Abstract：
:Chinese hamster ovary (CHO) cells are the main workhorse in the biopharmaceutical industry for the production of recombinant proteins, such as monoclonal antibodies. To date, a variety of metabolic engineering approaches have been used to improve the productivity of CHO cells. While genetic manipulations are potentially laborious in mammalian cells, rational design of CHO cell culture medium or efficient fed-batch strategies are more popular approaches for bioprocess optimization. In this study, a genome-scale metabolic network model of CHO cells was used to design feeding strategies for CHO cells to improve monoclonal antibody (mAb) production. A number of metabolites, including threonine and arachidonate, were suggested by the model to be added into cell culture medium. The designed composition has been experimentally validated, and then optimized, using design of experiment methods. About a two-fold increase in the total mAb expression has been observed using this strategy. Our approach can be used in similar bioprocess optimization problems, to suggest new ways of increasing production in different cell factories.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Fouladiha H,Marashi SA,Torkashvand F,Mahboudi F,Lewis NE,Vaziri Bdoi
10.1007/s00449-020-02332-6subject
Has Abstractpub_date
2020-08-01 00:00:00pages
1381-1389issue
8eissn
1615-7591issn
1615-7605pii
10.1007/s00449-020-02332-6journal_volume
43pub_type
杂志文章abstract：:Food safety is a global health and socioeconomic concern since many people still suffer from various acute and life-long diseases, which are caused by consumption of unsafe food. Therefore, ensuring safety of the food is one of the most essential issues in the food industry, which needs to be considered during not onl...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-019-02256-w
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
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更新日期：2008-06-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
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更新日期：2011-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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journal_title：Bioprocess and biosystems engineering
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更新日期：2012-01-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0951-1
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journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
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abstract：:A two-phase dispersion technique, termed emulsification-internal gelation, is proposed for encapsulation of activated sludge in gellan gum microbeads. The influence of emulsion parameters on size distribution of microbeads was investigated. Mean diameter of microbeads varied within a range of 34-265 microm as a descen...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-004-0360-6
更新日期：2004-07-01 00:00:00
