Abstract:
:1. Differential regulation, by dexamethasone, of glucocorticoid receptor gene expression was studied in three different neuronal cultures derived from hypothalamus amygdala, and cerebral cortex. 2. Cellular glucocorticoid receptor (GR) mRNA concentration was measured by hybridization using a 32P-labeled RNA probe complementary to a 2.2-kb fragment of the glucocorticoid receptor mRNA. Changes in the amount of GR mRNA were evaluated in relation to the content of beta-actin mRNA. 3. In cells derived from either hypothalamus or cerebral cortex, we observed a complex pattern of GR mRNA concentrations which were characterized by cyclic variations of GR mRNA content during continuous treatment with dexamethasone for up to 72 hr. 4. In contrast to cells derived from the hypothalamus where a persistent 30-40% reduction in GR mRNA levels was seen for up to a least 72 hr, we observed, in cells derived from the cerebral cortex, a sustained increased (1.4-fold) of the GR mRNA at this same time interval.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Pepin MC,Beaulieu S,Barden Ndoi
10.1007/BF00734576subject
Has Abstractpub_date
1990-06-01 00:00:00pages
227-35issue
2eissn
0272-4340issn
1573-6830journal_volume
10pub_type
杂志文章abstract::1. The aims of the present study were (a) to determine the identity of the G proteins with which the endothelin receptor interacts and whether this interaction is subtype specific and (b) to determine whether agonist exposure can result in specific coupling between the endothelin receptor and G proteins. 2. Coupling b...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1007010125316
更新日期:2000-06-01 00:00:00
abstract::1. Aminoalkylindoles, typified by WIN 55212-2, bind to G protein-coupled cannabinoid receptors in brain. Although cannabinoids inhibit adenylyl cyclase in NG108-15 neuroblastoma x glioma hybrid cells, cannabinoid receptor binding in these cells has not been described previously. This study compares pharmacological cha...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1026306804802
更新日期:1997-10-01 00:00:00
abstract:AIM:In Alzheimer's disease (AD) it is well known that specific regions of the brain are particularly vulnerable to the pathologic insults of the disease. In particular, the hippocampus is affected very early in the disease and by end stage AD is ravaged by neurofibrillary tangles and senile plaques (i.e., the pathologi...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1025063811290
更新日期:2003-10-01 00:00:00
abstract::1. Sporadic Creutzfeldt-Jakob disease (CJD) is a rapidly progressive and fatal disease. Patients with CJD usually become akinetic mutism within approximately 6 months. In addition, clinical signs and symptoms at early stage of sporadic CJD may not be easy to distinguish from other neurodegenerative diseases by neurolo...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-006-9370-z
更新日期:2006-02-01 00:00:00
abstract:AIM:A fraction of vesicles in cells treated with hypertonic solution exhibit multiple dense cores and this is enhanced by treatment with L-3,4-dihydroxyphenylalanine (L-DOPA). These cells were examined to determine if the multicore vesicles are the product of endocytosis or homotypic fusion. METHODS:Electron microscop...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-007-9156-y
更新日期:2007-08-01 00:00:00
abstract::1. Spatio-temporal changes in phosphorylated (pNFP) and nonphosphorylated (npNFP) neurofilament proteins were assessed immunocytochemicaly in adult rat cerebellum, 2-30 days following unilateral injection of kainic acid (KA) or physiological saline (s.c.). 2. Analysis of the staining intensity and pattern demonstrated...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/b:cemn.0000022769.44211.2b
更新日期:2004-06-01 00:00:00
abstract::Traumatic brain injury (TBI) is a leading cause of death and long-term disability. Fucoidan, a sulfated polysaccharide extracted from brown algae, possesses potent anti-oxidative and anti-inflammatory effects. Considering TBI happens frequently in adults, especially in aged individuals, we herein sought to define the ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-015-0323-2
更新日期:2016-11-01 00:00:00
abstract::The prelimbic cortex (PL) is an important structure in the neural pathway integrating stress responses. Brain angiotensin is involved in cardiovascular control and modulation of stress responses. Blockade of angiotensin receptors has been reported to reduce stress responses. Acute restraint stress (ARS) is a stress mo...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-017-0518-9
更新日期:2018-01-01 00:00:00
abstract::1. Transient forebrain ischemia in adult rats, induced by 10 min of bilateral carotid occlusion and an arterial hypotension of 40 mmHg, caused substantial damage not only to CA-1 neurons in hippocampus but also to epithelial cells in lateral ventricle choroid plexus. 2. When transient forebrain ischemia was followed b...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1007097622590
更新日期:2000-04-01 00:00:00
abstract::Organotellurium compounds have been synthesized since 1840, but pharmacological and toxicological studies about them are still incipient. Therefore, the objective of this study was to verify the effect of acute administration of the organochalcogen 3-butyl-1-phenyl-2-(phenyltelluro)oct-en-1-one on some parameters of o...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9547-3
更新日期:2010-10-01 00:00:00
abstract::1. 3H-gamma-Aminobutyric acid (GABA) release elicited by a depolarizing K+ stimulus or by noradrenergic transmitter was examined in rat pineals in vitro. 2. The release of 3H-GABA was detectable at a 20 mM K+ concentration in medium and increased steadily up to 80 mM K+. 3. In a Ca2+-free medium 3H-GABA release elicit...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00713029
更新日期:1989-06-01 00:00:00
abstract::1. RNA interference (RNAi) is a multicomponent machinery that operates in a sequence-specific manner to repress the expression of genes in most eukaryotic cells. 2. Here we wanted to investigate in a murine neuroblastoma cell line (NBP2) (a) if replacement of the loop of the short hairpin RNA (shRNA) with a hammerhead...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-004-6919-6
更新日期:2004-12-01 00:00:00
abstract::microRNAs (miRNAs) comprise a class of ~18-25 nucleotide (nt) single-stranded non-coding RNAs (sncRNAs) that are the smallest known carriers of gene-encoded, post-transcriptional regulatory information in both plants and animals. There are many fundamental similarities between plant and animal miRNAs-the miRNAs of bot...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-017-0547-4
更新日期:2018-01-01 00:00:00
abstract::Repeated electrical stimulation results in development of seizures and a permanent increase in seizure susceptibility (kindling). The permanence of kindling suggests that chronic changes in gene expression are involved. Kindling at different sites produces specific effects on interictal behaviors such as spatial cogni...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-011-9672-7
更新日期:2011-08-01 00:00:00
abstract::1. The possibility that a long-lasting neuronal activation regulates the expression of muscarinic cholinergic receptors was studied with three cultured neuronal cell lines. 2. Continuous depolarization of a subclone of the neuroblastoma-glioma NG108-15 hybrid cells with potassium chloride increased by 45-75% the numbe...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711445
更新日期:1989-03-01 00:00:00
abstract::Neuronal degeneration and astrogliosis are hallmarks of prion disease. Synthetic prion protein (PrP) peptide 106-126 (PrP106-126) can induce death of neurons and proliferation of astrocytes in vitro and this neurotoxic effect depends on the expression of cellular PrP (PrPC) and is hence believed to be PrP(C) -mediated...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-005-8357-5
更新日期:2005-12-01 00:00:00
abstract::Smoking is a risk factor for dementia. Cognitive function can be partially restored after quitting smoking, but still lower than never smoked group. The underlying mechanisms still remain unclear. The effects of smoking cessation combined with cerebral chronic hypoperfusion (CCH) on cognitive function have never been ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-020-00992-2
更新日期:2020-11-06 00:00:00
abstract::Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of Arctium lappa L. roots (EAL) on hydrogen peroxide (H2O...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0129-7
更新日期:2015-04-01 00:00:00
abstract::The mode of action of the cholinergic antagonist hexamethonium on the excitatory responses of voltage-clamped Aplysia neurons to acetylcholine (ACh) has been examined by voltage- and concentration-jump relaxation analysis. At steady-state concentrations of ACh hyperpolarizing command steps induced inward current relax...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00711070
更新日期:1986-06-01 00:00:00
abstract::Oligodendrocyte progenitor cell (OPC) migration is critical for effective myelination of the central nervous system. Not only during normal myelination but also during remyelination, the growth factors (GFs) and extracellular matrix (ECM) protein affect the OPC migration. Studies showed the altered levels of GFs and E...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-016-0359-y
更新日期:2017-03-01 00:00:00
abstract::Normokalemic periodic paralysis (normoPP) is a type of skeletal muscle function disorder which is characterized by paralysis attack with concomitant normal serum potassium level. We previously reported that R675Q mutation of human skeletal muscle voltage-gated sodium channel α subunit (SCN4A) may be the novel mutation...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0052-y
更新日期:2014-07-01 00:00:00
abstract::We have previously demonstrated that alpha2-adrenoceptors regulate hypothalamic magnocellular oxytocinergic (OXY) neurons in Sprague Dawley rats. Here we investigated whether activation/inhibition of alpha2-adrenoceptors may similarly trigger/downregulate the activity of OXY neurons in control Long Evans (+/+) and per...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9388-0
更新日期:2009-09-01 00:00:00
abstract::To clarify the role of angiotensin II (Ang II) in the regulation of sensory signaling, we studied the effect of subpressor dose (150 ng/kg/min) of Ang II on pain-related behavior in relation with neuronal injury and activation of satellite glial cells (SGCs) in the dorsal root ganglia (DRGs) after chronic constriction...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9934-7
更新日期:2013-07-01 00:00:00
abstract::The insulin-like growth factor (IGF) system is linked to CNS pathological states. The functions of IGFs are modulated by a family of binding proteins termed insulin-like growth factor binding proteins (IGFBPs). Here, we demonstrate that IGFBP-6 may be associated with neuronal apoptosis in the processes of intracerebra...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-016-0453-1
更新日期:2017-10-01 00:00:00
abstract::Amyotrophic lateral sclerosis is a devastating motoneuron disorder for which no effective treatment exists. There is some evidence for neuroprotective effects of valproic acid (VPA). The beneficial effects, however, are limited due to the adverse effects of VPA. To overcome this problem, a number of VPA derivates with...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9518-8
更新日期:2010-08-01 00:00:00
abstract::1. The electrophysiological actions of excitatory amino acids and serotonin were investigated in slices from cat neocortex in vitro. Intracellular recordings were obtained from neurons (mainly in layer V) and the drugs applied extracellularly to the same neurons by microiontophoresis. 2. Serotonin, and to some extent ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00733789
更新日期:1987-12-01 00:00:00
abstract::(1) There is increasing evidence that the cerebral endothelium and the blood-brain barrier (BBB) plays an important role in the oxidative stress-induced brain damage. The aim of the present study was to investigate the role of interendothelial junctional proteins in the BBB permeability increase induced by oxidative s...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-004-1378-7
更新日期:2005-02-01 00:00:00
abstract::1. The retinoblastoma susceptibility gene product, p105Rb (RB), is an important regulator in the control of cell proliferation, differentiation, and apoptosis. Several cellular factors that complex with RB and exert their cellular regulatory functions have been identified, such as the RB:cyclophilin A (CypA) complex. ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/b:cemn.0000005321.11544.cc
更新日期:2003-12-01 00:00:00
abstract::The hypothesis that peripheral, skeletal muscle tissue contains a trophic factor supporting central neurons has recently been investigated in vitro by supplementing the culture medium of spinal cord neurons with muscle extracts and fractions of extract. We extended these studies asking whether or not a trophic factor ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00710943
更新日期:1984-03-01 00:00:00
abstract::1. Gamma-aminobutryic acid (GABA), a major inhibitory transmitter of the vertebrate retina, is synthesized from glutamate by L-glutamate decarboxylase (GAD) and mediates neuronal inhibition at GABAA receptors. GAD consists of two distinct molecular forms, GAD65 and GAD67, which have similar distribution patterns in th...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00734812
更新日期:1991-10-01 00:00:00