Abstract:
:Spinal cord injury (SCI) remains a formidable challenge in the clinic. In the current study, we examined the effects of the TLX gene on the proliferation and neuronal differentiation of dermal multipotent stem cells (DMSCs) in vitro and the potential of these cells to improve SCI in rats in vivo. DMSCs were stably transfected with TLX-expressing plasmid (TLX/DMSCs). Cell proliferation was examined using the MTT assay, and neuronal differentiation was characterized by morphological observation combined with immunocytochemical/immunofluorescent staining. The in vivo functions of these cells were evaluated by transplantation into rats with SCI, followed by analysis of hindlimb locomotion and post-mortem histology. Compared to parental DMSCs, TLX/DMSCs showed enhanced proliferation and preferential differentiation into NF200-positive neurons in contrast to GFAP-positive astrocytes. When the undifferentiated cells were transplanted into rats with SCI injury, TLX/DMSCs led to significant improvement in locomotor recovery and healing of SCI, as evidenced by reduction in scar tissues and cavities, increase in continuous nerve fibers/axons and enrichment of NF200-positive neurons on the histological level. In conclusion, TLX promotes the proliferation and neuronal differentiation of DMSCs and thus, may serve as a promising therapy for SCI in the clinic.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Wang T,Ren X,Xiong J,Zhang L,Qu J,Xu Wdoi
10.1007/s10571-010-9642-5subject
Has Abstractpub_date
2011-04-01 00:00:00pages
479-87issue
3eissn
0272-4340issn
1573-6830journal_volume
31pub_type
杂志文章abstract::Cannabinoid (CB) ligands have demonstrated neuroprotective properties. In this study we compared the effects of a diverse set of CB ligands against β amyloid-mediated neuronal toxicity and activated microglial-conditioned media-based neurotoxicity in vitro, and compared this with a capacity to directly alter β amyloid...
journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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journal_title:Cellular and molecular neurobiology
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