Abstract:
:1. Spatio-temporal changes in phosphorylated (pNFP) and nonphosphorylated (npNFP) neurofilament proteins were assessed immunocytochemicaly in adult rat cerebellum, 2-30 days following unilateral injection of kainic acid (KA) or physiological saline (s.c.). 2. Analysis of the staining intensity and pattern demonstrated that injection of both KA and physiological saline elicited significant and long-lasting increase of pNFP and npNFP immunoreactivity, at the ipsilateral, and to lesser extent at the contralateral side of lesion. 3. Kainate intoxication induced abundant expression of pNFP and npNFP in cerebellar white matter, as well as in all layers of perilesioned cortex. Higher pNFP expression was evidenced in the Purkinje cell layer, particularly at cell bodies, initial segments, and proximal dendrites, which normally do not contain pNFP. In addition, synaptophysin immunocytochemistry was used as a marker of synaptogenesis and plasticity. 4. Spatio-temporal pattern of NFP and synaptophysin expression suggests that perilesioned cortex undergoes dynamic changes following brain demage and possess a reparative capacity to abridge the consequences of brain trauma.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Milenkovic I,Filipovic R,Nedeljkovic N,Pekovic S,Culic M,Rakic L,Stojiljkovic Mdoi
10.1023/b:cemn.0000022769.44211.2bsubject
Has Abstractpub_date
2004-06-01 00:00:00pages
367-78issue
3eissn
0272-4340issn
1573-6830journal_volume
24pub_type
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