Abstract:
:We have characterized the beta-adrenergic receptor binding site and the beta-adrenergic cAMP response of the HL-60 cell. The hydrophilic ligand [3H]-(-)-CGP-12177 was specifically and reversibly bound to one single class of binding sites (Kd 220 pM and Bmax 1,970 sites/cell). The adrenergic agonists inhibited the specific radioligand binding. The order of potency was isoproterenol greater than epinephrine greater than norepinephrine. The beta-2 selective antagonist ICI 118551 had a binding affinity 3 orders of potency higher than the beta-1 selective antagonist, atenolol. The adrenergic agonists elevated the cAMP accumulation in a concentration-dependent mode. The order of potency was isoproterenol greater than epinephrine greater than norepinephrine. Both the binding and the functional studies revealed stereospecificity and reversibility. The present data show that HL-60 cells possess beta-2 adrenergic receptors functionally coupled to adenylate cyclase.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Sager G,Bang BE,Pedersen M,Aarbakke Jdoi
10.1002/jlb.44.1.41subject
Has Abstractpub_date
1988-07-01 00:00:00pages
41-5issue
1eissn
0741-5400issn
1938-3673journal_volume
44pub_type
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