3D Printed Vascular Networks Enhance Viability in High-Volume Perfusion Bioreactor.

Abstract:

:There is a significant clinical need for engineered bone graft substitutes that can quickly, effectively, and safely repair large segmental bone defects. One emerging field of interest involves the growth of engineered bone tissue in vitro within bioreactors, the most promising of which are perfusion bioreactors. Using bioreactor systems, tissue engineered bone constructs can be fabricated in vitro. However, these engineered constructs lack inherent vasculature and once implanted, quickly develop a necrotic core, where no nutrient exchange occurs. Here, we utilized COMSOL modeling to predict oxygen diffusion gradients throughout aggregated alginate constructs, which allowed for the computer-aided design of printable vascular networks, compatible with any large tissue engineered construct cultured in a perfusion bioreactor. We investigated the effect of 3D printed macroscale vascular networks with various porosities on the viability of human mesenchymal stem cells in vitro, using both gas-permeable, and non-gas permeable bioreactor growth chamber walls. Through the use of 3D printed vascular structures in conjunction with a tubular perfusion system bioreactor, cell viability was found to increase by as much as 50% in the core of these constructs, with in silico modeling predicting construct viability at steady state.

journal_name

Ann Biomed Eng

authors

Ball O,Nguyen BB,Placone JK,Fisher JP

doi

10.1007/s10439-016-1662-y

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

3435-3445

issue

12

eissn

0090-6964

issn

1573-9686

pii

10.1007/s10439-016-1662-y

journal_volume

44

pub_type

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