Magnetic resonance spectroscopic analysis of neurometabolite changes in the developing rat brain at 7T.


:We utilized proton magnetic resonance spectroscopy to evaluate the metabolic profile of the hippocampus and anterior cingulate cortex of the developing rat brain from postnatal days 14-70. Measured metabolite concentrations were modeled using linear, exponential, or logarithmic functions and the time point at which the data reached plateau (i.e. when the portion of the data could be fit to horizontal line) was estimated and was interpreted as the time when the brain has reached maturity with respect to that metabolite. N-acetyl-aspartate and myo-inositol increased within the observed period. Gluthathione did not vary significantly, while taurine decreased initially and then stabilized. Phosphocreatine and total creatine had a tendency to increase towards the end of the experiment. Some differences between our data and the published literature were observed in the concentrations and dynamics of phosphocreatine, myo-inositol, and GABA in the hippocampus and creatine, GABA, glutamine, choline and N-acetyl-aspartate in the cortex. Such differences may be attributed to experimental conditions, analysis approaches and animal species. The latter is supported by differences between in-house rat colony and rats from Charles River Labs. Spectroscopy provides a valuable tool for non-invasive brain neurochemical profiling for use in developmental neurobiology research. Special attention needs to be paid to important sources of variation like animal strain and commercial source.


Brain Res


Brain research


Ramu J,Konak T,Liachenko S




Has Abstract


2016-11-15 00:00:00












  • Effect of thyrotropin releasing hormone on U-50,488H-induced pharmacological responses in mice.

    abstract::The effect of thyrotropin releasing hormone (TRH) administered either subcutaneously (s.c.) or intracerebroventricularly (i.c.v.) on the analgesic and hypothermic actions of U-50,488H, a highly selective kappa-opiate agonist, was determined in male Swiss Webster mice. Intraperitoneal administration of U-50,488H (8-32 ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Bhargava HN,Thorat SN

    更新日期:1993-10-15 00:00:00

  • Effects of prefrontal cortex and hippocampal NMDA NR1-subunit deletion on complex cognitive and social behaviors.

    abstract::Glutamate N-methyl-D-aspartate receptors (NMDARs) in the medial prefrontal cortex (mPFC) and hippocampus may play an integral role in complex cognitive and social deficits associated with a number of psychiatric illnesses including autism, mood disorders, and schizophrenia. We used localized infusions of adeno-associa...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Finlay JM,Dunham GA,Isherwood AM,Newton CJ,Nguyen TV,Reppar PC,Snitkovski I,Paschall SA,Greene RW

    更新日期:2015-03-10 00:00:00

  • Synaptogenesis in the rat suprachiasmatic nucleus: a light microscopic immunocytochemical survey.

    abstract::MabQ155, a monoclonal antibody against synaptophysin, has been used to conduct a light microscopic survey of synaptogenesis in the suprachiasmatic nucleus of the perinatal rat. Synaptophysin is an integral component of synaptic vesicle membranes which is expressed in growth cones and growth cone filopodia as well as i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Laemle LK,Repke KB,Hawkes R,Rice FL

    更新日期:1991-03-22 00:00:00

  • Cholinergic modulation of the functional organization of the cat visual cortex.

    abstract::The cortex receives a cholinergic input which is considered to be involved in mediating the effects of arousal. The experiments reported here have examined the nature of the cholinergic influence on the neuronal organization of the cat visual cortex. Out of 83 cells studied, 92% exhibited a modification in their visua...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Sillito AM,Kemp JA

    更新日期:1983-12-19 00:00:00

  • Cholinergic modulation of neocortical long-term potentiation in the awake, freely moving rat.

    abstract::The neocortex has proven resistant to LTP induction using standard in vitro and acute, in vivo preparations. Because the neocortex is widely thought to be involved in long-term information storage, this resistance raises questions about the validity of LTP as a memory model. Recently, we have shown that the neocortex ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Boyd TE,Trepel C,Racine RJ

    更新日期:2000-10-20 00:00:00

  • In vivo chronic lead exposure alters [3H]nitrendipine binding in rat striatum.

    abstract::The effect of lead as a neurotoxic agent has been associated with alterations in calcium metabolism. On this line, the present study shows that lead alters the characteristics of [3H]nitrendipine ([ 3H]NDP) binding to rat striatal membranes. In vitro, lead shares the action of calcium in enhancing [3H]NDP binding alth...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Rius RA,Lucchi L,Govoni S,Trabucchi M

    更新日期:1984-11-19 00:00:00

  • Lesions and reversible inactivation of the dorsolateral caudate-putamen impair cocaine-primed reinstatement to cocaine-seeking in rats.

    abstract::Recent evidence suggests that cocaine addiction may involve progressive drug-induced neuroplasticity of the dorsal striatum. Here, we examined the effects of a) dorsolateral caudate putamen (dlCPu) lesions on cocaine self-administration, extinction of responding, and subsequent reinstatement to cocaine-seeking, and b)...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Gabriele A,See RE

    更新日期:2011-10-12 00:00:00

  • Stereospecific acute neuronotoxicity of 'uncommon' plant amino acids linked to human motor-system diseases.

    abstract::The L-isomer of beta-N-methylamino-L-alanine (BMAA), present in free form in seed of Cycas circinalis, elicits in spinal cord cultures a pattern of acute postsynaptic neuronal vacuolation comparable to that induced by beta-N-oxalylamino-L-alanine (BOAA), an excitotoxic amino acid of greater potency isolated from seed ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Nunn PB,Seelig M,Zagoren JC,Spencer PS

    更新日期:1987-05-05 00:00:00

  • Selective opioid agonist and antagonist competition for [3H]-naloxone binding in amphibian spinal cord.

    abstract::Opioids elicit antinociception in mammals through three distinct types of receptors designated as mu, kappa and delta. However, it is not clear what type of opioid receptor mediates antinociception in non-mammalian vertebrates. Radioligand binding techniques were employed to characterize the site(s) of opioid action i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Newman LC,Wallace DR,Stevens CW

    更新日期:2000-11-24 00:00:00

  • Adult human bone marrow stromal spheres express neuronal traits in vitro and in a rat model of Parkinson's disease.

    abstract::Adult human bone marrow stromal cells (hMSCs) grown in suspension culture gave rise to spheres of neural progenitor (NP) cells, capable of expressing both dopaminergic (DA) and GABAergic (GABA) traits. After transplantation into the Parkinsonian rat, human NPs and neurons were present at 2 weeks. Although no DA neuron...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Suon S,Yang M,Iacovitti L

    更新日期:2006-08-23 00:00:00

  • Dispreferred adjective orders elicit brain responses associated with lexico-semantic rather than syntactic processing.

    abstract::We examined how adjective ordering is used in language comprehension by crossing order preference and concreteness in phrases consisting of two adjectives and a noun. We used both more typical phrases in which the preferred order has a concrete second adjective ("exhaustive hardback encyclopedia") and those with a con...

    journal_title:Brain research

    pub_type: 杂志文章,随机对照试验


    authors: Huang HW,Federmeier KD

    更新日期:2012-09-26 00:00:00

  • Behavioural responses of bicucculline methiodide injections into the ventral hypothalamus of freely moving, socially interacting rats.

    abstract::Several studies, using electrical stimulation of parts of the hypothalamus, have shown, that different parts of the hypothalamus yield different behavioural responses upon stimulation. In order to differentiate between stimulation of neuronal cell bodies and passing fibres and to investigate the role of GABA in hypoth...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Roeling TA,Kruk MR,Schuurmans R,Veening JG

    更新日期:1993-06-25 00:00:00

  • The effect of a conditioning lesion on sudomotor axon regeneration.

    abstract::The effects of a conditioning lesion on the rate of sudomotor axon regeneration were judged by the recovery of sweat gland (SG) secretion after cholinergic stimulation. Three groups of mice were given a conditioning lesion by crushing the sciatic nerve at mid-thigh 4, 7, and 14 days before a test lesion. A 4th group r...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Navarro X,Kennedy WR

    更新日期:1990-02-19 00:00:00

  • Quantitative analysis of glutamic acid decarboxylase-immunoreactive neurons in the anterior thalamus of the human brain.

    abstract::Local circuit neurons in the human anterior thalamus (AT) were identified on the basis of glutamic acid decarboxylase immunoreactivity (GAD-IR). GAD-IR neurons of the AT displayed small diameter somas with thin, sparsely-branching dendrites, consistent with the morphological characteristics of local circuit neurons fo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Dixon G,Harper CG

    更新日期:2001-12-27 00:00:00

  • Divergent projections of catecholaminergic neurons in the nucleus of the solitary tract to limbic forebrain and medullary autonomic brain regions.

    abstract::The nucleus of the solitary tract (NTS) is a critical structure involved in coordinating autonomic and visceral activities. Previous independent studies have demonstrated efferent projections from the NTS to the nucleus paragigantocellularis (PGi) and the central nucleus of the amygdala (CNA) in rat brain. To further ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Reyes BA,Van Bockstaele EJ

    更新日期:2006-10-30 00:00:00

  • Red cell velocity and autoregulation in the cerebral cortex of the cat.

    abstract::This report deals with a comparison of total and regional cerebral blood flow with red cell velocity in the microcirculation of the ecto- and suprasylvian areas of the cerebral cortex of the cat and their response to changes in systemic blood pressure. Total and regional blood flows were measured during a steady-state...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Chang BL,Santillan G,Bing RJ

    更新日期:1984-08-06 00:00:00

  • Injections of D-amphetamine into the ventral pallidum increase locomotor activity and responding for conditioned reward: a comparison with injections into the nucleus accumbens.

    abstract::The nucleus accumbens and ventral pallidum receive dopamine (DA) projections from the mesencephalon. Although DA inputs to the nucleus accumbens are implicated in both locomotion and reward processes, little is known of the behavioural significance of DA in the ventral pallidum. These studies examined the effects of D...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Fletcher PJ,Korth KM,Sabijan MS,DeSousa NJ

    更新日期:1998-09-14 00:00:00

  • Effects of low intensity radiofrequency electromagnetic fields on electrical activity in rat hippocampal slices.

    abstract::Slices of rat hippocampus were exposed to 700 MHz continuous wave radiofrequency (RF) fields (25.2-71.0 V m(-1), 5-15 min exposure) in a stripline waveguide. At low field intensities, the predominant effect on the electrically evoked field potential in CA1 was a potentiation of the amplitude of the population spike by...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Tattersall JE,Scott IR,Wood SJ,Nettell JJ,Bevir MK,Wang Z,Somasiri NP,Chen X

    更新日期:2001-06-15 00:00:00

  • Involvement of TNF-alpha in glutamate-induced apoptosis in a differentiated neuronal cell line.

    abstract::We examined the involvement of tumor necrosis factor (TNF)-alpha on glutamate-induced cytotoxicity in a differentiated neuronal cell line. In this study, we used nerve growth factor (NGF)-differentiated PC12h cells. Glutamate cytotoxicity was assessed using the MTS and TUNEL assays. To detect TNF-alpha levels in cultu...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kogo J,Takeba Y,Kumai T,Kitaoka Y,Matsumoto N,Ueno S,Kobayashi S

    更新日期:2006-11-29 00:00:00

  • Cell division in the developing cat retina occurs in two zones.

    abstract::Cytogenesis in the kitten retina has been investigated with [3H]thymidine autoradiography and a stain for mitotic figures. The nuclei of cells in S-phase are located adjacent to the inner margin of the cytoblast layer, near the inner plexiform layer. The nuclei then migrate toward the outer limiting membrane (OLM) to ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Robinson SR,Rapaport DH,Stone J

    更新日期:1985-03-01 00:00:00

  • Early social isolation disrupts latent inhibition and increases dopamine D2 receptor expression in the medial prefrontal cortex and nucleus accumbens of adult rats.

    abstract::Adolescence is a critical period for neurodevelopment. In the present study, we investigated the effects of peri-adolescent social isolation on latent inhibition (LI) and dopamine D2 receptor expression in the medial prefrontal cortex (mPFC) and nucleus accumbens (NAc) of young adult rats. Male Sprague-Dawley rats wer...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Han X,Li N,Xue X,Shao F,Wang W

    更新日期:2012-04-04 00:00:00

  • Brain neuropeptide Y in the control of adrenocorticotropic hormone secretion in the dog.

    abstract::An immunoneutralization technique with specific antibodies was used to explore the role of endogenous neuropeptide Y (NPY) in the adrenocorticotropic hormone (ACTH) release after hypoglycemic stress in the dog. Dogs received injections of rabbit antihuman NPY gamma-globulin (anti-NPY) or normal gamma-globulin (NGG) in...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Inui A,Inoue T,Nakajima M,Okita M,Sakatani N,Okimura Y,Chihara K,Baba S

    更新日期:1990-03-05 00:00:00

  • Metabotropic glutamate receptor/phospholipase C system in female rat heart.

    abstract::Glutamate is the main excitatory neurotransmitter in the central nervous system. This amino acid mediates learning and memory processes acting through ionotropic and metabotropic receptor binding. Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that stimulate phospholipase C (PLC) or inhibit ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Iglesias I,Castillo CA,León D,Ruíz MA,Albasanz JL,Martín M

    更新日期:2007-06-11 00:00:00

  • Dose-independent antinociceptive interaction of endogenous ligands at the spinal level.

    abstract::Adenosine, agmatine and kynurenic acid are endogenous ligands acting on different (e.g. adenosine, NMDA, alpha(2)-adrenergic and imidazoline) receptors with a potential role in nociception at the spinal level. Their antinociceptive effects have already been investigated as monotherapy, but only a few studies have repo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kekesi G,Joo G,Csullog E,Peter-Szabo M,Benedek G,Horvath G

    更新日期:2004-12-10 00:00:00

  • Some electrophysiological and pharmacological properties of the cortical, noradrenergic projection of the locus coeruleus in the rat.

    abstract::The effect of repetitive stimulation of the locus coeruleus (LC) on the discharge rate of spontaneously active neurons of the visual, rostral and cingulate cortex was investigated in untreated and catecholamine-depleted rats under chloral hydrate anesthesia. In untreated animals, the inhibitory transsynaptic effects p...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Olpe HR,Glatt A,Laszlo J,Schellenberg A

    更新日期:1980-03-17 00:00:00

  • Sex differences in insular functional connectivity in response to noxious visceral stimulation in rats.

    abstract::Insular cortex (INS) plays a critical role in pain processing and shows sex differences in functional activation during noxious visceral stimulation. Less is known regarding functional interactions within the INS and between this structure and other parts of the brain. Cerebral blood flow mapping was performed using [...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wang Z,Guo Y,Mayer EA,Holschneider DP

    更新日期:2019-08-15 00:00:00

  • Afferents to the central nucleus of the amygdala and functional subdivisions of the periaqueductal gray: neuroanatomical substrates for affective behavior.

    abstract::Evidence suggests the periaqueductal gray (PAG) is involved in the integration of behavioral and autonomic components of affective behavior. Our laboratory has shown that electrical stimulation of the ventrolateral periaqueductal gray (vl PAG) versus the dorsolateral periaqueductal gray (dl PAG), in the rabbit, elicit...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Paredes J,Winters RW,Schneiderman N,McCabe PM

    更新日期:2000-12-22 00:00:00

  • Ca(2+)-dependent release of [3H]GABA in cultured chick retina cells.

    abstract::Depolarization by K+ (50 mM) of cultured chick retina cells released 1.14 +/- 0.28% of the accumulated [3H] gamma-aminobutyric acid (GABA) in the absence of Ca2+, but when 1.0 mM Ca2+ was present, the internal free calcium ion concentration [Ca2+]i rose by about 750 nM and the [3H]GABA release about doubled to a value...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Duarte CB,Ferreira IL,Santos PF,Oliveira CR,Carvalho AP

    更新日期:1992-09-18 00:00:00

  • Changes in rapidly transported proteins in the auditory nerve after hair cell loss.

    abstract::In an effort to further characterize the proteins of the auditory nerve, the effects of hair cell loss on rapidly transported proteins of the auditory nerve were studied. The effects were studied in the waltzing guinea pig, a genetically deaf animal which displays an age-dependent loss of sensory cells, and in normal ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wenthold RJ,McGarvey ML

    更新日期:1982-12-16 00:00:00

  • Noise exposure enhances auditory cortex responses related to hyperacusis behavior.

    abstract::Hyperacusis, a marked intolerance to normal environmental sound, is a common symptom in patients with tinnitus, Williams syndrome, autism, and other neurologic diseases. It has been suggested that an imbalance of excitation and inhibition in the central auditory system (CAS) may play an important role in hyperacusis. ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Sun W,Deng A,Jayaram A,Gibson B

    更新日期:2012-11-16 00:00:00