Abstract:
:The paper describes a recombinant Schneider 2 (rS2) cell culture and protein expression in a bioreactor. S2 cells were transfected with a plasmid containing a fusion protein (human mu opioid receptor, hMOR, and green fluorescent protein, EGFP) under the control of inducible metallothionein promoter. A bioprocess in a bioreactor with 5% dissolved oxygen, 27 degrees C and 120 rpm enabled the cell culture to attain 5.3x10(7 )viable cells/mL at 96 h. The induction decreased the cell multiplication (2.5x10(7) viable cells/mL at 72 h). Glutamine and glucose and low levels of lactate were consumed. A fast recombinant protein synthesis took place and, at 6 h of induction, 2x10(4) receptors/cell could be detected by a functional binding assay. Fluorescence measurements showed a progressive increase of recombinant protein expression with a maximal value of 1.26x10(5) fluo counts/s at 24 h of induction. The data shown in this paper indicate a practical and scaleable cell culture bioprocess procedure for the preparation of recombinant proteins expressed in S2 cells.
journal_name
Bioprocess Biosyst Engjournal_title
Bioprocess and biosystems engineeringauthors
Brillet K,da Conceição MM,Pattus F,Pereira CAdoi
10.1007/s00449-005-0033-0subject
Has Abstractpub_date
2006-04-01 00:00:00pages
291-3issue
5eissn
1615-7591issn
1615-7605journal_volume
28pub_type
杂志文章abstract::A strain designated M866, producing kojic acid with a high yield, was obtained by combining induced mutation using ion beam implantation and ethyl methane sulfonate treatment of a wild type strain of Aspergillus oryzae B008. The amount of kojic acid produced by the strain M866 in a shaking flask was 40.2 g/L from 100 ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-013-1081-5
更新日期:2014-06-01 00:00:00
abstract::A serine alkaline protease from a newly isolated alkaliphilic Bacillus altitudinis GVC11 was purified and characterized. The enzyme was purified to homogeneity by acetone precipitation, DEAE-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. The molecular weight o...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-010-0483-x
更新日期:2011-05-01 00:00:00
abstract::Penicillin G acylase (PGA) from Kluyvera citrophila immobilized on Amberzyml was used for enantioselective hydrolysis of N-phenylacetylated-DL-tert-leucine (N-Phac-DL-Tle) to produce L-tert-leucine (L-Tle). The effects of various organic cosolvents on hydrolysis of N-Phac-DL-Tle have been investigated in aqueous-cosol...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-005-0027-y
更新日期:2006-04-01 00:00:00
abstract::One of the problems to be solved in attaining the full potentials of hematopoietic stem cell (HSC) applications is the limited availability of the cells. Growing HSCs in a bioreactor offers an alternative solution to this problem. Besides, it also offers the advantages of eliminating labour intensive process as well a...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-010-0449-z
更新日期:2011-01-01 00:00:00
abstract::Escherichia coli AFP111, a pflB, ldhA, ptsG triple mutant of E. coli W1485, can be recovered for additional succinate production in fresh medium after two-stage fermentation (an aerobic growth stage followed by an anaerobic production stage). However, the specific productivity is lower than that of two-stage fermentat...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-010-0484-9
更新日期:2011-05-01 00:00:00
abstract::An extractive biocatalytic method of aqueous two-phase system was employed for stereoinversing (R)-1-phenyl-1,2-ethanediol into (S)-1-phenyl-1,2-ethanediol by Candida parapsilosis CCTCC M203011. It was observed that substrate and product inhibitions in microbial stereoinversion through one-pot oxidation and reduction ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-009-0334-9
更新日期:2010-03-01 00:00:00
abstract::Chemical absorption-biological reduction (BioDeNOx), which uses Fe(II)(EDTA) as a complexing agent for promoting the mass transfer efficiency of NO from gas to water, is a promising technology for removing nitric oxide (NO) from flue gases. The carbon source and pH are important parameters for Fe(II)(EDTA)-NO (the pro...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1378-7
更新日期:2015-07-01 00:00:00
abstract::4''-O-isovalerylspiramycins are the major components of bitespiramycin complex consisting of a group of 4''-O-acylated spiramycins. The availability of isovaleryl group, usually in vivo derived from leucine, one of the branched-chain amino acids, affects the content of isovaleryispiramycin significantly. In this study...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-009-0320-2
更新日期:2010-02-01 00:00:00
abstract::Population heterogeneity is omnipresent in all bioprocesses even in homogenous environments. Its origin, however, is only so well understood that potential strategies like bet-hedging, noise in gene expression and division of labour that lead to population heterogeneity can be derived from experimental studies simulat...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章,评审
doi:10.1007/s00449-018-1922-3
更新日期:2018-07-01 00:00:00
abstract::Organic solid waste composting is a complex process that involves many coupled physical, chemical and biological mechanisms. To understand this complexity and to ease in planning, design and management of the composting plant, mathematical model for simulation is usually applied. The aim of this paper is to develop a ...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1492-6
更新日期:2016-01-01 00:00:00
abstract::In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and tempera...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1465-9
更新日期:2015-12-01 00:00:00
abstract::Menaquinone-7 (MK-7), a subtype of vitamin K, has received a significant attention due to its effect on improving bone and cardiovascular health. Current fermentation strategies, which involve static fermentation without aeration or agitation, are associated with low productivity and scale-up issues and hardly justify...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1807-x
更新日期:2017-10-01 00:00:00
abstract::This work studies the sequential execution of dilute sulfuric acid (DAP) (0.1-0.75 %, v/v) and dilute sodium hydroxide (AKP) (0.25-3 %, w/v) [i.e., DAP followed by AKP (DAP+AKP) and vice versa (AKP+DAP)] at low temperatures (<121 °C) and short reaction times (5-60 min) for maximizing sugar recovery in the enzymatic hy...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1355-1
更新日期:2015-06-01 00:00:00
abstract::An alternating pumped sequencing batch biofilm reactor (APSBBR) system was developed to treat small-scale domestic wastewater. This laboratory system had two reactor tanks, Reactor 1 and Reactor 2, with two identical plastic biofilm modules in each reactor. Reactor 1 of the APSBBR had five operational phases--fill, an...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-005-0038-8
更新日期:2006-04-01 00:00:00
abstract::The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH(4) reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thromb...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-010-0434-6
更新日期:2010-11-01 00:00:00
abstract::The effect of several solvents present in a biphasic reaction system on cells of Rhodococcus erythropolis DCL14, Xanthobacter Py2, Arthrobacter simplex and Mycobacterium sp. NRRL B-3805 was evaluated. These four strains have been widely exploited, from bioremediation to the production of fine chemicals, in two-phase r...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-004-0381-1
更新日期:2004-12-01 00:00:00
abstract::Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant, which finds its way to the receiving water body due to the incapability of conventional wastewater treatment systems to completely remove it. A hybrid treatment system consisting of upflow microbial fuel cell (MFC) with titanium dioxide (TiO2) as a phot...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-020-02396-4
更新日期:2020-11-01 00:00:00
abstract::Experiments carried out with the dissolved oxygen tension (DOT) maintained during fermentation at 0, 10, 50, 70 and 100% showed a direct effect of the dissolved oxygen levels on weissellin A production with no correlative increase on biomass. An estimate of the yield of weissellin A per gram biomass revealed the 50% D...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-012-0689-1
更新日期:2012-08-01 00:00:00
abstract::The biological production of butanol has become an important research field and thanks to genome sequencing and annotation; genome-scale metabolic reconstructions have been developed for several Clostridium species. This work makes use of the iCAC490 model of Clostridium acetobutylicum ATCC 824 to analyze its metaboli...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1513-5
更新日期:2016-02-01 00:00:00
abstract::Understanding process parameter interactions and their effects on mammalian cell cultivations is an essential requirement for robust process scale-up. Furthermore, knowledge of the relationship between the process parameters and the product critical quality attributes (CQAs) is necessary to satisfy quality by design g...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-016-1693-7
更新日期:2017-02-01 00:00:00
abstract::Organic matters are directly converted to electricity by microorganisms in microbial fuel cells (MFC). Modeling the performance of MFC sheds light on the behavior of MFC in various operational conditions (e.g. pH and temperature). In the present research, three voltage losses were considered for modeling of the MFC po...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-019-02235-1
更新日期:2020-03-01 00:00:00
abstract::Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich sourc...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章,评审
doi:10.1007/s00449-020-02463-w
更新日期:2020-11-04 00:00:00
abstract::In this study, aiming to improve the economic feasibility of acetone-butanol-ethanol (ABE) fermentation process, generate valuable products and extend the product chain, esterification catalyzed by Candida sp. 99-125 cells was hybrid with the ABE fermentation-gas-stripping integration system. The gas-stripping condens...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1960-x
更新日期:2018-09-01 00:00:00
abstract::Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the sepa...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1851-6
更新日期:2018-01-01 00:00:00
abstract::Microalgal carotenoids are attractive health ingredients, but their production should be optimized to improve cost-effectiveness. Understanding cellular physiology centered on carotenoid synthesis is the prerequisite for this work. Therefore, systematic correlation analyses were conducted among chlorophyll, carotenoid...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-018-1957-5
更新日期:2018-09-01 00:00:00
abstract::Rice straw is one of the most abundant renewable energy sources available. Through anaerobic acidogenesis, the substance of rice straw can be converted to volatile fatty acids (VFAs). VFAs itself is of value and is a precursor to biofuels. Hence, it can be converted to mixed alcohols by addition of hydrogen, and biodi...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-015-1357-z
更新日期:2015-06-01 00:00:00
abstract::In this study, hydrolysis of marine algal biomass Kappaphhycus alvarezii using two different acid catalysts was examined with the goal of identifying optimal reaction conditions for the formation of sugars and by-products. K. alvarezii were hydrolyzed by autoclave using sulfuric acid or hydrochloric acid as catalyst w...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-011-0609-9
更新日期:2012-01-01 00:00:00
abstract::Development of highly efficient anode is critical for enhancing the power output of microbial fuel cells (MFCs). The aim of this work is to investigate whether modification of carbon paper (CP) anode with graphene (GR) via layer-by-layer assembly technique is an effective approach to promote the electricity generation...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-014-1148-y
更新日期:2014-09-01 00:00:00
abstract::Staphylococcal enterotoxin B (SEB) is one of many toxins produced by the Gram-positive bacterium Staphylococcal aureus. While SEB is known as the causative agent of certain food poisonings it is also considered abiological select agent. Thus, rapid and accurate identification of SEB during either surveillance or in re...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-007-0168-2
更新日期:2008-06-01 00:00:00
abstract::During the production of bio-based bulk chemicals, such as lactic acid (LA), organic impurities have to be removed to produce a ready-to-market product. A capillary electrophoresis method for the simultaneous detection of LA and organic impurities in less than 10 min was developed. LA and organic impurities were detec...
journal_title:Bioprocess and biosystems engineering
pub_type: 杂志文章
doi:10.1007/s00449-017-1761-7
更新日期:2017-07-01 00:00:00