当前位置： SCI文献检索 > ANTIVIRAL RESEARCH期刊下所有文献 > Antiviral activity of serum from the American alligator (Alligator mississippiensis).

Antiviral activity of serum from the American alligator (Alligator mississippiensis).

Abstract：

:Serum from wild alligators was collected and tested for antibiotic activity against three enveloped viruses using cell-based assays. Alligator serum demonstrated antiviral activities against human immunodeficiency virus type 1 (HIV-1; IC50=0.9%), West Nile virus (WNV; IC50=4.3%), and Herpes simplex virus type 1 (HSV-1; IC50=3.4%). The inhibitory concentration (IC50) is defined as the concentration of serum that inhibits 50% of viral activity. The antiviral effects of the alligator serum were difficult to evaluate at high concentrations due to the inherent toxicity to the mammalian cells used to assay viral activities. The TC50 (serum concentration that reduces cell viability to 50%) values for the serum in the HIV-1, WNV, and HSV-1 assays were 32.8, 36.3 and 39.1%, respectively. Heat-treated serum (56 degrees C, 30 min) displayed IC50 values of >50, 9.8 and 14.9% for HIV-1, WNV and HSV-1 viruses, respectively. In addition, the TC50 values using heat-treated serum were substantially elevated for all three assays, relative to untreated serum (47.3 to >50%). Alligator serum complement activity has been shown to be heat labile under these conditions. HIV-1 antiviral action was heat-sensitive, and thus possibly due to the action of serum complement, while the anti-WNV and anti-HSV-1 activities were not heat labile and thus probably not complement mediated.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Merchant ME,Pallansch M,Paulman RL,Wells JB,Nalca A,Ptak R

doi

10.1016/j.antiviral.2004.12.007

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

35-8

issue

1

eissn

0166-3542

issn

1872-9096

pii

S0166-3542(05)00009-4

journal_volume

66

pub_type

杂志文章

相关文献

ANTIVIRAL RESEARCH文献大全
  • Phenotypic and genotypic characterization of acyclovir-resistant clinical isolates of herpes simplex virus.

    abstract：:Sixteen herpes simplex virus type 1 (HSV-1) and four type 2 (HSV-2) isolates resistant to acyclovir (ACV) were characterized retrospectively for drug resistance. Phenotypic testing was performed by means of tetrazolium reduction assay and genotypic analysis was carried out by sequencing of thymidine kinase (TK) and DN...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.03.002

    authors： Sauerbrei A,Deinhardt S,Zell R,Wutzler P

    更新日期：2010-06-01 00:00:00

  • SeqHepB: a sequence analysis program and relational database system for chronic hepatitis B.

    abstract：:SeqHepB is a combination of a HBV genome sequence analysis program and a relational database that houses data collected from multiple data sources. Registered users can access the sequence analysis component of SeqHepB online for rapid and detailed interrogation of HBV genomic sequences. Its main function is to determ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2006.11.014

    authors： Yuen LK,Ayres A,Littlejohn M,Colledge D,Edgely A,Maskill WJ,Locarnini SA,Bartholomeusz A

    更新日期：2007-07-01 00:00:00

  • Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41.

    abstract：:Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents tha...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2008.05.006

    authors： Nishikawa H,Kodama E,Sakakibara A,Fukudome A,Izumi K,Oishi S,Fujii N,Matsuoka M

    更新日期：2008-10-01 00:00:00

  • Chikungunya virus pathogenesis: From bedside to bench.

    abstract：:Chikungunya virus (CHIKV) is an arbovirus transmitted to humans by mosquito bite. A decade ago, the virus caused a major outbreak in the islands of the Indian Ocean, then reached India and Southeast Asia. More recently, CHIKV has emerged in the Americas, first reaching the Caribbean and now extending to Central, South...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2015.07.002

    authors： Couderc T,Lecuit M

    更新日期：2015-09-01 00:00:00

  • Long-term inhibition of HIV-1 replication with RNA interference against cellular co-factors.

    abstract：:In this study we tested whether HIV-1 replication could be inhibited by stable RNAi-mediated knockdown of cellular co-factors. Cell lines capable of expressing shRNAs against 30 candidate co-factors implicated at different steps of the viral replication cycle were generated and analyzed for effects on cell viability a...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.11.005

    authors： Eekels JJ,Geerts D,Jeeninga RE,Berkhout B

    更新日期：2011-01-01 00:00:00

  • Adefovir dipivoxil efficiently inhibits the proliferation of pseudorabies virus in vitro and in vivo.

    abstract：:Since 2011, highly pathogenic pseudorabies virus (PRV) variants that emerged on many farms in China have posed major economic burdens to the animal industry and have even recently caused several human cases of viral encephalitis. Currently, there are no approved effective drugs to treat PRV associated diseases in huma...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2021.105014

    authors： Wang G,Chen R,Huang P,Hong J,Cao J,Wu Q,Zheng W,Lin L,Han Q,Chen Y,Xia N

    更新日期：2021-01-07 00:00:00

  • Targeting dengue virus NS4B protein for drug discovery.

    abstract：:The flavivirus nonstructural 4B protein (NS4B) has recently emerged as a valid antiviral target for drug discovery. Here we review (i) the current understanding of the structure and function of DENV NS4B, (ii) the approaches that have been taken to identify NS4B inhibitors, and (iii) the known inhibitors of flavivirus...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2015.03.007

    authors： Xie X,Zou J,Wang QY,Shi PY

    更新日期：2015-06-01 00:00:00

  • Dual effects of the novel ingenol derivatives on the acute and latent HIV-1 infections.

    abstract：:The latent reservoir of HIV-1 in resting memory CD4+ T cells serves as a major barrier to curing HIV-1 infection. Reactivation of latent HIV-1 is proposed as a promising strategy for the clearance of the viral reservoirs. Because of the limitations of current latency reversal agents (LRAs), identification of new LRAs ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2019.104555

    authors： Yang H,Li X,Yang X,Lu P,Wang Y,Jiang Z,Pan H,Zhao L,Zhu Y,Khan IU,Shen Y,Lu H,Zhang T,Jiang G,Ma Z,Wu H,Zhu H

    更新日期：2019-09-01 00:00:00

  • Anti-HIV (human immunodeficiency virus) activity of sulfated paramylon.

    abstract：:Sulfated derivatives of paramylon, a water-insoluble (1-3)-beta-D-glucan from Euglena gracilis, significantly inhibited the cytopathic effect of human immunodeficiency virus (HIV-1, HIV-2) and the expression of HIV antigen in cultured MT-4, MOLT-4 cells and human peripheral blood mononuclear cells. Native paramylon, N...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(93)90063-o

    authors： Koizumi N,Sakagami H,Utsumi A,Fujinaga S,Takeda M,Asano K,Sugawara I,Ichikawa S,Kondo H,Mori S

    更新日期：1993-05-01 00:00:00

  • Intravascular distribution of zidovudine: role of plasma proteins and whole blood components.

    abstract：:Knowledge of drug protein-binding and blood cell partitioning may be important for evaluating the pharmacokinetic parameters of zidovudine, particularly because of its intracellular site of action and potential to induce side effects. Equilibrium dialysis studies of zidovudine were performed over 2 h to identify the e...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(93)90032-e

    authors： Luzier A,Morse GD

    更新日期：1993-07-01 00:00:00

  • Antiviral action of 5-amino-2-(2-dimethyl-aminoethyl)benzo-[de]-isoquinolin-1,3-dion e.

    abstract：:A newly synthesized imide derivative of 3-nitro-1,8-naphthalic acid, 5-amino-2-(2-dimethylaminoethyl)benzo-[de]-isoquinolin-1,3-dione (designated M-FA-142), was tested on chick embryo cells against herpes simplex virus type 1 (HSV-1) and vaccinia virus (VV), and on Vero cells against African swine fever virus (ASFV). ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(84)90018-4

    authors： García Gancedo A,Gil-Fernández C,Vilas P,Pérez S,Rodriguez F,Paez E,Braña MF,Roldán CM

    更新日期：1984-08-01 00:00:00

  • Infection of neuroblastoma cells by rabies virus is modulated by the virus titer.

    abstract：:Rabies is a lethal viral infection that can affect almost all mammals, including humans. To better understand the replication of Rabies lyssavirus, we investigated if the viral load in brains naturally infected with rabies influences viral internalization and viral growth kinetics in neuroblastoma cells, and if the vi...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2017.11.003

    authors： Fuoco NL,Dos Ramos Silva S,Fernandes ER,Luiz FG,Ribeiro OG,Katz ISS

    更新日期：2018-01-01 00:00:00

  • In vitro characterization of the anti-human cytomegalovirus activity of PMEA (Adefovir).

    abstract：:PMEA [9-[2-(phosphonomethoxy)ethyl]adenine; adefovir] has shown anti-cytomegalovirus activity in animal models and in preliminary human trials. PMEA diphosphate (PMEApp), the active antiviral metabolite of PMEA, is a potent inhibitor of human cytomegalovirus (HCMV) DNA polymerase. PMEA is efficiently taken up and phos...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(97)00050-8

    authors： Xiong X,Flores C,Fuller MD,Mendel DB,Mulato AS,Moon K,Chen MS,Cherrington JM

    更新日期：1997-11-01 00:00:00

  • The effects of antirhino- and enteroviral vinylacetylene benzimidazoles on cytochrome P450 function and hepatic porphyrin levels in mice.

    abstract：:In an ongoing effort to identify an orally bioavailable compound for the treatment of rhino- and enteroviral infections, a series of vinylacetylene benzimidazoles was recently examined. Previous studies demonstrated the potential for these compounds to possess both good in vitro antiviral activity as well as acceptabl...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(99)00013-3

    authors： Tebbe MJ,Jensen CB,Spitzer WA,Franklin RB,George MC,Phillips DL

    更新日期：1999-05-01 00:00:00

  • An inhibitor of the epidermal growth factor receptor function does not affect the ability of human papillomavirus 11 to form warts in the xenografted immunodeficient mouse model.

    abstract：:Epidermal growth factor receptor (EGFr) has been shown to be induced and activated in cells infected with HPV, suggesting that it may play a physiological role in viral replication or in the formation or maintenance of warts. To investigate this possibility, human foreskin tissue was infected with HPV11 and transplant...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2006.12.004

    authors： Parkinson T,Howett MK,Welsh PA,Patrick SD,Neely EB,Flanagan N,Pollack VA,Pustilnik LR,Moyer J,Perros M

    更新日期：2007-04-01 00:00:00

  • High-throughput screening for negative-stranded hemorrhagic fever viruses using reverse genetics.

    abstract：:Viral hemorrhagic fevers (VHFs) cause thousands of fatalities every year, but the treatment options for their management remain very limited. In particular, the development of therapeutic interventions is restricted by the lack of commercial viability of drugs targeting individual VHF agents. This makes approaches lik...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2019.104569

    authors： Wendt L,Bostedt L,Hoenen T,Groseth A

    更新日期：2019-10-01 00:00:00

  • Peptidomimetic furin inhibitor MI-701 in combination with oseltamivir and ribavirin efficiently blocks propagation of highly pathogenic avian influenza viruses and delays high level oseltamivir resistance in MDCK cells.

    abstract：:Antiviral medication is used for the treatment of severe influenza infections, of which the neuraminidase inhibitors (NAIs) are the most effective drugs, approved so far. Here, we investigated the antiviral efficacy of the peptidomimetic furin inhibitor MI-701 in combination with oseltamivir carboxylate and ribavirin ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2015.05.006

    authors： Lu Y,Hardes K,Dahms SO,Böttcher-Friebertshäuser E,Steinmetzer T,Than ME,Klenk HD,Garten W

    更新日期：2015-08-01 00:00:00

  • In vitro antiviral activity of polyoxomolybdates. Mechanism of inhibitory effect of PM-104 (NH4)12H2(Eu4(MoO4)(H2O)16(Mo7O24)4).13H2O on human immunodeficiency virus type 1.

    abstract：:A screening for inhibitors of human immunodeficiency virus type 1 (HIV-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of HIV-1 in CD4+ human MT-4 cells. A novel heteropolyoxomolybdate named PM-104 with the che...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(93)90075-t

    authors： Inouye Y,Tokutake Y,Yoshida T,Seto Y,Hujita H,Dan K,Yamamoto A,Nishiya S,Yamase T,Nakamura S

    更新日期：1993-04-01 00:00:00

  • Natural killer cell inhibits human immunodeficiency virus replication in chronically infected immune cells.

    abstract：:Natural killer (NK) cells are a crucial component of the host innate immune system. We investigated the noncytolytic anti-human immunodeficiency virus (HIV) activity of NK cells in chronically HIV-infected immune cells. Supernatants collected from NK cell cultures (both primary NK cells and NK cell lines, YTS and NK 9...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2006.08.006

    authors： Zhang T,Li Y,Wang YJ,Wang X,Young M,Douglas SD,Ho WZ

    更新日期：2007-02-01 00:00:00

  • Mesuol, a natural occurring 4-phenylcoumarin, inhibits HIV-1 replication by targeting the NF-kappaB pathway.

    abstract：:Coumarins and structurally related compounds have been recently shown to inhibit replication of human immunodeficiency virus (HIV) and thus, exhibit a therapeutic potential. In this study we report that mesuol and isomesuol, two 4-phenyl coumarins, isolated from the tree Marila pluricostata, suppress HIV-1 replication...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2005.02.006

    authors： Márquez N,Sancho R,Bedoya LM,Alcamí J,López-Pérez JL,Feliciano AS,Fiebich BL,Muñoz E

    更新日期：2005-06-01 00:00:00

  • Antiviral treatment efficiently inhibits chikungunya virus infection in the joints of mice during the acute but not during the chronic phase of the infection.

    abstract：:Favipiravir (T-705) is a broad spectrum antiviral which has been approved in Japan for the treatment of severe influenza virus infections. We reported earlier that favipiravir inhibits the in vitro replication of CHIKV and protects against disease progression in CHIKV-infected immunodeficient mice. We here explored wh...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2017.09.016

    authors： Abdelnabi R,Jochmans D,Verbeken E,Neyts J,Delang L

    更新日期：2018-01-01 00:00:00

  • Complete inhibition of viral breakthrough by combination of MKC-442 with AZT during a long-term culture of HIV-1 infected cells.

    abstract：:We have investigated viral breakthrough during a long-term culture of HIV-1-infected cells with the non-nucleoside reverse transcriptase inhibitors (NNRTIs) 6-benzyl-1-ethoxymethyl-5-isopropyluracil (MKC-442), nevirapine and loviride (alpha-APA). When the compounds were examined for their inhibitory effects on HIV-1 (...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(96)00946-1

    authors： Okamoto M,Makino M,Yamada K,Nakade K,Yuasa S,Baba M

    更新日期：1996-06-01 00:00:00

  • Dead-end complexes contribute to the synergistic inhibition of HIV-1 RT by the combination of rilpivirine, emtricitabine, and tenofovir.

    abstract：:The single tablet regimen of the nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs) tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), and the non-nucleoside reverse transcriptase inhibitor (NNRTI) rilpivirine (RPV) is approved for the treatment of HIV-1 infection in treatment-naïve adults. Previous...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.11.010

    authors： Kulkarni R,Feng JY,Miller MD,White KL

    更新日期：2014-01-01 00:00:00

  • Identification of small molecule inhibitors of the Chikungunya virus nsP1 RNA capping enzyme.

    abstract：:Chikungunya virus (CHIKV) is an arthropod-borne alphavirus. Alphaviruses are positive strand RNA viruses that require a 5' cap structure to direct translation of the viral polyprotein and prevent degradation of the viral RNA genome by host cell nucleases. Formation of the 5' RNA cap is orchestrated by the viral protei...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2018.03.013

    authors： Feibelman KM,Fuller BP,Li L,LaBarbera DV,Geiss BJ

    更新日期：2018-06-01 00:00:00

  • Cyclocreatine (1-carboxymethyl-2-iminoimidazolidine) inhibits the replication of human herpes viruses.

    abstract：:The creatine kinase/creatine phosphate (CK/CrP) system plays an important role in cellular energy homeostasis. CK isoenzymes, which reversibly generate ATP from CrP, are compartmentalized at cellular sites where energy is produced or utilized. It has been noted that the expression of CK is induced in cells infected by...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(94)90018-3

    authors： Lillie JW,Smee DF,Huffman JH,Hansen LJ,Sidwell RW,Kaddurah-Daouk R

    更新日期：1994-04-01 00:00:00

  • The use of sialidase therapy for respiratory viral infections.

    abstract：:DAS181 is an inhaled bacterial sialidase which functions by removing sialic acid (Sia) from the surface of epithelial cells, preventing attachment and subsequent infection by respiratory viruses that utilize Sia as a receptor. DAS181 is typical of bacterial sialidases in cleaving Sia α2-3 and Sia α2-6 linkages, and it...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2013.04.012

    authors： Nicholls JM,Moss RB,Haslam SM

    更新日期：2013-06-01 00:00:00

  • Protection of a novel epitope-RNA VLP double-effective VLP vaccine for foot-and-mouth disease.

    abstract：:Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. Previously, we found that the epitope peptide EP141-160 displayed on virus-like particles (VLP) for use as a vaccine showed high immunoreactivity and conferred partially effective protection to animals. In this study, we first ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2016.08.020

    authors： Dong YM,Cai JC,Chen HT,Chen L

    更新日期：2016-10-01 00:00:00

  • An attenuated EIAV vaccine strain induces significantly different immune responses from its pathogenic parental strain although with similar in vivo replication pattern.

    abstract：:The EIAV (equine infectious anemia virus) multi-species attenuated vaccine EIAV(DLV121) successfully prevented the spread of equine infectious anemia (EIA) in China in the 1970s and provided an excellent model for the study of protective immunity to lentiviruses. In this study, we compared immune responses induced by ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2011.08.016

    authors： Lin YZ,Shen RX,Zhu ZY,Deng XL,Cao XZ,Wang XF,Ma J,Jiang CG,Zhao LP,Lv XL,Shao YM,Zhou JH

    更新日期：2011-11-01 00:00:00

  • Altered monocyte response to the dengue virus in those with varying severity of past dengue infection.

    abstract：OBJECTIVE:We sought to investigate the differences in monocyte immune responses to the dengue virus (DENV) in those who previously had either severe disease (past SD) or non-severe dengue (past NSD) following a secondary dengue infection. METHOD:Monocytes from healthy individuals who had either past SD (n = 6) or past...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2019.104554

    authors： Kamaladasa A,Gomes L,Wijesinghe A,Jeewandara C,Toh YX,Jayathilaka D,Ogg GS,Fink K,Malavige GN

    更新日期：2019-09-01 00:00:00

  • Impact of RNA polymerase I inhibitor CX-5461 on viral kinase-dependent and -independent cytomegalovirus replication.

    abstract：:Human cytomegalovirus (HCMV) infections cause congenital birth defects and disease in immunosuppressed individuals. Antiviral compounds can control infection yet their use is restricted due to concerns of toxicity and the emergence of drug resistant strains. We have evaluated the impact of an RNA Polymerase I (Pol I) ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2018.02.014

    authors： Westdorp KN,Terhune SS

    更新日期：2018-05-01 00:00:00