Abstract:
:The influence of sugars in culture media on the kinetics of the mono- and disaccharide uptake and cell growth behavior was studied by mid-infrared spectroscopy using a Fourier transform infrared spectrometer (FT-IR) equipped with an attenuate total reflection accessory (ATR). We performed the plant cell cultivation with Nicotiana tabacum cv. Bright Yellow No.2 (TBY-2) cells in the culture media, which contained glucose, fructose, mannose, galactose, sucrose, trehalose, maltose or lactose. Consequently, the differences of the kinetic sugar uptake and cell growth behavior among all the cultivations were confirmed. In particular, a very long lag time before the galactose uptake was observed, and the spectral-pattern of the maltose medium presented almost the same as the initial one during the cultivation. Furthermore, base on the non-dimensional cultivation time for cell growth behavior, it was suggested that the TBY-2 cells consumed sugar before cell growth and produced the ethanol just after cell growth.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Yamanaka A,Hashimoto A,Matsuo T,Kanou M,Suehara K,Kameoka Tdoi
10.1007/s00449-007-0142-zsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
457-68issue
6eissn
1615-7591issn
1615-7605journal_volume
30pub_type
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