Phenotyping of pig alpha 1B-glycoprotein (PO2) and haemopexin by 1D polyacrylamide gel electrophoresis and immunoblotting.

Abstract:

:Described is an alternative procedure for the phenotyping of pig alpha 1B-glycoprotein (PO2) and haemopexin. The procedure is based on the separation of serum samples by horizontal polyacrylamide gel electrophoresis, passive blotting onto a nitrocellulose (NC) sheet, and immunochemical detection using a mixture of a primary antibody (rabbit anti-pig alpha 1B or anti-pig haemopexin) and a peroxidase-labelled secondary antibody. Several NC copies can be obtained from a single gel and these can be developed with different monospecific antisera.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Kaláb P,Stratil A

doi

10.1111/j.1365-2052.1989.tb00870.x

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

295-8

issue

3

eissn

0268-9146

issn

1365-2052

journal_volume

20

pub_type

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