Feedback regulation of SREBP and aromatase in A beta(25-35)-supplemented human neuroblastoma cells.


:1. The analogies between the processing of amyloid precursor protein (APP) and other transmembrane sterol regulatory element binding proteins (SREBPs) inspired us to conduct further studies on whether beta-amyloid (Abeta) affects aromatase by interacting with APP and SREBP. 2. In this study, cultured human neuroblastoma cells (SHSY-5Y) were incubated in experimental media (media without FBS, the main cholesterol source) in the presence or absence of Abeta (1 microM) for 24 h. 3. Cellular extracts were subjected to immunoblot analysis using anti-APP, anti-aromatase and anti-SREBP-1. In these cell lines, we detected aromatase (55 kDa), SREBP cleavage product (68 kDa) and APP precursor (100-95 kDa) and cleavage product (60 kDa) by immunoblotting. Aromatase and SREBP levels were elevated in the cells incubated 24 h in experimental media and were attenuated in Abeta-supplemented experimental media. 4. The disturbance of cholesterol homeostasis appears to be an important factor in the pathogenesis of Alzheimer's disease. These findings may have important implications for understanding the mechanisms of the aromatase enzyme gene in disease states such as Alzheimer's.


Cell Mol Neurobiol


Kelicen P,Nordberg A




Has Abstract


2006-05-01 00:00:00












  • Deficiency in endothelin receptor B reduces proliferation of neuronal progenitors and increases apoptosis in postnatal rat cerebellum.

    abstract::Endothelins regulate cellular functions in the mammalian brain through the endothelin receptors A and B (EDNRA and EDNRB). In this study, we investigated the role of EDNRB on cell proliferation in the cerebellum by using the spotting lethal (sl) rat, which carries a naturally occurring deletion in the EDNRB gene. Prol...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Vidovic M,Chen MM,Lu QY,Kalloniatis KF,Martin BM,Tan AH,Lynch C,Croaker GD,Cass DT,Song ZM

    更新日期:2008-12-01 00:00:00

  • Increased Expression of 15-Hydroxyprostaglandin Dehydrogenase in Spinal Astrocytes During Disease Progression in a Model of Amyotrophic Lateral Sclerosis.

    abstract::Amyotrophic lateral sclerosis (ALS) is an adult-onset, progressive, and fatal neurodegenerative disease caused by selective loss of motor neurons. Both ALS model mice and patients with sporadic ALS have increased levels of prostaglandin E2 (PGE2). Furthermore, the protein levels of microsomal PGE synthase-1 and cycloo...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Miyagishi H,Kosuge Y,Takano A,Endo M,Nango H,Yamagata-Murayama S,Hirose D,Kano R,Tanaka Y,Ishige K,Ito Y

    更新日期:2017-04-01 00:00:00

  • Effect of cholinomimetics on the release and uptake of L-[3H] glutamic acid in rat neostriatum.

    abstract::The effects of cholinomimetics on the release and uptake of L-glutamic acid have been studied by means of local superfusion of rat neostriatum (in vivo) and using striatum slices (in vitro). Cholinomimetics regulate the release of L-glutamic acid through both muscarinic (m-) and nicotinic (n-) choline receptors accord...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Godukhin OV,Budantsev AYu,Selifanova OV,Agapova VN

    更新日期:1984-06-01 00:00:00

  • The role of nitric oxide in homocysteine thiolactone-induced seizures in adult rats.

    abstract::The role of NO in epileptogenesis has been studied in different experimental models, and the reported results have been highly contradictory. The current study aimed to determine the role of NO in mechanisms of D: ,L: -homocysteine-thiolactone (H) induced seizures by testing the action of L: -arginine (NO precursor) a...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Hrncić D,Rasić-Marković A,Krstić D,Macut D,Djuric D,Stanojlović O

    更新日期:2010-03-01 00:00:00

  • SNAP-25 contains non-acylated thiol pairs that can form intrachain disulfide bonds: possible sites for redox modulation of neurotransmission.

    abstract::Intrachain disulfide bond formation among the cysteine thiols of SNAP-25, a component of the SNARE protein complex required for neurotransmitter release, has been hypothesized to link oxidative stress and inhibition of synaptic transmission. However, neither the availability in vivo of SNAP-25 thiols, which are known ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Foley TD,Clark AR,Stredny ES,Wierbowski BM

    更新日期:2012-03-01 00:00:00

  • Neuroprotective Effect of Natural Alkaloid Fangchinoline Against Oxidative Glutamate Toxicity: Involvement of Keap1-Nrf2 Axis Regulation.

    abstract::Oxidative glutamate toxicity plays a vital role in the neurodegeneration diseases, including Alzheimer's diseases (AD). This study set out with the aim to investigate the beneficial effects of fangchinoline (FAN), a natural alkaloid, against glutamate-induced oxidative damage, and to clarify the underlying cellular an...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Bao F,Tao L,Zhang H

    更新日期:2019-11-01 00:00:00

  • The Tumorgenicity of Glioblastoma Cell Line U87MG Decreased During Serial In Vitro Passage.

    abstract::Established cancer cell lines are routinely used to study cancer. Several factors such as serial passage may affect the reproducibility of experiments with cancer cell lines, but few researches focused on these changes. In the present study, different morphology and decreased tumorigenicity were observed in late passa...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zeng Y,Wang X,Wang J,Yi R,Long H,Zhou M,Luo Q,Zhai Z,Song Y,Qi S

    更新日期:2018-08-01 00:00:00

  • Correlations between cholinesterase activity and cognitive scores in post-ischemic rats and patients with vascular dementia.

    abstract::The biochemical changes such as the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were investigated in rats with global cerebral ischemia and in vascular dementia (VaD) subjects in this study. The AChE activity showed a significant decrease in plasma and a significant increase in the hipp...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Xiao Y,Guan ZZ,Wu CX,Li Y,Kuang SX,Pei JJ

    更新日期:2012-04-01 00:00:00

  • EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells.

    abstract::1. Hypothalamic magnocellular neurons synthesize, store, and secrete large quantities of the neuropeptides, vasopressin (VP) and oxytocin (OT), which are synthesized as protein precursors also containing proteins called neurophysins. These protein precursors are sorted through the regulated secretory pathway (RSP), pa...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zhang BJ,Yamashita M,Fields R,Kusano K,Gainer H

    更新日期:2005-06-01 00:00:00

  • A synergic role of caspase-6 and caspase-3 in Tau truncation at D421 induced by H2O 2.

    abstract::Tau truncation is widely detected in Alzheimer's disease brain. Caspases activation is suggested to play a significant role in tau truncation at Aspartate 421 (D421) according to their ability to cleave recombinant tau in vitro. Ample evidence has shown that caspase-6 is involved in cognitive impairment and expressed ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zhao H,Zhao W,Lok K,Wang Z,Yin M

    更新日期:2014-04-01 00:00:00

  • Neuronal membrane depolarization and the control of cholinergic muscarinic receptors: selective effect on different neuronal cell types.

    abstract::1. The possibility that a long-lasting neuronal activation regulates the expression of muscarinic cholinergic receptors was studied with three cultured neuronal cell lines. 2. Continuous depolarization of a subclone of the neuroblastoma-glioma NG108-15 hybrid cells with potassium chloride increased by 45-75% the numbe...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Simantov R,Levy R

    更新日期:1989-03-01 00:00:00

  • Kinetics of acetylcholine-activated cation channel blockade by the calcium antagonist D-600 in Aplysia neurons.

    abstract::The effects of the calcium channel blocker D-600 on the cation channels activated by acetylcholine (ACh) was studied in voltage-clamped Aplysia neurons by voltage-jump relaxation analysis. D-600 blocked the steady-state ACh current in a highly voltage-dependent manner, the degree of antagonism increasing with membrane...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Slater NT,Haas HL,Carpenter DO

    更新日期:1983-12-01 00:00:00

  • Differential PARP cleavage: an indication of heterogeneous forms of cell death and involvement of multiple proteases in the infarct of focal cerebral ischemia in rat.

    abstract:AIM:Poly (ADP-ribose) polymerase (PARP) is a nuclear repair enzyme whose role is widely depicted in various physiological and pathological processes. In the present study, we wanted to check the status of PARP and the role of various cell death proteases involved in apoptotic and non-apoptotic forms of cell death durin...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Chaitanya GV,Babu PP

    更新日期:2009-06-01 00:00:00

  • Block by 5-hydroxytryptamine of neuronal acetylcholine receptor channels expressed in Xenopus oocytes.

    abstract::1. Effects of 5-hydroxytryptamine (5-HT) on neuronal nicotinic acetylcholine (ACh) receptor channels were investigated by expressing cloned channel subunits in Xenopus oocytes. 2. When channels were expressed with a combination of alpha 3 and beta 4 subunits, 5-HT (10 to 300 microM) reversibly inhibited an inward curr...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Nakazawa K,Akiyama T,Inoue K

    更新日期:1995-08-01 00:00:00

  • Down-regulated GABAergic expression in the olfactory bulb layers of the mouse deficient in monoamine oxidase B and administered with amphetamine.

    abstract::This study explores primarily the role of the activity of monoamine oxidase B (MAOB) in the regulation of glutamic acid decarboxylase(67) (GAD(67)) expression in distinct layers of main olfactory bulb (OlfB), which links the limbic system. Moreover, the response of GAD(67) was investigated to amphetamine perturbation ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Yin HS,Chen K,Shih JC,Tien TW

    更新日期:2010-05-01 00:00:00

  • Dynamic change of Prohibitin2 expression in rat sciatic nerve after crush.

    abstract::As a novel cell cycle inhibitor, PHB2 controls the G1/S transition in cycling cells in a complex manner. Its aberrant expression is closely related to cell carcinogenesis. While its expression and role in peripheral nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve crush (S...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Long L,Huang Y,Wu H,Luan W,Zhang Q,Wen H,Ding T,Wang Y

    更新日期:2013-07-01 00:00:00

  • Hck Promotes Neuronal Apoptosis Following Intracerebral Hemorrhage.

    abstract::The hematopoietic cell kinase (Hck) is a member of the Src family protein kinases which regulates many signal transduction pathways including cell growth, proliferation, differentiation, migration, and apoptosis. However, the expression and function of Hck after intracerebral hemorrhage (ICH) are unknown. Western blot...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Wang J,Chen R,Liu X,Shen J,Yan Y,Gao Y,Tao T,Shi J

    更新日期:2017-03-01 00:00:00

  • Characterization of NO/cGMP-mediated responses in identified motoneurons.

    abstract::1. Nitric oxide (NO) is thought to play a neuromodulatory role in the nervous system of vertebrate and invertebrate species. In the hornworm Manduca sexta, NO-mediated signaling has been implicated in behavioral and developmental processes, but its exact function in neurons is unknown. In this study, we identify speci...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zayas RM,Trimmer BA

    更新日期:2007-03-01 00:00:00

  • Combination of dexamethasone and aminoguanidine reduces secondary damage in compression spinal cord injury.

    abstract::The study was performed to investigate the effect of combination therapy with aminoguanidine (AG) and dexamethasone (DEX) on the compression spinal cord injury (SCI) in rat. Compared to the control group, the combination therapy group with AG (75 mg/kg) and DEX (0.025 mg/kg) significantly reduced the degree of (1) spi...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Xu WB,Lv G,Wang YF,Lu XH,Huang T,Zhu Y,Jia LS

    更新日期:2009-07-01 00:00:00

  • Divalent cation effects on acetylcholine-activated channels at the frog neuromuscular junction.

    abstract::The effects of the divalent cations Ca and Mg on the properties of ACh-activated channels at the frog neuromuscular junction were studied using a two-microelectrode voltage clamp. The divalent cation concentration was varied from 2 to 40 mM in solutions containing 50% normal Na. The reversal potential was determined b...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Lewis CA

    更新日期:1984-09-01 00:00:00

  • Function of Cathepsin K in the Central Nervous System of Male Mice is Independent of Its Role in the Thyroid Gland.

    abstract::Cathepsin K deficiency in male mice (Ctsk-/-) results in decreased numbers of hippocampal astrocytes and altered neuronal patterning as well as learning and memory deficits. Additionally, cathepsin K carries essential roles in the thyroid gland where it contributes to the liberation of thyroid hormones (TH). Because T...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Dauth S,Rakov H,Sîrbulescu RF,Ilieş I,Weber J,Batbajar Dugershaw B,Braun D,Rehders M,Wirth EK,Führer D,Schweizer U,Brix K

    更新日期:2020-07-01 00:00:00

  • Up-regulation of podoplanin involves in neuronal apoptosis in LPS-induced neuroinflammation.

    abstract::Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Song Y,Shen J,Lin Y,Shen J,Wu X,Yan Y,Zhou L,Zhang H,Zhou Y,Cao M,Liu Y

    更新日期:2014-08-01 00:00:00

  • Past Exposure to Cigarette Smoke Aggravates Cognitive Impairment in a Rat Model of Vascular Dementia via Neuroinflammation.

    abstract::Smoking is a risk factor for dementia. Cognitive function can be partially restored after quitting smoking, but still lower than never smoked group. The underlying mechanisms still remain unclear. The effects of smoking cessation combined with cerebral chronic hypoperfusion (CCH) on cognitive function have never been ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Meng N,Dong Y,Huo T,Song M,Jiang X,Xiao Y,Lv P

    更新日期:2020-11-06 00:00:00

  • DNA immunization against amyloid beta 42 has high potential as safe therapy for Alzheimer's disease as it diminishes antigen-specific Th1 and Th17 cell proliferation.

    abstract::The pathogenesis of Alzheimer's disease (AD) has been strongly associated with the accumulation of amyloid beta (Aβ) peptides in brain, and immunotherapy targeting Aβ provides potential for AD prevention. A clinical trial in which AD patients were immunized with Aβ42 peptide was stopped when 6% of participants showed ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Lambracht-Washington D,Qu BX,Fu M,Anderson LD Jr,Stüve O,Eagar TN,Rosenberg RN

    更新日期:2011-08-01 00:00:00

  • Opioid effects in developing rat vas deferens.

    abstract::The inhibitory effects of various opiates on developing rat vas deferens were studied by determining the degree of depression of mechanical responses elicited by electrical field stimulation. All agonists showed decreased effects with maturation, but the decrease occurred at different times. With normorphine the loss ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Rónai AZ,Berzétei IP,Kurgyis J

    更新日期:1981-12-01 00:00:00

  • Neuroprotective potential of fasudil mesylate in brain ischemia-reperfusion injury of rats.

    abstract::We previously reported that inhibition of Rho-kinase (ROCK) by hydroxyl fasudil improves cognitive deficit and neuronal damage in rats with chronic cerebral ischemia (Huang et al., Cell Mol Neurobiol 28:757-768, 2008). In this study, fasudil mesylate (FM) was investigated for its neuroprotective potential in rats with...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Li Q,Huang XJ,He W,Ding J,Jia JT,Fu G,Wang HX,Guo LJ

    更新日期:2009-03-01 00:00:00

  • miR-9 Upregulation Integrates Post-ischemic Neuronal Survival and Regeneration In Vitro.

    abstract::The irrefutable change in the expression of brain-enriched microRNAs (miRNAs) following ischemic stroke has promoted the development of radical miRNA-based therapeutics encompassing neuroprotection and neuronal restoration. Our previous report on the systems-level prediction of miR-9 in post-stroke-induced neurogenesi...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Nampoothiri SS,Rajanikant GK

    更新日期:2019-03-01 00:00:00

  • Comparison of trophic factors changes in the hippocampal CA1 region between the young and adult gerbil induced by transient cerebral ischemia.

    abstract::In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Yan BC,Park JH,Kim SK,Choi JH,Lee CH,Yoo KY,Kwon YG,Kim YM,Kim JD,Won MH

    更新日期:2012-11-01 00:00:00

  • The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice.

    abstract::1. Wobbler mice suffer an autosomal recessive mutation producing severe motoneuron degeneration and dense astrogliosis, with increased levels of glial fibrillary acidic protein (GFAP) in the spinal cord and brain stem. They have been considered animal models of amyotrophic lateral sclerosis and infantile spinal muscul...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Gonzalez Deniselle MC,Gonzalez SL,Piroli GG,Lima AE,De Nicola AF

    更新日期:1996-02-01 00:00:00

  • Glutamate transmission in the rostral ventrolateral medullary sympathetic premotor pathway.

    abstract::1. The aim of these studies was to test the hypothesis that glutamate is the principal excitatory neurotransmitter in the sympathetic premotor pathway from the rostral ventrolateral medulla (RVLM) to the sympathetic preganglionic neurons (SPNs) in the thoracic spinal cord. 2. Iontophoretic and pressure ejection of glu...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Morrison SF

    更新日期:2003-10-01 00:00:00