Abstract:
:CD8(+) T cells play an important role in immune regulation and effective immune responses against tumor cells, viral infection, and intracellular pathogens. In this report, using tiger or 10BiT mice, we defined a population of IL-10-producing CD8(+) T cells that were induced by IL-4. These IL-10(+)CD8(+) T cells possessed a strong inhibitory effect on the CD4(+) T cell proliferation in an IL-10-dependent and cell contact-dependent fashion. In comparison with IL-10(-)CD8(+) T cells, IL-10(+)CD8(+) T cells expressed an array of Th2-like cytokines (IL-4, IL-5), perforin, and granzymes, as well as the cell cycle regulatory protein Cdkn2a. Interestingly, knockdown of cdkn2a using siRNA reduced IL-4-induced IL-10 production significantly. Furthermore, CD8(+) T cells from Cdkn2a(-/-) mice produced a significantly lower amount of IL-10, and the effect was limited to CD8(+) T cells but not observed in CD4(+) T cells and APCs. Finally, IL-10(+)CD8(+) T cells played a protective role in the TNBS-induced murine colitis model, indicating a critical role of this population of CD8(+) T cells in regulatory immune responses. Taken together, we have defined a population of IL-10-producing CD8(+) Tregs induced by IL-4 and mediated by Cdkn2a.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Zhao Y,Zhao H,Sun Y,Hao J,Qi X,Zhou X,Wu Z,Wang P,Kaech SM,Weaver CT,Flavell RA,Zhao L,Yao Z,Yin Zdoi
10.1189/jlb.0213064subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1103-12issue
6eissn
0741-5400issn
1938-3673pii
jlb.0213064journal_volume
94pub_type
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