Abstract：
:The performance and stability of a two-phase partitioning bioscrubber (TPPB) containing 33% (vol.) n-hexadecane as an immiscible phase was investigated during 30 days of continuous gaseous benzene treatment. Elimination capacities of 141 +/- 12 g/m(3) h were achieved by Achromobacter xylosoxidans Y234 while maintaining >99% removal throughout. A new steady-state operating strategy that limits excessive biomass production by directing substrate consumption to maintenance energy has eliminated the requirement for frequent exchange of liquid contents. Simplifying the operating protocols in this manner has dramatically reduced material costs and rendered the TPPB operational requirements as more comparable (in terms of frequency of required operator inputs) with other vapor-phase bioreactors. The practicality of the proposed simplification to the operating protocol was confirmed by demonstrating that intermediate metabolites were not accumulating in the TPPB, inorganic nutrient requirements were readily predictable, and that high culture viability could be sustained for prolonged cell retention times (30 days).
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Nielsen DR,Sask KN,McLellan PJ,Daugulis AJdoi
10.1007/s00449-006-0071-2subject
Has Abstractpub_date
2006-10-01 00:00:00pages
229-40issue
4eissn
1615-7591issn
1615-7605journal_volume
29pub_type
杂志文章abstract：:Understanding process parameter interactions and their effects on mammalian cell cultivations is an essential requirement for robust process scale-up. Furthermore, knowledge of the relationship between the process parameters and the product critical quality attributes (CQAs) is necessary to satisfy quality by design g...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1693-7
更新日期：2017-02-01 00:00:00
abstract：:Cellulosic sludge from paper mills making bleached products can be enzymatically converted to glucose. A kinetic model that accounts for product inhibition was used to estimate the cost:benefits of the process. In the proposed scheme, the sludge is enzymatically hydrolyzed in a sequence of CSTRs, the ash separated, an...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0856-4
更新日期：2013-08-01 00:00:00
abstract：:The objective of this study was to evaluate ethanol production and bioadsorption with four red seaweeds, Gelidium amansii, Gracilaria verrucosa, Kappaphycus alvarezii and Eucheuma denticulatum. To produce ethanol, thermal acid hydrolysis, enzymatic saccharification and fermentation was carried out. After pretreatment,...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1571-3
更新日期：2016-06-01 00:00:00
abstract：:Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased th...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-009-0318-9
更新日期：2009-08-01 00:00:00
abstract：:The paper describes a recombinant Schneider 2 (rS2) cell culture and protein expression in a bioreactor. S2 cells were transfected with a plasmid containing a fusion protein (human mu opioid receptor, hMOR, and green fluorescent protein, EGFP) under the control of inducible metallothionein promoter. A bioprocess in a ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0033-0
更新日期：2006-04-01 00:00:00
abstract：:In this study, we attempted to increase butanol/acetone ratio and total solvent productivity in ABE fermentations with corn- and cassava-based media, by consecutively feeding a small amount of butyrate/acetate during solventogenic phase to weaken the metabolic strengths in butyrate/acetate closed-loops. Consecutively ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1133-5
更新日期：2014-08-01 00:00:00
abstract：:In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0951-1
更新日期：2013-11-01 00:00:00
abstract：:Unfolded protein response (UPR) usually happens when expressing heterologous proteins in high level, which may help cells to facilitate protein processing. Here, we evaluated the effects of the UPR activator HAC1p on a raw-starch hydrolyzing α-amylase (Gs4j-amyA), so as to improve heterologous production of the enzyme...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1701-y
更新日期：2017-03-01 00:00:00
abstract：:The performance and operational stability of a pilot-scale static granular bed reactor (SGBR) for the treatment of dairy processing wastewater were investigated under a wide range of organic and hydraulic loading rates and temperature conditions. The SGBR achieved average chemical oxygen demand (COD), biological oxyge...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1275-5
更新日期：2015-02-01 00:00:00
abstract：:A novel and highly stable biomimetic oxidase sensor system was designed for catehol detection. FePP used as biomimetic horseradish peroxidase (HRP) was immobilized onto modified multi-walled carbon nanotubes (MWCNTs). Functional groups such as -OH, -NH2 and -COOH were introduced onto the surface of MWCNTs to provide b...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-018-2032-y
更新日期：2019-02-01 00:00:00
abstract：:Lysozyme is a lytic enzyme, which has antimicrobial activity. It has been used for food and pharmaceutical applications. This study was undertaken to evaluate fed-batch and continuous fermentations for the human lysozyme production in biofilm reactor. Results showed that addition of lactose the mid-log phase to make t...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1483-7
更新日期：2015-12-01 00:00:00
abstract：:In this paper, we build bounded error observers for a common class of partially known bioreactor models. The main idea is to construct hybrid bounded observers "between" high gain observer, which has an adjustable convergence rate but requires perfect knowledge of the model, and asymptotic observer which is very robus...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-005-0409-1
更新日期：2005-08-01 00:00:00
abstract：:Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-020-02463-w
更新日期：2020-11-04 00:00:00
abstract：:A solvent-free biocatalytic process for the synthesis of high quality cetyl laurate, myristate, palmitate and stearate has been optimized. This enzymatic procedure follows the fundamental principles of the Green Chemistry and lead to sustainable products, which can be labeled as natural and conform to the principal re...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1545-5
更新日期：2016-04-01 00:00:00
abstract：:Microbial lipid produced using yeast fermentation with inexpensive carbon sources such as lignocellulosic hydrolyzate can be an alternative feedstock for biodiesel production. Several inhibitors that can be generated during acid hydrolysis of lignocellulose were added solely or together into the culture medium to stud...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0684-6
更新日期：2012-08-01 00:00:00
abstract：:Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO₂/H₂ blend. Acetate production and spore formation were eliminated from Clostridium sp....
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-0991-6
更新日期：2014-02-01 00:00:00
abstract：:D-Amino acid oxidase is an important biocatalyst used in a variety of fields, and its economically justified level recombinant expression in Escherichia coli has not been established. To accomplish this, after a single Phe54Tyr substitution, fusion proteins of D-amino acid oxidase from Trigonopsis variabilis (TvDAO) w...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-013-1123-z
更新日期：2014-08-01 00:00:00
abstract：:The biological production of butanol has become an important research field and thanks to genome sequencing and annotation; genome-scale metabolic reconstructions have been developed for several Clostridium species. This work makes use of the iCAC490 model of Clostridium acetobutylicum ATCC 824 to analyze its metaboli...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-015-1513-5
更新日期：2016-02-01 00:00:00
abstract：:Population heterogeneity is omnipresent in all bioprocesses even in homogenous environments. Its origin, however, is only so well understood that potential strategies like bet-hedging, noise in gene expression and division of labour that lead to population heterogeneity can be derived from experimental studies simulat...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章,评审
doi：10.1007/s00449-018-1922-3
更新日期：2018-07-01 00:00:00
abstract：:The production of chitinases and hydrophobins from Lecanicillium lecanii was influenced by the cultivation method and type of carbon source. Crude enzyme obtained from solid-substrate culture presented activities of exochitinases (32 and 51 kDa), endochitinases (26 kDa), β-N-acetylhexosaminidases (61, 80, 96 and 111 k...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0517-z
更新日期：2011-08-01 00:00:00
abstract：:The growth rate and desulfurization capacity accumulated by the cells during the growth of Pseudomonas putida KTH2 under different oxygen transfer conditions in a stirred and sparged tank bioreactor have been studied. Hydrodynamic conditions were changed using different agitation conditions. During the culture, severa...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-016-1536-6
更新日期：2016-04-01 00:00:00
abstract：:A whole-cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the pro...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-011-0653-5
更新日期：2012-06-01 00:00:00
abstract：:A metabolic network was constructed for the Acremonium chrysogenum cultivation fed with soybean oil. Metabolic flux analysis indicated that the shift from exponential growth to rapid cephalosporin C (CPC) formation was accompanied by 1.63- and 5-fold carbon flux enlargement in TCA cycle and glyoxylate by-pass, respect...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0439-1
更新日期：2010-11-01 00:00:00
abstract：:The effects of biological factors including dissolved oxygen (DO), pH, carbon/nitrogen (C/N) and hydraulic retention times (HRT) on the performance of simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) were investigated. A low DO was found to be advantageous to the SN...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02374-w
更新日期：2020-10-01 00:00:00
abstract：:Bio-catalytic in vitro multistep reactions can be combined in a single step in one pot by optimizing multistep reactions under identical reaction condition. Using this analogy, the process of making PEGylated insulin, IN-105, was simplified. Instead of taking the purified active insulin bulk powder as the starting mat...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0722-4
更新日期：2012-10-01 00:00:00
abstract：:This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0710-8
更新日期：2012-09-01 00:00:00
abstract：:A novel suspended carrier with porous surface was firstly prepared by coating a sponge on the inside and outside of a hard polyethylene ring. Herein the effects of the sponge thickness (0, 2, 4, 6 mm) and pore size (17, 45, 85 pores per inch, ppi) on the performance of the start-up stage in moving bed biofilm reactor ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-014-1266-6
更新日期：2015-02-01 00:00:00
abstract：:Vitamin B12 is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B12 biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-020-02355-z
更新日期：2020-10-01 00:00:00
abstract：:Experiments carried out with the dissolved oxygen tension (DOT) maintained during fermentation at 0, 10, 50, 70 and 100% showed a direct effect of the dissolved oxygen levels on weissellin A production with no correlative increase on biomass. An estimate of the yield of weissellin A per gram biomass revealed the 50% D...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-012-0689-1
更新日期：2012-08-01 00:00:00
abstract：:The aim of the present study was to investigate the feasibility of several food-processing wastes as support substrate for lipolytic enzymes production by the fungus Rhizopus oryzae under solid-state conditions. Different experiments were conducted to select the variables that allow obtaining high levels of lipolytic ...
journal_title：Bioprocess and biosystems engineering
pub_type： 杂志文章
doi：10.1007/s00449-010-0416-8
更新日期：2010-10-01 00:00:00
