Abstract:
:The oxidative metabolic burst of stimulated human polymorphonuclear leukocytes (PMNs) has been evaluated by the measurement of oxygen consumption, chemiluminescence, and oxygen radicals (O2-, H2O2, OH-) derived from activation of the hexose monophosphate shunt (HMPS). PMNs from patients with chronic granulomatous disease (CGD) are shown to lack functional NADPH oxidase and undetectable oxygen radical generation. However, using single cell analysis by flow cytometry and 2',7'-dichlorofluorescin (DCFH) oxidation by H2O2, significant DCFH oxidation by the PMA stimulated CGD PMNs was observed. Furthermore, 1mM potassium cyanide enhanced DCFH oxidation by control and CGD PMNs. DCFH oxidation by cells from an obligate heterozygous mother of an X-linked CGD patient was intermediate. These observations suggest that a PMA induced oxidase enzyme is present in CGD cells.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Hassan NF,Campbell DE,Douglas SDdoi
10.1002/jlb.43.4.317subject
Has Abstractpub_date
1988-04-01 00:00:00pages
317-22issue
4eissn
0741-5400issn
1938-3673journal_volume
43pub_type
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pub_type: 杂志文章
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