Abstract：
:The zebrafish enjoys several advantages over other model organisms. It is small, easy to maintain, prolific, and numerous genetic tools are available for it. For example, forward genetic screens have allowed investigators to identify important genes potentially involved in a variety of functions from embryogenesis to cancer. However, despite its sophisticated behavioral repertoire, behavioral methods have rarely been utilized in forward genetic screens. Here, we employ a two-tiered strategy, a proof of concept study, to explore the feasibility of behavioral screens. We generated mutant lines using transposon-based insertional mutagenesis, allowing us to bias mutant selection with target genes expressed within the brain. Furthermore, we employed an efficient and fast behavioral pre-selection in which we investigated the locomotory response of 5-day post-fertilization old larval fish to hyperosmotic shock. Based on this assay, we selected five lines for our lower throughput secondary adult behavioral screen. The latter screen utilized tests in which computer animated image presentation and video-tracking-based automated quantification of behavior allowed us to compare heterozygous zebrafish with their wild-type siblings on their responses to a variety of stimuli. We found significant mutation induced adult behavioral alterations in 4 out of the 5 lines analyzed, including changes in response to social or fear inducing stimuli, to handling and novelty, or in habituation to novelty. We discuss the pros and cons of behavioral phenotyping and of the use of different forward genetic methods in biomedical research with zebrafish.
journal_name
Behav Genetjournal_title
Behavior geneticsauthors
Gerlai R,Poshusta TL,Rampersad M,Fernandes Y,Greenwood TM,Cousin MA,Klee EW,Clark KJdoi
10.1007/s10519-016-9818-ysubject
Has Abstractpub_date
2017-01-01 00:00:00pages
125-139issue
1eissn
0001-8244issn
1573-3297pii
10.1007/s10519-016-9818-yjournal_volume
47pub_type
杂志文章abstract：:The most common alternative to a genetic explanation of left-handedness is that sinistrality arises because of birth stress factors. In a sample of 1398 subjects, the association between birth stress and left-handedness was confirmed. More importantly, it was found that left-handed mothers are more likely to have birt...
journal_title：Behavior genetics
pub_type： 杂志文章
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abstract：:Extreme sampling of sibling pairs has been shown to be efficient in terms of statistical power and sample sizes (in number of sibling pairs needed to genotype) to detect a quantitative trait locus (QTL) when the residual distribution is normal. In the present study, the efficiency of extreme sampling strategies to det...
journal_title：Behavior genetics
pub_type： 杂志文章
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abstract：:Heritability estimates of birth weight have been inconsistent. Possible explanations are heritability changes during gestational age or the influence of covariates (e.g. chorionicity). The aim of this study was to model birth weights of twins across gestational age and to quantify the genetic and environmental compone...
journal_title：Behavior genetics
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journal_title：Behavior genetics
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更新日期：2020-09-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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journal_title：Behavior genetics
pub_type： 杂志文章
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journal_title：Behavior genetics
pub_type： 杂志文章,评审
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更新日期：1997-07-01 00:00:00
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journal_title：Behavior genetics
pub_type： 已发布勘误
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更新日期：2019-05-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/BF01066331
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journal_title：Behavior genetics
pub_type： 杂志文章,评审
doi：10.1007/s10519-019-09950-1
更新日期：2019-05-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1023/a:1002090627601
更新日期：2000-01-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/s10519-010-9385-6
更新日期：2011-03-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/BF01065820
更新日期：1991-05-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：2002-03-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：2001-01-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/s10519-006-9122-3
更新日期：2007-01-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/BF01067350
更新日期：1979-11-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/s10519-019-09961-y
更新日期：2019-09-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/s10519-018-9887-1
更新日期：2018-03-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：2011-03-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：1990-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-09-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：1975-07-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章,评审
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更新日期：2003-11-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：1992-07-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/BF01065597
更新日期：1980-07-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
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更新日期：1996-03-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/s10519-007-9183-y
更新日期：2008-03-01 00:00:00
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journal_title：Behavior genetics
pub_type： 杂志文章
doi：10.1007/BF01068210
更新日期：1979-07-01 00:00:00
