Abstract:
:In an analysis of nitric oxide (.NO) production and toxicity, chicken macrophage-generated .NO inhibited mitochondrial activity in both .NO-producing macrophages themselves and lymphoid tumor targets. However, differences in targeting of mitochondrial toxicity were observed among these cells. Two chicken macrophage cell lines, HD11 and MQ-NCSU, produced .NO (measured as nitrite) dependent upon concentrations of L-arginine and bacterial endotoxin (lipopolysaccharide). Mitochondrial activity was negatively correlated with the amount of .NO produced. Using a modified MTT assay, .NO induced suppression in two mitochondrial complexes. Mitochondrial activity was significantly suppressed among HD11 cells receiving LPS alone (complex I, 63.0 +/- 5.5% suppression; complex II, 27.9 +/- 5.2%). In contrast, mitochondrial activities in samples receiving LPS plus inhibitor, NG-nitro-L-arginine methyl ester (NAME; 5 mM) or 2,4-diamino-6-hydroxypyrimidine (DAHP; 5 mM), were not significantly different from control values. When HD11 macrophages were cocultured with lymphoblastoid tumor targets, RECC-CU60 (T cell) or LSCC-RP9 (B cell), adding LPS (1 microgram/ml), tumor cell mitochondrial activity was significantly suppressed. In the generator macrophages, complex I was more suppressed than complex II, whereas in lymphoid targets no such difference was observed. These results indicate that .NO inhibits complex I and II mitochondrial activity but that differential targeting can occur among chicken leukocyte populations.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Sung YJ,Dietert RRdoi
10.1002/jlb.56.1.52subject
Has Abstractpub_date
1994-07-01 00:00:00pages
52-8issue
1eissn
0741-5400issn
1938-3673journal_volume
56pub_type
杂志文章abstract::Alpha-defensins are antimicrobial peptides that contribute to innate-immune functions of neutrophils and intestinal Paneth cells. Transcription of alpha-defensin genes occurs early in neutrophilic myelopoeisis. To examine the mechanisms that regulate alpha-defensin gene expression, we analyzed transcription of rat neu...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0803384
更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Journal of leukocyte biology
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abstract::A cell resistant to concentrations of mitomycin-C associated with the inactivation of many in vitro immune responses is described. This cell is responsive to concanavalin A (Con A), poorly adherent to nylon wool, present in relatively greater numbers in lymph node than other lymphoid tissues, and is relatively radiose...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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journal_title:Journal of leukocyte biology
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更新日期:2007-12-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0107071
更新日期:2007-08-01 00:00:00
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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更新日期:1986-05-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0106053
更新日期:2006-12-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::The mechanisms by which concanavalin A (Con A), a lectin, activates T cells are poorly studied. A low dose of Con A is stimulatory for T cells, whereas a high dose of Con A results in suppression of proliferation and enhanced T cell death. The expression and functional roles of costimulatory receptors, CD28 and cytoto...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1104644
更新日期:2005-07-01 00:00:00
abstract::Highly active antiretroviral therapy (HAART) against human immunodeficiency virus type 1 (HIV-1) infection dramatically suppresses viral load, leading to marked reductions in HIV-1 associated morbidity and mortality. However, infected cell reservoirs and low-level replication persist in the face of suppressive HAART, ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0306139
更新日期:2006-11-01 00:00:00
abstract::Although tumor growth enhances macrophage (m phi) cytotoxic activity by increasing their tumor necrosis factor-alpha (TNF-alpha) production, increased prostaglandin E2 (PGE2) synthesis reduces most immune responses during tumor growth. Macrophages that do not express major histocompatibility complex class II molecules...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.53.5.550
更新日期:1993-05-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.6A0114-004RR
更新日期:2014-10-01 00:00:00
abstract::Engagement of 2B4 (CD244) with CD48 results in activation, costimulation, or inhibition of NK cell activities, depending on the cell types and the stage of differentiation. In vivo, 2B4+ NK cells can interact with CD48+ NK cells and also with surrounding CD48+ hematopoietic cells. Similarly, CD48+ NK cells may be trig...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0410198
更新日期:2010-10-01 00:00:00
abstract::Antigen presentation to T lymphocytes has been characterized extensively in terms of T lymphocyte activation and eventual cell death. In contrast, little is known about the consequences of antigen presentation for the antigen-presenting cell (APC). We have determined the outcome of major histocompatibility complex cla...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0703356
更新日期:2004-06-01 00:00:00
abstract::Although a number of chemokine receptors display coreceptor activities in vitro, chemokine receptor 5 (CCR5) and CXC chemokine receptor 4 (CXCR4) remain the major coreceptors used by the human immunodeficiency virus type 1 (HIV-1). In this study, we used an envelope-mediated fusion assay to demonstrate low CCR4 corece...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::Immune exhaustion in T cells significantly impacts their ability to control malignancies and infections, and its discovery has led to revolutionary therapies for cancer in the form of checkpoint blockade. NK cells, like T cells, are lymphocytes that recognize virally infected and malignantly transformed cells. However...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
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journal_title:Journal of leukocyte biology
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doi:
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.5A0116-037R
更新日期:2016-12-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.5A1116-456R
更新日期:2017-07-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.56.4.453
更新日期:1994-10-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0712327
更新日期:2013-02-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that accumulate during pathologic conditions, such as cancer. Patients diagnosed with advanced metastatic cancers have an average survival of 12-24 mo, a survival time that hasn't changed significantly in the past 30 yr. ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
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更新日期:2017-09-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.66.4.634
更新日期:1999-10-01 00:00:00
abstract::Endotoxin (lipopolysaccharide, LPS) is known to induce inflammatory responses, such as monocyte/macrophage adherence, migration, and accumulation. Recruitment and accumulation of macrophages during infection and inflammation are regulated by integrin-mediated cell-extracellular matrix interactions. In the present repo...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.57.4.624
更新日期:1995-04-01 00:00:00