Abstract：
BACKGROUND:Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring long-term evaluations. METHODS & RESULTS:Immune responses of Balb/c mice were assessed by specific and neutralizing antibodies, interferon-γ ELISpot assay, and cytotoxic T-lymphocyte cytotoxicity assay. HTNV-challenge assay identified long-term protection. Safety was confirmed by histological and behavioral analysis. Epitope-spreading phenomenon was noted, revealing two sets of dominant T-cell epitopes cross-species. CONCLUSION:pVAX-LAMP/Gn established memory responses within a long-term protection. Lysosome-targeted strategy showed promise on Gn-based DNA vaccine and further investigations are warranted in other immunogenic Hantaviral antigens.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Jiang DB,Sun LJ,Cheng LF,Zhang JP,Xiao SB,Sun YJ,Yang SY,Wang J,Zhang FL,Yang Kdoi
10.1016/j.antiviral.2016.12.001subject
Has Abstractpub_date
2017-02-01 00:00:00pages
32-39eissn
0166-3542issn
1872-9096pii
S0166-3542(16)30582-4journal_volume
138pub_type
杂志文章abstract：:An estimated 15-20 million individuals are co-infected by hepatitis B and hepatitis D virus worldwide and are at high risk of developing end-stage liver disease, including hepatocellular carcinoma. While HBV viremia can now be controlled in the vast majority of individuals by nucleoside analogs, leading to a delay of ...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2015.08.009
更新日期：2015-10-01 00:00:00
abstract：:Treatment of the chronic hepatitis C virus (HCV) infection is an unmet medical need, and the HCV NS3 serine protease (NS3-SP) has been used as an attractive target of antiviral screening against HCV. To find naturally chemical entities as lead compounds from which novel anti-HCV agents could be developed, bioassay-gui...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.06.001
更新日期：2007-10-01 00:00:00
abstract：:Desferrioxamine (DFO) is commonly used in therapy as a chelator of ferric ion in disorders of iron overload. We found that DFO inhibits human cytomegalovirus (HCMV) replication in infected cultures of human foreskin fibroblasts (HFF) at concentrations that have been achieved in humans with no significant adverse effec...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)90095-7
更新日期：1994-09-01 00:00:00
abstract：:Enterovirus D68 (EV-D68) is a member of the Picornavirus family and a causative agent of respiratory diseases in children. The incidence of EV-D68 infection has increased worldwide in recent years. Thus far, there are no approved antiviral agents or vaccines for EV-D68. Here, we show that methyl-β-cyclodextrin (MβCD),...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104752
更新日期：2020-04-01 00:00:00
abstract：:Vaccination against smallpox may result in a variety of complications, ranging in severity from benign to lethal. Universal vaccination was halted in the US in 1972, so almost half the present population has never been vaccinated. Because side effects occur most often in first-time vaccinees, current plans for rapid l...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(03)00008-1
更新日期：2003-04-01 00:00:00
abstract：:There are no drugs approved specifically to treat disseminated adenovirus (Ad) infections in humans. Cidofovir is active against Ad in cell culture, and it is used frequently in the clinic with disseminated infection in pediatric transplant patients; however, controlled clinical studies have not been conducted to prov...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.10.005
更新日期：2014-12-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2019.104613
更新日期：2019-11-01 00:00:00
abstract：:The effects of HIV-1 protease inhibitors on proteolytic processing and infectivity of virions produced from lymphocytes chronically infected with the virus were studied. Protease inhibition was detected by the accumulation of the polyprotein precursors Pr55gag and Pr160gag-pol and their cleavage intermediates. Immunob...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(99)00074-1
更新日期：2000-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(88)90003-4
更新日期：1988-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104593
更新日期：2019-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.12.020
更新日期：2017-03-01 00:00:00
abstract：:The essential role of SMAR1 in HIV-1 transcription and LTR driven gene expression suggests SMAR1 as an HIV dependency factor (HDF) and a potential anti-HIV therapeutic target. Here, we report for the first time, anti-HIV activity of 8 novel isothiocyanate (ITC) derivatives that differentially stabilise SMAR1. Out of 8...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104648
更新日期：2020-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2010.08.004
更新日期：2010-10-01 00:00:00
abstract：:Nucleic acid polymers (NAPs) block the release of HBsAg from infected hepatocytes. These compounds have been previously shown to have the unique ability to eliminate serum surface antigen in DHBV-infected Pekin ducks and achieve multilog reduction of HBsAg or HBsAg loss in patients with chronic HBV infection and HBV/H...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.10.022
更新日期：2018-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2011.08.016
更新日期：2011-11-01 00:00:00
abstract：:Interferon alpha-2b (IFN) alone or in combination with Ribavirin is approved in the United States for the treatment of chronic hepatitis C virus (HCV) infection. We have previously reported that the glucosidase inhibitor, n-butyl deoxynojirimycin (nB-DNJ) inhibits the production of infectious bovine diarrhea virus (BV...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(02)00075-x
更新日期：2002-09-01 00:00:00
abstract：:Yellow fever virus (YFV), a member of the genus Flavivirus, is a mosquito-borne virus found in tropical regions of Africa and South America that causes severe hepatic disease and death in humans. Despite the availability of effective vaccines, YFV is responsible for an estimated 200,000 cases and 30,000 deaths annuall...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2012.02.001
更新日期：2012-04-01 00:00:00
abstract：:Venezuelan equine encephalitis virus (VEEV) may cause encephalitis in humans, for which no FDA-approved antiviral treatment is available. Carbocyclic cytosine (carbodine) has broad-spectrum activity but toxicity has limited its utility. It was anticipated that one of the enantiomers of carbodine would show enhanced ac...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2008.07.002
更新日期：2008-12-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2005.11.001
更新日期：2006-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(98)00015-1
更新日期：1998-06-01 00:00:00
abstract：:Influenza A virus has been responsible for widespread human epidemics because it is readily transmitted from humans to humans by aerosol. Recent events have highlighted the potential of influenza A virus as a bioterrorist weapon: the high virulence of the influenza A virus that infected people in Hong Kong in 1997; an...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(02)00207-3
更新日期：2003-01-01 00:00:00
abstract：:The heterocycle, 4,6-dibenzamidopyrazolo[3,4-d]pyrimidine (DBAPP), inhibited cytopathology induced by human, mouse, and vervet monkey cytomegaloviruses (CMV) in vitro at 0.2 to 0.5 microM, but did not inhibit cell replication at less than or equal to 30 microM. Herpes simplex viruses were unaffected by the inhibitor. ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(90)90040-e
更新日期：1990-01-01 00:00:00
abstract：:Natural killer (NK) cells are a crucial component of the host innate immune system. We investigated the noncytolytic anti-human immunodeficiency virus (HIV) activity of NK cells in chronically HIV-infected immune cells. Supernatants collected from NK cell cultures (both primary NK cells and NK cell lines, YTS and NK 9...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.08.006
更新日期：2007-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2005.05.001
更新日期：2005-08-01 00:00:00
abstract：:Cytomegalovirus (CMV) is the most common cause of congenital infection in the developed world and can lead to a life-threatening disease. We therefore developed an animal model to evaluate candidate anti-CMV drugs and to further define the pathogenesis of CMV infections. Newborn guinea pigs were infected by intraperit...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(03)00151-7
更新日期：2003-09-01 00:00:00
abstract：:Carbohydrate-binding proteins (CBP) can be isolated from a variety of species, including procaryotes (i.e. cyanobacteria), sea corals, algae, plants, invertebrates and vertebrates. A number of them, in particular those CBP that show specific recognition for mannose (Man) and N-acetylglucosamine (GlcNAc) are endowed wi...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2006.02.004
更新日期：2006-09-01 00:00:00
abstract：:Stilbenoids including resveratrol contain the basic structural unit of 1,2-diphenylethylene. Naturally occurring stilbenoids have broad structural features due to oligomerization and modifications and some have demonstrated potent biological activities. In an effort to identify bioactive stilbenoids, we screened a gro...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2012.05.001
更新日期：2012-07-01 00:00:00
abstract：:Serum from wild alligators was collected and tested for antibiotic activity against three enveloped viruses using cell-based assays. Alligator serum demonstrated antiviral activities against human immunodeficiency virus type 1 (HIV-1; IC50=0.9%), West Nile virus (WNV; IC50=4.3%), and Herpes simplex virus type 1 (HSV-1...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2004.12.007
更新日期：2005-04-01 00:00:00
abstract：:The ability to control plant viral diseases with chemicals has great potential value for agriculture, but few chemicals are available to date due to the difficulty in obtaining effective drugs. IMP dehydrogenase is an enzyme which catalyzes the conversion of inosine 5'-monophosphate to xanthosine 5'-monophosphate in t...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.10.007
更新日期：2007-03-01 00:00:00
abstract：:IRES-driven translation plays an essential role in picornavirus infection. However, there are seldom reports of compounds targeting this pathway with effective protection in animal models. Here, we identified emetine, an antiprotozoal drug, which inhibits EV-A71 with an EC50 value of 0.04 μM and a CC50 value of 10 μM ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104650
更新日期：2020-01-01 00:00:00
