Abstract:
:A simple and rapid method for the purification of morphologically and functionally intact eosinophils from human blood of both normal and eosinophilic subjects is described. The method is based on a single centrifugation of total blood leukocytes suspended in Percoll with specific gravity 1.0853 g/ml, after erythrocyte removal by dextran sedimentation. The peculiarity of this isolation technique is the maintenance of strictly physiological values of pH and osmolality throughout the entire procedure. Moreover, the cells are not subjected to measures aimed at changing the physical properties of either neutrophils or eosinophils. Because of such characteristics, this isolation method could be usefully exploited for comparative studies of normal and eosinophilic normodense eosinophils and of neutrophils and eosinophils from the same noneosinophilic subject.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Cramer R,Dri P,Zabucchi G,Patriarca Pdoi
10.1002/jlb.52.3.331subject
Has Abstractpub_date
1992-09-01 00:00:00pages
331-6issue
3eissn
0741-5400issn
1938-3673journal_volume
52pub_type
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