelium either by lectin-affinity chromatography or salt precipitation. While they varied considerably in their amino-acid composition, each contained a preponderance of aspartic and glutamic acids. 13C-NMR spectroscopy of these fracti...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Hearn VM,Griffiths BL,Gorin PA

    更新日期:1989-01-01 00:00:00

  • Epigenetic regulation of ganglioside expression in neural stem cells and neuronal cells.

    abstract::The structural diversity and localization of cell surface glycosphingolipids (GSLs), including gangliosides, in glycolipid-enriched microdomains (GEMs, also known as lipid rafts) render them ideally suited to play important roles in mediating intercellular recognition, interactions, adhesion, receptor function, and si...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章,评审


    authors: Itokazu Y,Tsai YT,Yu RK

    更新日期:2017-12-01 00:00:00

  • Glycosylation of the N-terminal potential N-glycosylation sites in the human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI).

    abstract::Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and hFucTVI: Asn46 and Asn91...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Christensen LL,Bross P,Ørntoft TF

    更新日期:2000-12-01 00:00:00

  • Identification, expression and tissue distribution of cytidine 5'-monophosphate N-acetylneuraminic acid synthetase activity in the rat.

    abstract::We report the postnatal developmental profiles of N-acetylneuraminic acid cytidylyltransferase (EC (CMP-Neu5Ac synthetase) in different rat tissues. This enzyme, which catalyses the activation of NeuAc to CMP-Neu5Ac, was detected in brain, kidney, heart, spleen, liver, stomach, intestine, lung, thymus, prost...

    journal_title:Glycoconjugate journal

    pub_type: 杂志文章


    authors: Revilla-Nuin B,Reglero A,Feo JC,Rodriguez-Aparicio LB,Ferrero MA

    更新日期:1998-03-01 00:00:00