Abstract：
:A vaccine that induces broadly neutralizing antibodies (bnAbs) against the human immunodeficiency virus (HIV) would be instrumental in controlling the disease. The membrane proximal external region (MPER) peptide is an appealing antigen candidate since it is conserved and is the target of several human bnAbs, such as 2F5. We previously found that liposomes containing cobalt porphyrin-phospholipid (CoPoP) can bind to a his-tagged MPER peptide, resulting in biomimetic antigen presentation on a lipid bilayer. The present study generated various his-tagged, synthetic MPER fragments, which were bound to liposomes containing CoPoP and a synthetic monophosphoryl lipid A (MPLA) and assessed for immunogenicity in mice. MPER peptides with amino acids stretches originating from the membrane insertion point that were at least 25 amino acids in length, had greater 2F5 reactivity and induced stronger antibody responses, compared to shorter ones. Immunization with the lipid-presented MPER elicited stronger antibody responses compared to Alum and Montanide adjuvants, which could recognize recombinant gp41 and gp140 proteins that contained MPER sequences. The induced antibodies neutralized a tier 1A virus that is sensitive to neutralizing antibodies (W61D(TCLA)0.71), but not another tier 1A nor a tier 2 strain. Co-formulation of the MPER peptide with an unrelated malaria protein antigen (Pfs25) that is effectively adjuvanted with liposomes containing CoPoP and MPLA resulted in elicitation of higher MPER antibody levels, but did not improve neutralization, possibly due to interference with proper peptide presentation in the membrane. Murine hybridomas were generated that produced MPER antibodies, but they were non-neutralizing. These results do not show that bnAbs could be generated with MPER peptides and CoPoP liposomes, but do not rule out this possibility with additional improvements to the approach.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Shao S,Huang WC,Lin C,Hicar MD,LaBranche CC,Montefiori DC,Lovell JFdoi
10.1007/s10439-019-02398-8subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1991-2001issue
7eissn
0090-6964issn
1573-9686pii
10.1007/s10439-019-02398-8journal_volume
48pub_type
杂志文章abstract：:Despite cellular environments having dynamic characteristics, many laboratories utilized static polyacrylamide hydrogels to study the ECM-cell relationship. To attain a more in vivo like environment, we have developed a dynamic, DNA-crosslinked hydrogel (DNA gel). Through the controlled delivery of DNA, we can tempora...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0483-2
更新日期：2012-05-01 00:00:00
abstract：:Traditionally, measurement of pulmonary O2 uptake uses mass balance of N2 to correct for differences between inspired and expired volume (V) due to temperature (T) and relative humidity (RH). Often during anesthesia, N2 balance cannot be invoked due to high inspired O2 fraction (FIO2) or nonsteady state conditions. Th...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1312184
更新日期：2000-09-01 00:00:00
abstract：:Opportunities to provide clinical immersion experiences to bioengineering undergraduate students have expanded over the last several years. These programs allow students to observe the clinical environment in order to better understand workflow processes, the context in which medical equipment is used, and identify un...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02508-x
更新日期：2020-09-01 00:00:00
abstract：:This paper presents the permeability analysis of neuroactive drugs and correlation with in vivo brain/plasma ratios in a dynamic microfluidic blood-brain barrier (BBB) model. Permeability of seven neuroactive drugs (Ethosuximide, Gabapentin, Sertraline, Sunitinib, Traxoprodil, Varenicline, PF-304014) and trans-endothe...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1086-5
更新日期：2014-12-01 00:00:00
abstract：:We examine the low-frequency limit of hearing of the mammalian ear through the analytical modelling of the natural frequency of the tympanic membrane. The resulting equation of the natural frequency of the modelled tympanic membrane is numerically verified against previous theoretical studies, and is statistically val...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02356-4
更新日期：2020-01-01 00:00:00
abstract：:Computational modeling and simulation has become more common in design and development of bioprosthetic heart valves. To have a reliable computational model, considering accurate mechanical properties of biological soft tissue is one of the most important steps. The goal of this study was to present a non-invasive mat...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02129-5
更新日期：2019-01-01 00:00:00
abstract：:The compression of blood vessels by surrounding tissue is an important problem in hemodynamics, most prominently in studies relating to the heart. In this study we consider a long tube of elliptic cross section as an idealization of the geometry of a compressed blood vessel. An exact solution of the governing equation...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.106
更新日期：1998-09-01 00:00:00
abstract：:There is a significant clinical need for engineered bone graft substitutes that can quickly, effectively, and safely repair large segmental bone defects. One emerging field of interest involves the growth of engineered bone tissue in vitro within bioreactors, the most promising of which are perfusion bioreactors. Usin...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1662-y
更新日期：2016-12-01 00:00:00
abstract：:The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field (SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the changes in alkaline phosphatase activity and electron microscopy images. Me...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9370-2
更新日期：2007-11-01 00:00:00
abstract：:Elastin is an essential component of arteries which provides structural integrity and instructs smooth muscle cells to adopt a quiescent state. Despite interaction of endothelial cells with elastin in the internal elastic lamina, the potential for exploiting this interaction therapeutically has not been explored in de...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0142-z
更新日期：2011-01-01 00:00:00
abstract：:It is proposed that the maximum in cuff pressure oscillations during oscillometry is due to the buckling of the brachial artery under a cuff. This theory is investigated by means of a mathematical model of oscillatometry that includes the mechanics of the occlusive arm cuff, the arterial pressure pulse waveform, and t...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368225
更新日期：1994-01-01 00:00:00
abstract：:High energy laser has emerged to be an important surgical tool in medical technology. However, the application of laser energy to drill or cut a bone is still in an experimental state. In order to estimate the adequacy of laser heating of bone surface and at the same time minimize the damage to the bone tissue, we dev...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02363565
更新日期：1987-01-01 00:00:00
abstract：:The pericellular matrix (PCM) is a region of tissue that surrounds chondrocytes in articular cartilage and together with the enclosed cells is termed the chondron. Previous studies suggest that the mechanical properties of the PCM, relative to those of the chondrocyte and the extracellular matrix (ECM), may significan...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-4479-7
更新日期：2005-10-01 00:00:00
abstract：:To study the effects of compressive straining on muscle cell deformation and damage an in vitro model system was developed. Myoblasts were seeded in agarose constructs and cultured in growth medium for 4 days. Subsequently, the cells were allowed to fuse into multinucleated myotubes for 8 days in differentiation mediu...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1349698
更新日期：2001-02-01 00:00:00
abstract：:Femoral neck fractures are a relevant clinical and social problem. The aim of this study was to improve the prediction of patients at-risk of femoral neck fracture with respect to the current densitometric-based methods. In particular, finite element models were used to assess the prediction accuracy obtained by combi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1495866
更新日期：2002-06-01 00:00:00
abstract：:In physiological conditions, joint function involves continuously moving contact areas over the tissue surface. Such moving contacts play an important role for the durability of the tissue. It is known that in pathological joints these motion paths and contact mechanics change. Nevertheless, limited information exists...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1543-9
更新日期：2016-08-01 00:00:00
abstract：:The pulmonary microvasculature offers a heterogeneous barrier to the motion of large solutes as they pass between blood and lymph. While this barrier has been approximated by a few discrete pathways or by statistical ensembles of many pathways, these descriptions only partly capture the structural and functional prope...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368425
更新日期：1990-01-01 00:00:00
abstract：:Cochlear implantation consists in electrically stimulating the auditory nerve by inserting an electrode array inside the cochlea, a bony structure of the inner ear. In the absence of any visual feedback, the insertion results in many cases of damages of the internal structures. This paper presents a feasibility study ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02671-1
更新日期：2020-10-30 00:00:00
abstract：:Abnormal scapular movement is widely believed to be an important factor in clinical pathology of the shoulder joint complex. Validated non-invasive techniques for measuring scapular movement have been developed, but the effect of marker placement on accuracy is unknown. The objective of this study was to determine the...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0185-1
更新日期：2011-02-01 00:00:00
abstract：:In this study, we have developed an integrated microfluidic platform for actively patterning mammalian cells, where poly(ethylene glycol) (PEG) hydrogels play two important roles as a non-fouling layer and a dielectric structure. The developed system has an embedded array of PEG microwells fabricated on a planar indiu...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0108-1
更新日期：2010-12-01 00:00:00
abstract：:Microstructural characterization of cardiac tissue and its remodeling in disease is a crucial step in many basic research projects. We present a comprehensive approach for three-dimensional characterization of cardiac tissue at the submicrometer scale. We developed a compression-free mounting method as well as labelin...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1465-6
更新日期：2016-05-01 00:00:00
abstract：:Deep tissue injury (DTI) is a type of pressure ulcer in which tissue breakdown initiates at the bone-muscle interface under intact skin. Excessive deformation in the soft tissue, particularly around bony prominences, is believed to be one of the causes leading to the development of DTI. The main goal of this study was...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0539-y
更新日期：2012-08-01 00:00:00
abstract：:Spine musculoskeletal models used to estimate loads and displacements require many simplifying assumptions. We examined how assumptions about subject size and vertebral positions can affect the model outcomes. Head and neck models were developed to represent 30 subjects (15 males and 15 females) in neutral posture and...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-018-2084-9
更新日期：2018-11-01 00:00:00
abstract：:Lung cancer is a leading cause of death worldwide. Radiation therapy (RT) is one method to treat this disease. A common side effect of RT for lung cancer is radiation-induced lung damage (RILD) which leads to loss of lung function. RILD often compounds pre-existing smoking-related regional lung function impairment. It...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02697-5
更新日期：2020-11-30 00:00:00
abstract：:The most widely implanted prosthetic valve is the mechanical bileaflet. Recent clinical experiences suggest that some designs are more prone to thromboembolic episodes than others. This study evaluated the hinge flow and near-field forward flow of the new St. Jude Medical Regent bileaflet mechanical heart valve. Laser...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.297
更新日期：2000-05-01 00:00:00
abstract：:Recent advances in angioplasty have involved the application of polymer coatings to stent surfaces for purposes of drug delivery. Given the high levels of deformation developed in the plastic hinge of a stent during deployment, the achievement of an intact bond between the coating and the stent presents a significant ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9972-y
更新日期：2010-07-01 00:00:00
abstract：:Articular cartilage is an avascular connective tissue responsible for bearing loads. Cell signaling plays a central role in cartilage homeostasis and tissue engineering by directing chondrocytes to synthesize/degrade the extracellular matrix or promote inflammatory responses. The aim of this paper was to investigate a...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1964-8
更新日期：2018-02-01 00:00:00
abstract：:A novel electrocardiogram (ECG) electrode film is developed by mixing carbon black powder and a quaternary salt with a visco-elastic polymeric adhesive. Unlike traditional wet gel-based electrodes, carbon/salt/adhesive (CSA) electrodes should theoretically have an infinite shelf life as they do not dehydrate even afte...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1282-y
更新日期：2015-10-01 00:00:00
abstract：:Instrumentation systems for breath-to-breath analysis of respiratory gas exchange have been faulted by phase lags between various flow and composition signals and by difficulties in gathering and processing large amounts of data. The system described here represents an attempt to overcome these problems. Phase delays ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02367493
更新日期：1983-01-01 00:00:00
abstract：:This paper reports the design, manufacturing, and characterization of a new bioreactor dedicated to the development of tissue-engineered heart valve substitutes. First, a comprehensive review of the state of the art in bioreactors is presented and a rigorous classification is put forward. The existing bioreactors foun...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9646-9
更新日期：2009-04-01 00:00:00
