Glycosylation potential of human prostate cancer cell lines.

abstract：:This review discusses the challenges involved in the characterization of the glycosylation of therapeutic glycoproteins. The focus is on methods that are most commonly used in regulatory filings and lot release testing of therapeutic glycoproteins. The different types of assays for carbohydrate analysis are reviewed, ...

journal_title：Glycoconjugate journal

authors： Higgins E

更新日期：2010-02-01 00:00:00

abstract：:In this study the comparative TLC immunostaining investigation of neutral GSLs and gangliosides from human skeletal and heart muscle is described. A panel of specific polyclonal and monoclonal antibodies as well as the GM1-specific choleragenoid were used for the overlay assays, combined with preceding neuraminidase t...

journal_title：Glycoconjugate journal

authors： Müthing J,Cacić M

更新日期：1997-01-01 00:00:00

abstract：:The IgG Fc glycans strongly influence the Fcγ receptor interactions and Fc-mediated effector mechanisms. Changes in the structure of IgG glycans are associated with various diseases, such as infections and autoimmunity. However, the possible role of Fc glycans in tumor immunity is not yet fully understood. The aim of ...

journal_title：Glycoconjugate journal

authors： Kodar K,Stadlmann J,Klaamas K,Sergeyev B,Kurtenkov O

更新日期：2012-01-01 00:00:00

abstract：:Three-dimensional structures of the natural substrate unit for the enzyme N-acetylglucosamine-transferase II, GIcNAc-Man3-GlcNAc2, were investigated by molecular modelling methods. Molecular dynamics (MD) and molecular mechanics calculations on two hexasaccharides, namely GlcNAc-Man3-GlcNAc2-Asn and GlcNAc-Man3-GlcNAc...

journal_title：Glycoconjugate journal

authors： Kozar T,Tvaroska I,Carver JP

更新日期：1998-02-01 00:00:00

abstract：:We report the postnatal developmental profiles of N-acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CMP-Neu5Ac synthetase) in different rat tissues. This enzyme, which catalyses the activation of NeuAc to CMP-Neu5Ac, was detected in brain, kidney, heart, spleen, liver, stomach, intestine, lung, thymus, prost...

journal_title：Glycoconjugate journal

authors： Revilla-Nuin B,Reglero A,Feo JC,Rodriguez-Aparicio LB,Ferrero MA

更新日期：1998-03-01 00:00:00

abstract：:Trichomonas vaginalis causes the most common non-viral sexually transmitted infection linked to increased risk of premature birth, cervical cancer and HIV. This study defines molecular domains of the parasite surface glycoconjugate lipophosphoglycan (LPG) with distinct functions in the host immunoinflammatory response...

journal_title：Glycoconjugate journal

authors： Singh BN,Hayes GR,Lucas JJ,Sommer U,Viseux N,Mirgorodskaya E,Trifonova RT,Sassi RR,Costello CE,Fichorova RN

更新日期：2009-01-01 00:00:00

abstract：:Disialosyl globopentaosylceramide (DSGb5) is a ganglioside originally isolated from tissue extracts of renal cell carcinoma (RCC) with metastasis. Previous in vitro experiments have suggested that DSGb5 promotes metastasis by enhancing the migration of RCC cells and downregulating NK cell cytotoxicity against RCC cell...

journal_title：Glycoconjugate journal

authors： Itoh J,Ito A,Shimada S,Kawasaki Y,Kakoi N,Saito H,Mitsuzuka K,Watanabe M,Satoh M,Saito S,Arai Y

更新日期：2017-04-01 00:00:00

abstract：:The lectins Sambucus nigra agglutinin (SNA) and Ricinus communis agglutinin (RCA), specific for alpha2,6 linked sialylation, and terminal galactose respectively were used to study the occurrence, linkage and distribution of human immunoglobulin G (IgG) sialylation. SNA was shown to bind N-glycan alpha2,6-linked sialic...

journal_title：Glycoconjugate journal

authors： Dalziel M,McFarlane I,Axford JS

更新日期：1999-12-01 00:00:00

abstract：:By employing a bovine UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyl transferase (O-GalNAc transferase) cDNA as a probe, we isolated four overlapping cDNAs from a porcine lung cDNA library. Both the nucleotide sequence of the porcine cDNA and the predicted primary structure of the protein (559 amino aci...

journal_title：Glycoconjugate journal

authors： Yoshida A,Hara T,Ikenaga H,Takeuchi M

更新日期：1995-12-01 00:00:00

abstract：:Colominic acid is an alpha2,8-linked sialic acid polymer produced by Escherichia coli. We found that synthetic sulfated-colominic acids (SC) remarkably inhibited the cytotoxicity of bee and snake venom toward mouse fibroblast cells, but colominic acids showed no inhibition themselves, indicating the important role of ...

journal_title：Glycoconjugate journal

authors： Oda Y,Kinoshita M,Hamada K,Nakayama K,Ohta Y,Yamaguchi S,Tsukada Y,Kawai Y,Kakehi K

更新日期：1999-08-01 00:00:00

abstract：:A water-soluble polysaccharide CSPS-2B-2 with a molecular mass of 8.8 kDa, was obtained from the fruits of Capparis spinosa L. Chemical and NMR spectral analysis verified CSPS-2B-2 was a linear poly-(1-4)-α-D-galactopyranosyluronic acid in which 12.9±0.4% of carboxyl groups existed as methyl ester and 2.6±0.1% of D-Ga...

journal_title：Glycoconjugate journal

authors： Wang H,Wang H,Shi S,Duan J,Wang S

更新日期：2012-08-01 00:00:00

abstract：:The interaction between Urtica dioica agglutinin (UDA) and N-acetylglucosamine (GlcNAc) and its beta(1-4)-linked oligomers was studied by fluorescence titration and isothermal titration microcalorimetry. UDA possesses one significant binding site that can be measured calorimetrically. This site is composed of three su...

journal_title：Glycoconjugate journal

authors： Lee RT,Gabius HJ,Lee YC

更新日期：1998-07-01 00:00:00

abstract：:The partitioning of the overall molecular charge distribution into atom centered monopole charges, while quantum mechanically ill-defined, is nevertheless a technique which finds applications in several broad classes of chemical problems. Charges derived from fits to electrostatic potentials have an intuitive appeal s...

journal_title：Glycoconjugate journal

authors： Carey C,Chirlian LE,Francl MM,Gange DM

更新日期：1997-06-01 00:00:00

abstract：:In this report we present the enzymatic characterization of CMP-NeuAc:Gal beta 1-4GlcNAc-R alpha(2-3)-sialyltransferase from human placenta using placenta membranes as an enzyme preparation. This sialyltransferase is highly sensitive to detergents and prefers type 2 chain (Gal beta 1-4GlcNAc) over type 1 chain (Gal be...

journal_title：Glycoconjugate journal

authors： Nemansky M,van den Eijnden DH

更新日期：1993-02-01 00:00:00

abstract：:Potato lectin (Solanum tuberosum agglutinin, STA) is an unusual glycoprotein containing approximately 50% carbohydrates by weight. Of the total carbohydrates, 92% is contributed by L: -arabinose, which are O-linked to hydroxyproline residues. The ferric chloride-orcinol assay (Bial's test), which is specific for pento...

journal_title：Glycoconjugate journal

authors： Pramod SN,Venkatesh YP

更新日期：2006-11-01 00:00:00

abstract：:Twenty different human and animal tissues were investigated for the presence of polyglycosylceramides. The glycolipids were isolated by peracetylation of dry tissue residues left after conventional lipid extraction, followed by extraction with chloroform and subsequent Sephadex LH-20, Sephadex LH-60 and silica gel chr...

journal_title：Glycoconjugate journal

authors： Miller-Podraza H,Stenhagen G,Larsson T,Andersson C,Karlsson KA

更新日期：1997-02-01 00:00:00

abstract：:Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, of...

journal_title：Glycoconjugate journal

authors： Lima MA,Cavalheiro RP,M Viana G,Meneghetti MCZ,Rudd TR,Skidmore MA,Powell AK,Yates EA

更新日期：2017-06-01 00:00:00

abstract：:Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. In this study, we used multiple lectin assays to analyze glycan patterns of serum GP73 and evaluated its diagnostic value for distinguishing hepatocellular carcinoma (HCC) from liver cirrhosis (LC). Firstly, ...

journal_title：Glycoconjugate journal

authors： Jiang K,Shang S,Li W,Guo K,Qin X,Zhang S,Liu Y

更新日期：2015-12-01 00:00:00

abstract：:To reveal the function of the carbohydrate portion of glycopeptides and glycoproteins, we chemo-enzymatically synthesized artificially N-glycosylated derivatives of eel calcitonin and studied their three-dimensional structure and biological activity. The CD and NMR spectra in trifluoroethanol-H(2)O solution showed tha...

journal_title：Glycoconjugate journal

authors： Tagashira M,Tanaka A,Hisatani K,Isogai Y,Hori M,Takamatsu S,Fujibayashi Y,Yamamoto K,Haneda K,Inazu T,Toma K

更新日期：2001-06-01 00:00:00

abstract：:Proteoglycans and glycosaminoglycans modulate numerous cellular processes relevant to tumour progression, including cell proliferation, cell-matrix interactions, cell motility and invasive growth. Among the glycosaminoglycans with a well-documented role in tumour progression are heparan sulphate, chondroitin/dermatan ...

journal_title：Glycoconjugate journal

authors： Viola M,Brüggemann K,Karousou E,Caon I,Caravà E,Vigetti D,Greve B,Stock C,De Luca G,Passi A,Götte M

更新日期：2017-06-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids. The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients wi...

journal_title：Glycoconjugate journal

authors： Sturiale L,Bianca S,Garozzo D,Terracciano A,Agolini E,Messina A,Palmigiano A,Esposito F,Barone C,Novelli A,Fiumara A,Jaeken J,Barone R

更新日期：2019-12-01 00:00:00

abstract：:Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describ...

journal_title：Glycoconjugate journal

authors： Axelsson MA,Hansson EM,Sikut R,Hansson GC

更新日期：1998-08-01 00:00:00

abstract：:Syndecan-4 is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family. Syndecan-4-deficient [(Synd4(-/-)] mice were produced to clarify the in vivo role of syndecan-4. Synd4(-/-) mice were more susceptible to kappa-carrageenan-induced nephropathy, and the placental labyrinth from the deficient em...

journal_title：Glycoconjugate journal

authors： Ishiguro K,Kojima T,Muramatsu T

更新日期：2002-05-01 00:00:00

abstract：:The hallmarks of cancer are characterized by functional capabilities that allow cancer cells to survive, proliferate and disseminate during the multistep tumorigenesis. Cancer being a cellular disease, changes in cellular glycoproteins play an important role in malignant transformation and cancer progression. The pres...

journal_title：Glycoconjugate journal

authors： Vajaria BN,Patel PS

更新日期：2017-04-01 00:00:00

abstract：:Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the ...

journal_title：Glycoconjugate journal

authors： Volpi N,Zampini L,Maccari F,Santoro L,Galeotti F,Galeazzi T,Gabrielli O,Coppa GV

更新日期：2013-10-01 00:00:00

abstract：:Neutrophils participate in host protection and central to this process is the regulation of oxidative mechanisms. We purified by affinity chromatography the receptor for the GlcNAc-specific WGA from CD14+ CD16+ cell lysates (WGAr). The receptor is a 141 kDa glycoprotein constituted by two subunits of 78 and 63 kDa. It...

journal_title：Glycoconjugate journal

authors： Solórzano C,Bouquelet S,Pereyra MA,Blanco-Favela F,Slomianny MC,Chavez R,Lascurain R,Zenteno E,Agundis C

更新日期：2006-11-01 00:00:00

abstract：:The modulation of GnT-V activity by signaling molecules in PI-3-K/PKB pathway in human hepatocarcinoma cell line 7721 was studied. GnT-V activity was determined after the transfection of sense or antisense cDNA of PKB into the cells, as well as the addition of activators, specific inhibitors, and the antibodies to the...

journal_title：Glycoconjugate journal

authors： Guo HB,Shen ZH,Huang CX,Ma J,Huang Y,Chen HL

更新日期：2000-05-01 00:00:00

abstract：:UDP-2-(2-ketopropyl)galactose (1) has been utilized as a valuable probe for profiling proteins modified by O-GlcNAc. In this work, we developed a protocol for efficient synthesis of 1. Thus, 2-methallylgalactose derivative 11, a synthetic intermediate for the compound 1, was prepared by stereoselective iodination and ...

journal_title：Glycoconjugate journal

authors： Sakamoto Y,Ohta T,Ito Y

更新日期：2015-10-01 00:00:00

abstract：:Sialosyl-Le(x) (SLex) and its positional isomer sialosyl-Le(a) are the epitopes recognized by the lectin domain of E- and P-selectins. Expression of SLex in polymorphonuclear leukocytes (PMN) plays an important role in recruitment of these cells at sites of inflammation through activation of selectins. We studied expr...

journal_title：Glycoconjugate journal

authors： Ito K,Handa K,Hakomori S

更新日期：1994-06-01 00:00:00

abstract：:Synthesis and antigenic reactivity of 6-O-sulfo sialylparagloboside (SPG) and sialyl Lewis X (sLeX) neo-glycolipids containing lactamized neuraminic acid are described. The suitably protected GlcNAc-beta (1-->3)-Gal-beta (1-->4)-GlcOSE derivative was glycosylated with NeuTFAc-alpha (2-->3)-Gal imidate to give NeuTFAc-...

journal_title：Glycoconjugate journal

authors： Yamaguchi M,Ishida H,Kanamori A,Kannagi R,Kiso M

更新日期：2005-03-01 00:00:00