Abstract:
:In our previous research, the formation and development of age-related cataract (ARC) is associated with DNA hypermethylation of some genes in lens epithelial cells (LECs). This study aimed to investigate the expression profile of DNA methylation- and transcriptional repression-associated genes in LECs of ARC. The expression levels of the genes were first evaluated by microarray analysis. The results were further confirmed by Quantitative Real-Time PCR (qRT-PCR) and Western blot assay. The mRNA and protein levels of 5 genes increased in LECs of ARCs compared with the controls. These data provided a global perspective on expression of DNA methylation- and transcriptional repression-associated genes. The study supports the notion that the epigenetic modification of macromolecules in LECs might contribute to ARC pathogenesis.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Wang Y,Zhang G,Kang L,Guan Hdoi
10.1007/s10571-016-0393-9subject
Has Abstractpub_date
2017-04-01 00:00:00pages
537-543issue
3eissn
0272-4340issn
1573-6830pii
10.1007/s10571-016-0393-9journal_volume
37pub_type
杂志文章abstract::1. Radiotherapy has attracted increasing interest in recent years. It is known that ionizing radiation induces oxygen radical injury, whereas oxidative stress by the radiation can cause cellular responses to defense cellular injury. In this study, the metabolism of antioxidants in response to ionizing radiation to the...
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